FM™ 4-64 Farbstoff (<i>N</i>-(3-Triethylammoniumpropyl)-4-(6-(4-(Diethylamino) Phenyl) Hexatrienyl) Pyridiniumdibromid)

Zitierungen und Referenzen (319)

Invitrogen™

FM™ 4-64 Farbstoff (N-(3-Triethylammoniumpropyl)-4-(6-(4-(Diethylamino) Phenyl) Hexatrienyl) Pyridiniumdibromid)

Name: FM™ 4-64 Farbstoff ( N -(3-Triethylammoniumpropyl)-4-(6-(4-(Diethylamino) Phenyl) Hexatrienyl) Pyridiniumdibromid)
SKU: T13320
Price: 868.65 EUR

Der Styrilfarbstoff FM 4-64 ermöglicht nachweislich das selektive Anfärben der Vakuolenmembranen von Hefen mit roter Fluoreszenz (Anregungs-/Emissionsmaxima ∼ 515/640 nm).Weitere Informationen

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Katalognummer	Menge
T13320	10 × 100 μg
T3166	1mg

2 Optionen

Katalognummer T13320

Preis (EUR)

868,65

온라인 행사

924,00

Ersparnis 55,35 (6%)

10 x 100 µg

Menge:

10 × 100 μg

Preis (EUR)

868,65

온라인 행사

924,00

Ersparnis 55,35 (6%)

10 x 100 µg

Der Styrilfarbstoff FM 4-64 ermöglicht nachweislich das selektive Anfärben der Vakuolenmembranen von Hefen mit roter Fluoreszenz (Anregungs-/Emissionsmaxima ∼ 515/640 nm). Der lipophile Farbstoff ist ein wichtiges Werkzeug zur Visualisierung der Morphologie und Dynamik vakuolärer Organellen, zur Untersuchung des endozytotischen Weges und für das Screening und die Charakterisierung von Hefe-Endozytose-Mutanten. FM 4-64 ist auch in Spezialverpackungen mit 10 Fläschchen mit je 100 μg (T-13320) erhältlich.

Nur für Forschungszwecke. Nicht zur Verwendung bei diagnostischen Verfahren.

Specifications

FarbeRot

NachweisverfahrenFluoreszent

Zur Verwendung mit (Geräte)Fluoreszenzmikroskop

ProduktlinieFM

Menge10 × 100 μg

VersandbedingungRaumtemperatur

MarkertypFluorescent Dye

ProdukttypFarbstoff

SubCellular LocalizationPlasmamembran

Unit Size10 x 100 µg

Inhalt und Lagerung

Lagerung bei Raumtemperatur und vor Licht geschützt

Häufig gestellte Fragen (FAQ)

I want to study endosomes trafficking using FM 4-64. Will the label be retained after fixation? And can I label already-fixed cells?

No. For that you would need the FM 4-64FX version. The non-FX version will not be retained upon fixation, leading to loss of much of the stain and an increase in background. The FX version will be retained using an aldehyde-based fixative. Cells that are already fixed will be stained throughout the cell and the signal will not be localized; it is recommended to stain live cells and then fix.

Find additional tips, troubleshooting help, and resources within our Cell Analysis Support Center.

Zitierungen und Referenzen (319)

Zitierungen und Referenzen

Abstract

Apg9p/Cvt7p is an integral membrane protein required for transport vesicle formation in the Cvt and autophagy pathways.

Authors:Noda T,Kim J,Huang WP,Baba M,Tokunaga C,Ohsumi Y,Klionsky DJ

Journal:The Journal of cell biology

PubMed ID:10662773

In nutrient-rich, vegetative conditions, the yeast Saccharomyces cerevisiae transports a resident protease, aminopeptidase I (API), to the vacuole by the cytoplasm to vacuole targeting (Cvt) pathway, thus contributing to the degradative capacity of this organelle. When cells subsequently encounter starvation conditions, the machinery that recruited precursor API (prAPI) also sequesters ... More

Glutamate induces the rapid formation of spine head protrusions in hippocampal slice cultures.

Authors:Richards DA,Mateos JM,Hugel S,de Paola V,Caroni P,Gähwiler BH,McKinney RA

Journal:Proceedings of the National Academy of Sciences of the United States of America

PubMed ID:15831587

Synaptic plasticity at neuronal connections has been well characterized functionally by using electrophysiological approaches, but the structural basis for this phenomenon remains controversial. We have studied the dynamic interactions between presynaptic and postsynaptic structures labeled with FM 4-64 and a membrane-targeted GFP, respectively, in hippocampal slices. Under conditions of reduced ... More

Fab1p is essential for PtdIns(3)P 5-kinase activity and the maintenance of vacuolar size and membrane homeostasis.

Authors:Gary JD,Wurmser AE,Bonangelino CJ,Weisman LS,Emr SD

Journal:The Journal of cell biology

PubMed ID:9763421

The Saccharomyces cerevisiae FAB1 gene encodes a 257-kD protein that contains a cysteine-rich RING-FYVE domain at its NH2-terminus and a kinase domain at its COOH terminus. Based on its sequence, Fab1p was initially proposed to function as a phosphatidylinositol 4-phosphate (PtdIns(4)P) 5-kinase (). Additional sequence analysis of the Fab1p kinase ... More

Slow spontaneous secretion from single large dense-core vesicles monitored in neuroendocrine cells.

Authors:Stenovec M, Kreft M, Poberaj I, Betz WJ, Zorec R

Journal:FASEB J

PubMed ID:15180959

Hormones are released from cells by passing through an exocytotic pore that forms after vesicle and plasma membrane fusion. In stimulated exocytosis vesicle content is discharged swiftly. Although rapid vesicle discharge has also been proposed to mediate basal secretion, this has not been studied directly. We investigated basal hormone release ... More

Traffic-independent function of the Sar1p/COPII machinery in proteasomal sorting of the cystic fibrosis transmembrane conductance regulator.

Authors:Fu L, Sztul E

Journal:J Cell Biol

PubMed ID:12538638

'Newly synthesized proteins that do not fold correctly in the ER are targeted for ER-associated protein degradation (ERAD) through distinct sorting mechanisms; soluble ERAD substrates require ER-Golgi transport and retrieval for degradation, whereas transmembrane ERAD substrates are retained in the ER. Retained transmembrane proteins are often sequestered into specialized ER ... More

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