Zitierungen und Referenzen (4)

Invitrogen™

NI-NTA Agarose

Name: NI-NTA Agarose
SKU: R90101
Price: 378.00 EUR

Ni-NTA-Agarose ist ein nickelbeladenes Affinitätsharz, das zur Reinigung rekombinanter Proteine verwendet werden kann, die eine Polyhistidin-Sequenz (6xHis) enthalten. An dasWeitere Informationen

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Katalognummer	Menge
R90101	10 ml
R90115	25 ml
R90110	100 ml

3 Optionen

Katalognummer R90101

Preis (EUR)

378,00

Each

Menge:

10 ml

Großbestellung oder individuelle Größe anfordern

Preis (EUR)

378,00

Each

Ni-NTA-Agarose ist ein nickelbeladenes Affinitätsharz, das zur Reinigung rekombinanter Proteine verwendet werden kann, die eine Polyhistidin-Sequenz (6xHis) enthalten. An das Harz gebundene Proteine können entweder mit einem Puffer mit niedrigem pH-Wert oder durch Konkurrenz mit Imidazol oder Histidin eluiert werden. Die einstufige Reinigung kann sowohl unter nativen als auch unter denaturierenden Bedingungen durchgeführt werden. Ni-NTA verwendet den chelatbildenden Liganden Nitrilotriessigsäure (NTA) in Verbindung mit einem quervernetzten, 6%igen Agaroseharz, das für den Einsatz in Batch- und Schwerkraftfluss-Anwendungen geeignet ist

Nur für Forschungszwecke. Darf nicht für diagnostische Verfahren eingesetzt werden.

Specifications

Menge10 ml

Stationäre PhaseNi-NTA

SäulentypAffinitätssäule

FormSuspension

TypAgarose

Unit SizeEach

Inhalt und Lagerung

Das Ni-NTA-Harz ist vorgeladen und kann bis zu 50 mg rekombinantes Protein pro 1 ml Harz binden. Es wird als 50%ige Suspension in 30 % Ethanol bereitgestellt. Das Harz erscheint blau, wenn es mit Ni²⁺ geladen wird. Bei +4 °C lagern. Ni-NTA-Harz ist bei ordnungsgemäßer Lagerung garantiert 6 Monate lang stabil.

Häufig gestellte Fragen (FAQ)

Are there other sequences that can bind to nickel more tightly than 6xHis-tagged proteins and how can they be eluted.

Yes, 7xHis-tagged proteins, proteins naturally high in histidine, and other combinations of His and other amino acids will bind. To elute them, you have to increase the concentration of imidazole. Generally these peptides will not contaminate your fraction since they remain on the column. However after multiple uses of the same column, these peptides may reduce the binding capacity of the column.

Find additional tips, troubleshooting help, and resources within our Protein Assays and Analysis Support Center.

Can ProBond or Ni-NTA beads be used for large-scale preparations?

ProBond and Ni-NTA beads can be used in FPLC columns. However, the beads can only withstand low pressure (~43.5 psi max).

Find additional tips, troubleshooting help, and resources within our Protein Purification and Isolation Support Center.

What should the typical protein recovery be when using the Probond Purification System or Ni-NTA Purification system?

Both systems are qualified by purifying 2 mg of myoglobin protein on a column and performing a Bradford assay. Protein recovery must be 75% or higher.

Find additional tips, troubleshooting help, and resources within our Protein Purification and Isolation Support Center.

Zitierungen und Referenzen (4)

Zitierungen und Referenzen

Abstract

A protein kinase encoded by the t complex responder gene causes non-mendelian inheritance

Authors:BERNHARD G. HERRMANN, BIRGIT KOSCHORZ, KARIN WERTZ, K. JOHN MCLAUGHLIN* & ANDREAS KISPERT

Journal:Nature

PubMed ID:10647005

'Males heterozygous for the t-haplotype form of mouse chromosome 17 preferentially transmit the t-chromosome to their progeny. Several distorter/sterility loci carried on the t-haplotype together impair flagellar function in all spermatozoa whereas the responder, Tcr, rescues t-sperm but not wild-type sperm. Thus, t-sperm have anadvantage over wild-type sperm in fertilizing ... More

DNA Polymerase lambda, a Novel DNA Repair Enzyme in Human Cells.

Authors: Garcia-Diaz Miguel; Bebenek Katarzyna; Sabariegos Rosario; Dominguez Orlando; Rodriguez Josana; Kirchhoff Tomas; Garcia-Palomero Esther; Picher Angel J; Juarez Raquel; Ruiz Jose F; Kunkel Thomas A; Blanco Luis;

Journal:J Biol Chem

PubMed ID:11821417

'DNA polymerase lambda (pol lambda) is a novel family X DNA polymerase that has been suggested to play a role in meiotic recombination and DNA repair. The recent demonstration of an intrinsic 5''-deoxyribose-5-phosphate lyase activity in pol lambda supports a function of this enzyme in base excision repair. However, the ... More

A ribozyme that ligates RNA to protein.

Authors: Baskerville Scott; Bartel David P;

Journal:Proc Natl Acad Sci U S A

PubMed ID:12077317

'We have used a combination of in vitro selection and rational design to generate ribozymes that form a stable phosphoamide bond between the 5'' terminus of an RNA and a specific polypeptide. This reaction differs from that of previously identified ribozymes, although the product is analogous to the enzyme-nucleotidyl intermediates ... More

Transduction of MIN6 beta Cells with TAT-Syntaxin SNARE Motif Inhibits Insulin Exocytosis in Biphasic Insulin Release in a Distinct Mechanism Analyzed by Evanescent Wave Microscopy.

Authors: Ohara-Imaizumi Mica; Nakamichi Yoko; Nishiwaki Chiyono; Nagamatsu Shinya;

Journal:J Biol Chem

PubMed ID:12393909

To investigate the in vivo interaction of syntaxin-mediated soluble N-ethylmaleimide-sensitive fusion protein attachment protein receptor (SNARE) assembly and insulin exocytosis in biphasic release, we examined the dynamics of insulin granule motion such as docking and fusion with the plasma membrane when the syntaxin SNARE motif (H3 domain) was transduced into ... More