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Zitierungen und Referenzen (23)
Invitrogen™
PDAM (1-Pyrenyldiazomethane)
Das fluoreszierende Diazomethyl-Derivat PDAM (1-Pyrenyldiazomethan) wird häufig zur Derivatisierung von Biomolekülen verwendet. Diazoalkan reagiert mit der Zugabe von Katalyse undWeitere Informationen
| Katalognummer | Menge |
|---|---|
| P1405 | 25 mg |
Katalognummer P1405
Preis (EUR)
447,00
Each
Menge:
25 mg
Preis (EUR)
447,00
Each
Das fluoreszierende Diazomethyl-Derivat PDAM (1-Pyrenyldiazomethan) wird häufig zur Derivatisierung von Biomolekülen verwendet. Diazoalkan reagiert mit der Zugabe von Katalyse und kann für die direkte Carbonsäure-Modifikation von Proteinen und synthetischen Polymeren verwendet werden. Fluoreszierende Diazoalkane reagieren ebenfalls mit Phosphaten. PDAM wird als Alternative zu ADAM empfohlen, da es eine wesentlich bessere chemische Stabilität aufweist. Darüber hinaus beträgt die Nachweisgrenze für Konjugate mit PDAM Berichten zufolge etwa 20 bis 30 Femtomole, was fünfmal besser ist als für ADAM-Konjugate.
Nur für Forschungszwecke. Nicht zur Verwendung bei diagnostischen Verfahren.
Specifications
Chemische ReaktivitätCarbonsäure
Marker oder FarbstoffPyren
ProdukttypPDAM
Menge25 mg
Reaktiver TeilDiazoalkan
VersandbedingungNasseis
MarkertypKlassische Farbstoffe
Unit SizeEach
Inhalt und Lagerung
Bei -5 bis -30 °C lagern und vor Licht schützen.
Zitierungen und Referenzen (23)
Zitierungen und Referenzen
Abstract
Omega oxidation of 3-hydroxy fatty acids by the human CYP4F gene subfamily enzyme CYP4F11.
Journal:J Lipid Res
PubMed ID:18065749
'Long-chain 3-hydroxydicarboxylic acids (3-OHDCAs) are thought to arise via beta-oxidation of the corresponding dicarboxylic acids (DCAs), although long-chain DCAs are neither readily transported into nor beta-oxidized in mitochondria. We thus examined whether omega-hydroxylation of 3-hydroxy fatty acids (3-OHFAs), formed via incomplete mitochondrial oxidation, is a more likely pathway for 3-OHDCA
Headspace solid-phase microextraction with 1-pyrenyldiazomethane in-fibre derivatisation for analysis of faecal short-chain fatty acids.
Journal:J Chromatogr B Biomed Sci Appl
PubMed ID:10437678
'Short chain fatty acids (SFCAs) are nutritionally important products of colonic bacteria. Their analysis in faeces is problematic. We report a headspace solid-phase microextraction procedure in which faecal SCFAs are derivatised on the fibre in-situ with 1-pyrenyldiazomethane. With this method sharp, well-resolved chromatographic peaks were obtained with no interferences. Inclusion
[Highly effective separation and highly sensitive detection for clinical chemistry and biochemistry]
Journal:Yakugaku Zasshi
PubMed ID:9513578
'Highly effective separation and highly sensitive detection reagents for clinical chemistry and biochemistry were developed and their applications were investigated. The sensitive detection of carboxylic acids was accomplished using 9-anthryldiazomethane (ADAM) which gave intensely fluorescent derivatives from carboxylic acids without catalysts or heating. 1-Pyrenyldiazomethane was then synthesized and proved to
Application of capillary electrophoresis with laser-induced fluorescence detection for routine determination of methylmalonic acid in human serum.
Journal:Anal Chem
PubMed ID:7762817
'Methylmalonic acid (MMA) in serum is an established marker of cobalamin deficiency. MMA and other short-chain dicarboxylic acids react with 1-pyrenyldiazomethane to form stable, highly fluorescent 1-pyrenylmethyl monoesters. We have analyzed these esters in human blood by capillary electrophoresis (CE) combined with laser-induced fluorescence detection, and here we describe our
Enhancement of hydrolytic activity of sphingolipid ceramide N-deacylase in the aqueous-organic biphasic system.
Journal:J Lipid Res
PubMed ID:10787446
'Lysoglycosphingolipids were produced from glycosphingolipids by using sphingolipid ceramide N-deacylase, which cleaves the N-acyl linkage between fatty acids and sphingosine bases in various glycosphingolipids. The enzyme reaction was done in a biphasic media prepared with water;-immiscible organic solvent and aqueous buffer solution containing the enzyme. We investigated the effects of