Zitierungen und Referenzen (305)

Invitrogen™

Resazurin, Sodium Salt

Resazurin, Natriumsalz

Katalognummer	Menge
R12204	10 mg

Katalognummer R12204

Preis (EUR)

297,00

Menge:

10 mg

Preis (EUR)

297,00

Das nicht fluoreszierende Resazurin kann als fluorogener Oxidationsreduktionsindikator in einer Vielzahl von Zellen, einschließlich Bakterien, Hefen und Eukaryoten, durch Durchflusszytometrie, Fluoreszenzmikroskopie und Hochdurchsatz-Screening eingesetzt werden. Das rot-fluoreszierende Produkt Resorufin weist Absorptions-/Emissionsmaxima von ∼575/585 nm auf.

Nur für Forschungszwecke. Nicht zur Verwendung bei diagnostischen Verfahren.

Specifications

BeschreibungResazurin, Natriumsalz

NachweisverfahrenFluoreszent

FarbstofftypSalz

FormFest

FormatTube

Menge10 mg

VersandbedingungRaumtemperatur

FarbeRot

Emission585 nm

Excitation Wavelength Range575 nm

Zur Verwendung mit (Anwendung)Viabilitätsassay

Zur Verwendung mit (Geräte)Mikrotiterplatten-Lesegerät

ProduktlinieInvitrogen

ProdukttypResazurin

Unit SizeEach

Inhalt und Lagerung

Lagerung bei Raumtemperatur und vor Licht geschützt

Häufig gestellte Fragen (FAQ)

How do alamarBlue reagent and PrestoBlue reagent differ from resazurin and C12-resazurin?

alamarBlue reagent and PrestoBlue reagent contain resazurin in a proprietary stabilizing formulation that allows for a convenient “mix, incubate, and read” protocol. PrestoBlue reagent is an improvement in the formulation of alamarBlue reagent that allows for much faster staining (typically 10 minutes vs. 1-4 hours to obtain a similar signal and sensitivity). C12-resazurin is a derivative of resazurin that has better cellular retention and thus allows for analysis on a flow cytometer and multiplexing with viability indicators and other biomarkers.

Find additional tips, troubleshooting help, and resources within our Cell Analysis Support Center.

Zitierungen und Referenzen (305)

Zitierungen und Referenzen

Abstract

Rapid screening of natural products for antimycobacterial activity by using luciferase-expressing strains of Mycobacterium bovis BCG and Mycobacterium intracellulare.

Authors:Shawar RM,Humble DJ,Van Dalfsen JM,Stover CK,Hickey MJ,Steele S,Mitscher LA,Baker W

Journal:Antimicrobial agents and chemotherapy

PubMed ID:9055994

The object of this study was to investigate the ability of a rapid luciferase assay to detect antimycobacterial activity in plant extracts. Recombinant strains of Mycobacterium bovis BCG (rBCG) and Mycobacterium intracellulare expressing firefly luciferase were used as the test organisms. Assays were conducted in a 96-well minitube format under ... More

Inhibition of growth and sensitization to cisplatin-mediated killing of ovarian cancer cells by polyphenolic chemopreventive agents.

Authors:Chan MM, Fong D, Soprano KJ, Holmes WF, Heverling H

Journal:J Cell Physiol

PubMed ID:12447990

'The polyphenolic compounds curcumin and quercetin increased sensitivity of ovarian cancer cells (CAOV3 and SKOV3) to cisplatin. The effect was obtained when the compounds were added simultaneously with cisplatin, as well as when they were added 24 h before. High serum levels of certain cytokines, for example interleukin-6 (IL-6), have ... More

Mechanism of target cell recognition by natural killer cells: characterization of a novel triggering molecule restricted to CD3- large granular lymphocytes.

Authors:Frey JL, Bino T, Kantor RR, Segal DM, Giardina SL, Roder J, Anderson S, Ortaldo JR

Journal:J Exp Med

PubMed ID:1720812

'In an attempt to identify a molecule in target recognition by CD3- large granular lymphocytes (LGL), we have generated a rabbit antiidiotypic (anti-ID) serum against a monoclonal antibody (mAb 36) that reacted with the cell membrane of K562. Flow cytometry analysis demonstrated that the anti-ID serum bound selectively to CD3- ... More

Functional in vivo MHC class II loading by endogenously synthesized glycoprotein during viral infection.

Authors:Oxenius A, Bachmann MF, Mathis D, Benoist C, Zinkernagel RM, Hengartner H

Journal:J Immunol

PubMed ID:9190921

'MHC class II presentation of antigenic peptides derived from soluble proteins is usually preceded by antigenic uptake via (nonreceptor-mediated) endocytosis by professional APCs, followed by processing in endosomal compartments. Although in vitro alternative pathways for MHC class II loading have been described for certain intracellularly synthesized proteins, the importance of ... More

Cell proliferation enhances entry of Listeria monocytogenes into intestinal epithelial cells by two proliferation-dependent entry pathways.

Authors:Velge P, Bottreau E, Van-Langendonck N, Kaeffer B

Journal:J Med Microbiol

PubMed ID:9511817

'Bacterial entry into intestinal host cells is the result of a fairly sophisticated manipulation of host cell machinery by the pathogens. To study further the potential cell target of Listeria spp., the in-vitro entry of L. monocytogenes strains into intestinal cells was examined in relation to the metabolism, proliferation and ... More

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