Tetramethylrhodamin, Ethylester, Perchlorat (TMRE)

Zitierungen und Referenzen (159)

Invitrogen™

Tetramethylrhodamin, Ethylester, Perchlorat (TMRE)

Tetramethylrhodamin-Ethylester (TMRE) ist ein zelldurchlässiger, kationischer, rot-orange fluoreszierender Farbstoff, der in aktiven Mitochondrien leicht angereichert wird.Weitere Informationen

Katalognummer	Menge
T669	25 mg

Katalognummer T669

Preis (EUR)

377,65

Online Exclusive

406,00

Ersparnis 28,35 (7%)

25 mg

Menge:

25 mg

Preis (EUR)

377,65

Online Exclusive

406,00

Ersparnis 28,35 (7%)

25 mg

Tetramethylrhodamin-Ethylester (TMRE) ist ein zelldurchlässiger, kationischer, rot-orange fluoreszierender Farbstoff, der in aktiven Mitochondrien leicht angereichert wird.

Nur für Forschungszwecke. Nicht zur Verwendung bei diagnostischen Verfahren.

Specifications

NachweisverfahrenFluoreszenz

Anregung/Emission549/574 nm

Molekulargewicht514.96

Menge25 mg

VersandbedingungRaumtemperatur

Subzelluläre LokalisationMitochondrien

FarbeRot-Orange

Zur Verwendung mit (Geräte)Fluoreszenzmikroskop

ProdukttypTMRE

Unit Size25 mg

Inhalt und Lagerung

Bei -5 bis -30 °C lagern und vor Licht schützen.

Häufig gestellte Fragen (FAQ)

I am seeing high background outside of my neuronal cells when using membrane potential indicators. What can I do to reduce background?

If you use our FluoVolt Membrane Potential Kit (Cat. No. F10488), the kit provides a background suppressor to reduce this problem. For other indicators, consider the use of BackDrop Background Suppressor (Cat no. R37603, B10511, and B10512).

Find additional tips, troubleshooting help, and resources within our Cell Analysis Support Center.

What is the difference between fast and slow-response membrane potential probes?

Molecules that change their structure in response to the surrounding electric field can function as fast-response probes for the detection of transient (millisecond) potential changes. Slow-response dyes function by entering depolarized cells and binding to proteins or membranes. Increased depolarization results in additional dye influx and an increase in fluorescence, while hyperpolarization is indicated by a decrease in fluorescence. Fast-response probes are commonly used to image electrical activity from intact heart tissues or measure membrane potential changes in response to pharmacological stimuli. Slow-responding probes are often used to explore mitochondrial function and cell viability.

Find additional tips, troubleshooting help, and resources within our Cell Analysis Support Center.

What type of membrane potential indicators do you offer and how should I choose one for my experiment?

A membrane potential indicator selection guide can be found here (https://www.thermofisher.com/us/en/home/life-science/cell-analysis/cell-viability-and-regulation/ion-indicators/membrane-potential-indicators.html).

Find additional tips, troubleshooting help, and resources within our Cell Analysis Support Center.

Zitierungen und Referenzen (159)

Zitierungen und Referenzen

Abstract

A hyperfused mitochondrial state achieved at G1-S regulates cyclin E buildup and entry into S phase.

Authors:Mitra K, Wunder C, Roysam B, Lin G, Lippincott-Schwartz J,

Journal:Proc Natl Acad Sci U S A

PubMed ID:19617534

Mitochondria undergo fission-fusion events that render these organelles highly dynamic in cells. We report a relationship between mitochondrial form and cell cycle control at the G(1)-S boundary. Mitochondria convert from isolated, fragmented elements into a hyperfused, giant network at G(1)-S transition. The network is electrically continuous and has greater ATP ... More

Antiapoptotic effect of nicorandil mediated by mitochondrial atp-sensitive potassium channels in cultured cardiac myocytes.

Authors:Akao M, Teshima Y, MarbÃ¡n E

Journal:J Am Coll Cardiol

PubMed ID:12204514

OBJECTIVES: We examined whether nicorandil, a clinically useful drug for the treatment of ischemic syndromes, inhibits myocardial apoptosis. BACKGROUND: Nicorandil has been reported to have a cardioprotective action through activation of mitochondrial ATP-sensitive potassium (mitoK(ATP)) channels. Based on our recent observation that mitoK(ATP) channel activation has a remarkable antiapoptotic effect ... More

Synaptic mitochondria are more susceptible to Ca2+overload than nonsynaptic mitochondria.

Authors:Brown MR, Sullivan PG, Geddes JW

Journal:J Biol Chem

PubMed ID:16517608

'Mitochondria in nerve terminals are subjected to extensive Ca2+ fluxes and high energy demands, but the extent to which the synaptic mitochondria buffer Ca2+ is unclear. In this study, we identified a difference in the Ca2+ clearance ability of nonsynaptic versus synaptic mitochondrial populations enriched from rat cerebral cortex. Mitochondria ... More

Age-related macular degeneration. The lipofusion component N-retinyl-N-retinylidene ethanolamine detaches proapoptotic proteins from mitochondria and induces apoptosis in mammalian retinal pigment epithelial cells.

Authors:Suter M, Remé C, Grimm C, Wenzel A, Jäättela M, Esser P, Kociok N, Leist M, Richter C

Journal:J Biol Chem

PubMed ID:11006290

'10-20% of individuals over the age of 65 suffer from age-related macular degeneration (AMD), the leading cause of severe visual impairment in humans living in developed countries. The pathogenesis of this complex disease is poorly understood, and no efficient therapy or prevention exists to date. A precondition for AMD appears ... More

VDAC-dependent permeabilization of the outer mitochondrial membrane by superoxide induces rapid and massive cytochrome c release.

Authors:Madesh M, Hajnóczky G

Journal:J Cell Biol

PubMed ID:11739410

'Enhanced formation of reactive oxygen species (ROS), superoxide (O2*-), and hydrogen peroxide (H2O2) may result in either apoptosis or other forms of cell death. Here, we studied the mechanisms underlying activation of the apoptotic machinery by ROS. Exposure of permeabilized HepG2 cells to O2*- elicited rapid and massive cytochrome c ... More

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