Der thiolreaktive Rhodamin Green™-X Succinimidylester kann verwendet werden, um grün-fluoreszierende Biokonjugate mit Anregungs-/Emissionsmaxima bei ∼502/527 nm zu erzeugen. Dieser FarbstoffWeitere Informationen
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Katalognummer
Menge
R6113
auch als R-6113 bezeichnet
5 mg
Katalognummer R6113
auch als R-6113 bezeichnet
Preis (EUR)
700,00
Each
Menge:
5 mg
Preis (EUR)
700,00
Each
Der thiolreaktive Rhodamin Green™-X Succinimidylester kann verwendet werden, um grün-fluoreszierende Biokonjugate mit Anregungs-/Emissionsmaxima bei ∼502/527 nm zu erzeugen. Dieser Farbstoff ist das nicht-sulfonierte Analogon des Alexa Fluor™ 488 Farbstoffs.
Nur für Forschungszwecke. Nicht zur Verwendung bei diagnostischen Verfahren.
Specifications
Chemische ReaktivitätAmin
Emission527
Anregung502
Marker oder FarbstoffRhodamine Green™
ProdukttypSuccinimidyll-Ester
Menge5 mg
Reaktiver TeilAktiv-Ester, Succinimidylester
VersandbedingungRaumtemperatur
MarkertypKlassische Farbstoffe
ProduktlinieRhodamine Green
Unit SizeEach
Inhalt und Lagerung
Bei -5 bis -30 °C lagern und vor Licht schützen.
Häufig gestellte Fragen (FAQ)
What is the excitation and emission wavelength for rhodamine?
Rhodamine is a generic term for a wide variety of cationic dyes whose fluorescence emission can range from green, orange to red. The table below lists the excitation and emission maxima (nm), as well as molar extinction coefficients (EC; cm-1 M-1), for various rhodamine dyes (data derived with dye dissolved in methanol).
Flow cytometric quantitation of human opsonin-dependent phagocytosis and oxidative burst responses to meningococcal antigens.
Authors:Lehmann AK, Halstensen A, Bassøe CF
Journal:Cytometry
PubMed ID:9845434
'A one-step flow cytometric (FCM) assay has been developed to quantify both opsonin- and antigen-dependent phagocytosis and intraphagocyte oxidative burst responses. Meningococcal outer membrane structures (OMV) were adsorbed to fluorescent polystyrene beads, opsonized with serum, and exposed to leukocytes. FCM parameters of phagocytosis were evaluated in combinations with oxidative burst ... More
Ultrasensitive detection and identification of fluorescent molecules by FCS: impact for immunobiology.
Authors:Földes-Papp Z, Demel U, Tilz GP
Journal:Proc Natl Acad Sci U S A
PubMed ID:11572995
'An experimental application of fluorescence correlation spectroscopy is presented for the detection and identification of fluorophores and auto-Abs in solution. The recording time is between 2 and 60 sec. Because the actual number of molecules in the unit volume (confocal detection volume of about 1 fl) is integer or zero, ... More
Nonperturbing fluorescent labeling of polysaccharides.
Authors:Meunier F, Wilkinson KJ
Journal:Biomacromolecules
PubMed ID:12099834
'A fluorescent labeling procedure, which does not perturb macromolecular conformations, was employed to bind a rhodamine derivative to the reducing end of several water-soluble polysaccharides by reductive amination in the presence of sodium cyanoborohydride. Fluorescence correlation spectroscopy, atomic force microscopy, and size exclusion chromatography were used to demonstrate that the ... More
Comparison of sieving matrices for on-the-fly fluorescence lifetime detection of dye-labeled DNA fragments.
Authors:Li L, McGown LB
Journal:Fresenius J Anal Chem
PubMed ID:11293703
'Commercially available, replaceable sieving matrices and their solvent modulated forms were evaluated for use in on-the-fly fluorescence lifetime detection of dye-labeled DNA fragments in capillary electrophoresis. The fragments were labeled with dyes that can be excited by the 488 nm line of an argon ion laser and have lifetimes in ... More
Biological and chemical applications of fluorescence correlation spectroscopy: a review.