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Fluorescein-Ubiquitin
Zitierungen und Referenzen (1)

Fluorescein-Ubiquitin

Fluorescein-labeled ubiquitin for use as a general research tool or in high throughput assays to monitor protein ubiquitination. Due toWeitere Informationen
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KatalognummerMenge
PV437750 μg
PV4378500 μg
Katalognummer PV4377
Preis (EUR)
370,00
Each
Menge:
50 μg
Preis (EUR)
370,00
Each
Fluorescein-labeled ubiquitin for use as a general research tool or in high throughput assays to monitor protein ubiquitination. Due to the method in labeling the ubiquitin protein, all of the lysines with the protein are available for further conjugation to form poly-ubiquitin chains.

For use in combination with FRET donors including LanthaScreen™ Tb-Ubiquitin (PV4375) or a LanthaScreen™ antibody including Tb-anti-GST (PV4216) or Tb-anti-ubiquitin-FK2 (PV4751).
For Research Use Only. Not for use in diagnostic procedures.
Specifications
NachweisverfahrenFluorescence
Anregung/Emission340⁄490, 520
Zur Verwendung mit (Geräte)Microplate Reader
MarkertypClassic Dyes
Marker oder FarbstoffFITC (Fluorescein)
Menge50 μg
AnzeigewertEnd Point
VersandbedingungDry Ice
SubstrateigenschaftenProtein-Based Substrate
VerfahrenTR-FRET
KonjugatFluorescein
FormLiquid
Unit SizeEach
Inhalt und Lagerung
Fluorescein-labeled ubiquitin in HEPES buffered saline. Store at -20°C.

Zitierungen und Referenzen (1)

Zitierungen und Referenzen
Abstract
TR-FRET-based high-throughput screening assay for identification of UBC13 inhibitors.
Authors:Madiraju C, Welsh K, Cuddy MP, Godoi PH, Pass I, Ngo T, Vasile S, Sergienko EA, Diaz P, Matsuzawa S, Reed JC,
Journal:J Biomol Screen
PubMed ID:22034497
UBC13 is a noncanonical ubiquitin conjugating enzyme (E2) that has been implicated in a variety of cellular signaling processes due to its ability to catalyze formation of lysine 63-linked polyubiquitin chains on various substrates. In particular, UBC13 is required for signaling by a variety of receptors important in immune regulation, ... More