SYPRO™ Proteingel-Färbemittel

Zitierungen und Referenzen (112)

Invitrogen™

SYPRO™ Proteingel-Färbemittel

Name: SYPRO™ Proteingel-Färbemittel
SKU: S6651
Price: 524.65 EUR

Ansicht ändern

Katalognummer	Farbe	Menge
S6651	Orange	10 x 50 μl
S6650	Orange	500 μl
S12010	Orangefarben	500 μl
S6653	Rot	500 μl
S6654 auch als S-6654 bezeichnet	Rot	10 x 50 μl
S12001	Rubin	200 mL
S12000	Rubin	1 l
S21900	Rubin	5 l
S12000X3	Rubin	3 x 1 l

9 Optionen

Katalognummer S6651

Preis (EUR)

524,65

Online Exclusive

552,00

Ersparnis 27,35 (5%)

Each

Zum Warenkorb hinzufügen

Farbe:

Orange

Menge:

10 x 50 μl

Großbestellung oder individuelle Größe anfordern

Preis (EUR)

524,65

Online Exclusive

552,00

Ersparnis 27,35 (5%)

Each

Zum Warenkorb hinzufügen

SYPRO Ruby Protein-Gelfärbung ist eine hochempfindliche, gebrauchsfertige fluoreszierende Färbung für den Nachweis von durch Polyacrylamid-Gelelektrophorese (PAGE) getrennten Gesamtproteinen. Ideal für den Einsatz in 1D- und 2D-PAGE. Die Empfindlichkeit von SYPRO Ruby Gelfärbung ist mindestens so hoch wie die der besten Silberfärbeverfahren. Gefärbte Proteine können mit einem standardmäßigen UV- oder Blaulicht-Transilluminator oder einem Bildgebungsgerät mit den entsprechenden Filtern oder Lasern betrachtet werden.

Merkmale:
• Einfaches Färbeverfahren: kein Entfärben oder zeitgesteuerte Schritte erforderlich
• Linearer Quantifizierungsbereich über drei Größenordnungen
• Kompatibilität mit Massenspektrometrie und Mikrosequenzierung

Vergleich aller Fluoreszenz-Färbemittel ›

Nur für Forschungszwecke. Darf nicht für diagnostische Verfahren eingesetzt werden.

Specifications

Konzentration5000X in DMSO

DetektionsstelleIn-Gel-Detektion

NachweisverfahrenFluoreszenz

Anregung/Emission300, 470/570 nm

ProduktlinieSYPRO

ProdukttypProteingel-Färbemittel

Menge10 x 50 μl

VersandbedingungRaumtemperatur

ZielmolekülProtein

FarbeOrange

Marker oder FarbstoffSYPRO Orange

Unit SizeEach

Inhalt und Lagerung

Lieferung als 5.000x-Konzentrat in DMSO. Bei Raumtemperatur vor Licht geschützt lagern.

Häufig gestellte Fragen (FAQ)

If I increase the final concentration of my SYPRO Orange or SYPRO Red staining solution above 1X, can I increase the signal of my stained proteins?

No. Dye concentrations higher than 1X do not give better detection. Instead, background fluorescence increases and, as the dye concentration increases, the dye becomes self-quenching and the signal actually decreases.

Find additional tips, troubleshooting help, and resources within our Protein Assays and Analysis Support Center.

Can I dry my SYPRO Ruby, SYPRO Orange, or SYPRO Red stained gels?

Gels stained with SYPRO dyes can be dried between sheets of cellophane, although there is sometimes a slight decrease in sensitivity. If the gels are dried onto paper, the light will scatter and the sensitivity will decrease. Other plastics are not recommended, as the plastic typically used is not transparent to UV light.

Find additional tips, troubleshooting help, and resources within our Protein Assays and Analysis Support Center.

Can I pre-stain proteins with SYPRO Ruby, SYPRO Orange or SYPRO Red Protein Gel Stains and then run them through a gel?

