MitoProbe™ TMRM Assay-Kit für die Durchflusszytometrie

Zitierungen und Referenzen (4)

Thermo Scientific™

MitoProbe™ TMRM Assay-Kit für die Durchflusszytometrie

Katalognummer	Menge
M20036	100 Assays

Katalognummer M20036

Preis (EUR)

238,65

Precio exclusivo en nuestra web

257,00

Ersparnis 18,35 (7%)

Menge:

100 Assays

Preis (EUR)

238,65

Precio exclusivo en nuestra web

257,00

Ersparnis 18,35 (7%)

Das MitoProbe TMRM Assay-Kit enthält Tetramethylrhodamin-Methylester (TMRM) für den Nachweis des mitochondrialen Membranpotentialzustands und CCCP für die Induktion der mitochondrialen Membrandepolarisation, falls gewünscht.

• Untersuchung des mitochondrialen Membranpotenzials in lebenden Zellen
• Schnelles, einfaches Protokoll

Die Bestimmung des mitochondrialen Gesundheitszustands kann durch den Nachweis von Änderungen des mitochondrialen Membranpotenzials erreicht werden. In einer gesunden Zelle mit aktiven Mitochondrien wird TMRM leicht sequestriert, wodurch ein rot-oranges Fluoreszenzsignal abgegeben wird. Wenn Apoptose induziert oder eine Behandlung wie CCCP angewendet wird, wird die mitochondriale Membran depolarisiert und das TMRM-Signal nimmt ab.

Specifications

NachweisverfahrenFluoreszent

FormatFläschchen

Menge100 Assays

VersandbedingungNasseis

Emission561

Zur Verwendung mit (Anwendung)TMRM-Assay

Zur Verwendung mit (Geräte)Durchflusszytometer

ProdukttypDurchflusszytometrie-Assaykit

Unit SizeEach

Inhalt und Lagerung

1 Fläschchen TMRM (100 Tests)
1 Fläschchen 50 mM CCCP
Bei -5 °C bis -30 °C lagern.

Häufig gestellte Fragen (FAQ)

In the MitoProbe TMRM Assay Kit for Flow Cytometry, should CCCP be present when adding the TMRM?

Yes. The CCCP should be first added to the cell sample, incubated for 5 mins, and then the TMRM should be added and incubated for another 30 mins.

Find additional tips, troubleshooting help, and resources within our Cell Analysis Support Center.

How does the MitoProbe TMRM Assay Kit for Flow Cytometry (Cat. No.M20036) differ from the Image-iT TMRM Reagent (Cat No. I34361)?

The Image-iT TMRM Reagent is provided as a 100 µM solution of TMRM in DMSO (5 vials, 100 µL in each vial). The MitoProbe TMRM Assay Kit for Flow Cytometry provides the TMRM as a 20 µM stock solution in DMSO (1 vial, 100 µL) and a vial of CCCP. Both kits have the stock solutions as a 1000X concentration, but final working concentrations required for imaging applications are 5-fold higher than what is needed for flow cytometry analysis.

Find additional tips, troubleshooting help, and resources within our Cell Analysis Support Center.

How is TMRM different from the MitoTracker dyes?

TMRM is a dynamic mitochondrial stain. There is no covalent attachment of TMRM to the mitochondria and because of this, it can move in and out of the mitochondria dependent upon changes in the membrane potential. The MitoTracker dyes on the other hand possess a reactive chloromethyl group that allows for their covalent attachment to mitochondria. As a result, once they accumulate within mitochondria that have a normal membrane potential, they are retained.

Find additional tips, troubleshooting help, and resources within our Cell Analysis Support Center.

Zitierungen und Referenzen (4)

Zitierungen und Referenzen

Abstract

Mitochondrial Delivery of an Anticancer Drug Via Systemic Administration Using a Mitochondrial Delivery System That Inhibits the Growth of Drug-Resistant Cancer Engrafted on Mice.

Authors:Yamada Y, Munechika R, Satrialdi Kubota F, Sato Y, Sakurai Y, Harashima H

Journal:J Pharm Sci

PubMed ID:32376272

'Mitochondrial delivery of an anticancer drug targeting cancer cells would eventually result in cell death. To achieve this, a drug delivery system targeting mitochondria is needed. We recently developed a MITO-Porter, a liposome that delivers its cargo to mitochondria. We reported that such a MITO-Porter could deliver doxorubicin (DOX), an ... More

Zidovudine-Mediated Autophagy Inhibition Enhances Mitochondrial Toxicity in Muscle Cells.

Authors:Lin H, Stankov MV, Hegermann J, Budida R, Panayotova-Dimitrova D, Schmidt RE, Behrens GMN,

Journal:Antimicrob Agents Chemother

PubMed ID:30373793

Nucleoside reverse transcriptase inhibitors (NRTI), such as zidovudine (AZT), are constituents of HIV-1 therapy and are used for the prevention of mother-to-child transmission. Prolonged thymidine analogue exposure has been associated with mitochondrial toxicities to heart, liver, and skeletal muscle. We hypothesized that the thymidine analogue AZT might interfere with autophagy ... More

Lectin-induced oxidative stress in human platelets.

Authors:Signorello MG, Ravera S, Leoncini G

Journal:Redox Biol

PubMed ID:32063518

Previously we have shown that wheat germ agglutinin (WGA) and, with minor potency, Phaseolus vulgaris agglutinin (PHA), but not lens culinarian agglutinin (LCA), induce platelet aggregation, through the PLC?2 activation by the concerted action of src/syk and PI3K/BTK pathways. In this study, we have investigated platelet oxidative stress induced by ... More

Rapid Death of Follicular B Cells and Burkitt Lymphoma Cells Effectuated by Xbp1s.

Authors:Chen YT, Kung JT

Journal:J Immunol

PubMed ID:32376649

BCR-mediated tonic signaling is an indispensable requirement for the survival of follicular B (FOB) cells and Burkitt lymphoma (BL) cells. FOB cells of the I-A