Unser Alexa Fluor 430 Streptavidin-Konjugat ist eine helle, grün fluoreszierende Sonde (Absorptions-/Emissionsmaxima ∼434/539 nm). Streptavidin, ein nicht glykosyliertes Biotin-bindendes ProteinWeitere Informationen
Have Questions?
Katalognummer
Menge
S11237
auch als S-11237 bezeichnet
1 mg
Katalognummer S11237
auch als S-11237 bezeichnet
Preis (EUR)
437,00
Each
Menge:
1 mg
Preis (EUR)
437,00
Each
Unser Alexa Fluor 430 Streptavidin-Konjugat ist eine helle, grün fluoreszierende Sonde (Absorptions-/Emissionsmaxima ∼434/539 nm). Streptavidin, ein nicht glykosyliertes Biotin-bindendes Protein mit einem nahezu neutralen isoelektrischen Punkt, weist Berichten zufolge weniger unspezifische Bindungen auf als Avidin.
Im Tiefkühlgerät bei -5 bis -30 °C und vor Licht geschützt lagern.
Häufig gestellte Fragen (FAQ)
I am planning to use a fluorescent streptavidin labeled conjugate. What are the storage conditions and shelf life for the lyophilized powder and reconstituted solution?
In the lyophilized powder form, the fluorescent streptavidin labeled conjugate is stable for six months when stored at -20 degrees C, desiccated, and protected from light. The reconstituted solution is stable for approximately six months when stored at 4 degrees C, protected from light, with the addition of sodium azide to a final concentration of 5 mM or thimerosal to 0.2 mM. For longer storage, we recommend dividing the solution into aliquots and freezing at -20 degrees C, protected from light. Avoid repeated freezing and thawing of the solution.
I am planning to use a fluorescent streptavidin labeled conjugate. How should I prepare the working solution of the conjugate?
The fluorescent streptavidin labeled conjugate solution can be made by dissolving the powder in 0.5-1.0 mL of PBS or other suitable buffer. For details, please refer to page 4 of the "Streptavidin and Fluorescent Conjugates of Streptavidin" manual (https://assets.fishersci.com/TFS-Assets/LSG/manuals/mp00888.pdf).
Zitierungen und Referenzen (1)
Zitierungen und Referenzen
Abstract
Detection and quantification of biotinylated proteins using the Storm 840 Optical Scanner.
Authors:Lewis B, Rathman S, McMahon RJ
Journal:J Nutr Biochem
PubMed ID:12770643
'The use of the avidin-biotin interaction is becoming an increasingly common method for the detection of proteins. The use of fluorescence detection with avidin-biotin systems has the potential to greatly increase both the sensitivity and linearity of this type of analysis. In this report, three fluorescent systems were tested for ... More