No. Loading solutions contain so much SDS that SYPRO Ruby, SYPRO Orange and SYPRO Red dyes simply localize in the free SDS and bind very little of the proteins. Proteins can be covalently pre-labeled with ATTO-TAG CBQCA (Cat. No. A2333), DDAO succinimidyl ester (Cat. No. C34553) or TAMRA-succinimidyl ester (Cat. No. C2211) dyes, or the TC-FLAsH Expression Analysis Detection Kits (Cat. No. A10067 for orange fluorescence, Cat. No. A10068 for red fluorescence) prior to electrophoresis without affecting protein migration through the gel.

SYPRO Orange or SYPRO Red Protein Gel Stain can be diluted 5000-fold into the cathode (upper) buffer tank to stain proteins during electrophoresis without affecting migration. The problem with doing this is that there is considerable background fluorescence in the gels from the dye interacting with SDS. This can be reduced after electrophoresis by destaining in 7.5% acetic acid for 15-60 minutes. This method also results in poorer protein sensitivity than the standard post-staining method, requires the same amount of time before the gel can be imaged, and contaminates the electrophoresis apparatus.

Find additional tips, troubleshooting help, and resources within our Protein Assays and Analysis Support Center.

Zitierungen und Referenzen (112)

Zitierungen und Referenzen

Abstract

LXXLL-related motifs in Dax-1 have target specificity for the orphan nuclear receptors Ad4BP/SF-1 and LRH-1.

Authors:Suzuki T, Kasahara M, Yoshioka H, Morohashi K, Umesono K

Journal:Mol Cell Biol

PubMed ID:12482977

The orphan receptor Ad4BP/SF-1 (NR5A1) is a constitutive activator, and its activity is repressed by another orphan receptor, Dax-1 (NR0B1). In the present study, we investigated the molecular mechanisms underlying this repression by Dax-1. Yeast two-hybrid and transient-transfection assays confirmed the necessity of three LXXLL-related motifs in Dax-1 for interaction ... More

Translational regulation of prostaglandin endoperoxide H synthase-1 mRNA in megakaryocytic MEG-01 cells. Specific protein binding to a conserved 20-nucleotide CIS element in the 3'-untranslated region.

Authors:Duquette M, Laneuville O

Journal:J Biol Chem

PubMed ID:12237309

Prostaglandin endoperoxide H synthase-1 (PGHS-1) is an abundant enzyme in platelets, where it plays a key role in the cascade of prostanoid formation. In platelets, the primary site of PGHS-1 synthesis is in precursor megakaryocytic cells. We have previously shown that in megakaryocytic MEG-01 cells, TPA induces an increase of ... More

Type I collagen is thermally unstable at body temperature.

Authors:Leikina E, Mertts MV, Kuznetsova N, Leikin S

Journal:Proc Natl Acad Sci U S A

PubMed ID:11805290

Molecular characterization of Saccharomyces cerevisiae TFIID.

Authors:Sanders SL, Garbett KA, Weil PA

Journal:Mol Cell Biol

PubMed ID:12138208

'We previously defined Saccharomyces cerevisiae TFIID as a 15-subunit complex comprised of the TATA binding protein (TBP) and 14 distinct TBP-associated factors (TAFs). In this report we give a detailed biochemical characterization of this general transcription factor. We have shown that yeast TFIID efficiently mediates both basal and activator-dependent transcription ... More

Mechanism of calcium-independent synaptotagmin binding to target SNAREs.

Authors:Rickman C, Davletov B

Journal:J Biol Chem

PubMed ID:12496268

'Synaptic vesicle exocytosis requires three SNARE (soluble N-ethylmaleimide-sensitive-factor attachment protein receptor) proteins: syntaxin and SNAP-25 on the plasma membrane (t-SNAREs) and synaptobrevin/VAMP on the synaptic vesicles (v-SNARE). Vesicular synaptotagmin 1 is essential for fast synchronous SNARE-mediated exocytosis and interacts with the SNAREs in brain material. To uncover the step at ... More

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