Zitierungen und Referenzen (4)

Invitrogen™

pLenti6/V5-DEST™ Gateway™ Vector

Katalognummer	Menge
V49610	6μ g

Katalognummer V49610

Preis (EUR)

3.645,00

Menge:

Preis (EUR)

3.645,00

Der pLenti6⁄V5-DEST™ Gateway™ Vektor ist ein Gateway™ angepasster ViraPower™ Lentiviraler Expressionsvektor für die lentivirale Expression eines Zielgens in sich teilenden und nicht teilenden Säugetierzellen. Der Vektor verfügt über den CMV-Promotor zur Förderung der konstitutiven Expression des Zielgens und den PGK-Promotor zur Förderung der Expression des stabilen Blasticidin-Auswahlmarkers.

Vorteile
• Lentivirus-basierte Expression eines Zielgens in sich teilenden und nicht teilenden Säugetierzellen

Wichtige Merkmale
• Flexible und vielseitige Gateway™ Rekombinationskloniertechnologie
• Konstitutiv hohe Expression mit CMV-Promotor
• Blasticidin-Selektionsmarker für eine stabile Selektion
• C-terminales V5-Tag für schnelle Nachweise

Das Kit enthält
• pLenti6⁄V5-DEST™ Gateway™ Vektor
• One Shot™ Stbl3™ Chemisch kompetente E. coli (C7373-03)

Verwandte SKU
• pLenti6⁄UBC⁄V5-DEST™ Gateway™ Vektor (V49910)
• pLenti4⁄V5-DEST™ Gateway™ Vektor (V49810)
• pLlenti6⁄V5 Directional TOPO™ Cloning Kit (K4955-10)
• ViraPower™ Lentiviral Directional TOPO™ Expressionskit (K495000)
• ViraPower™ Lentiviral Gateway™ Expressionskit (K4960-00)
• ViraPower™ HiPerform™ Lentiviral Gateway™ Expressionskit (K5330-00)

Nur für Forschungszwecke. Nicht für therapeutische oder diagnostische Zwecke vorgesehen.

Nur für Forschungszwecke. Nicht zur Verwendung bei diagnostischen Verfahren.

Specifications

Konstitutives oder induktives SystemKonstitutiv

LiefertypLentiviral

Zur Verwendung mit (Anwendung)Virale Expression

ProdukttypLentivirus-Expressionsvektor

Menge6μ g

Selektionsmittel (eukaryotisch)Blasticidin

VektorpDEST, pLenti

KlonierungsmethodeGateway™

ProduktlinieGateway, ViraPower

PromoterCMV

ProteinmarkierungV5-Epitop-Tag

Unit Size6 µg

Inhalt und Lagerung

Lagerung von ViraPower™ Lentiviralen Gateway™ Vektoren bei –20 °C, enthält:

pLenti6⁄V5-DEST™: 6 µg (40 µl 150 ng⁄µl-Vektor in 10 mM
Tris-HCl, 1 mM EDTA, pH 8,0)

pLenti6⁄V5-GW⁄lacZ-Kontrolle: 10 µg
(20 µl 0,5 µg⁄µl-Vektor in 10 mM
Tris-HCl, 1 mM EDTA, pH 8,0)

In One Shot™ Stbl3™ Chemisch kompetente E. coli bei –80 °C lagern

Häufig gestellte Fragen (FAQ)

Can I perform the single-step protocol for the BP/LR Clonase reaction using BP Clonase enzyme and LR Clonase enzyme instead of BP Clonase II enzyme and LR Clonase II enzyme?

In the single-step protocol for the BP/LR Clonase reaction, we would not recommend substituting the BP Clonase II/LR Clonase II enzymes with BP Clonase /LR Clonase enzymes as this would result in very low recombination efficiency.

Do you have a recommended single-step protocol for BP/LR recombination?

Yes, we have come up with a single-step protocol for BP/LR Clonase reaction (http://www.thermofisher.com/us/en/home/life-science/cloning/gateway-cloning.html#1), where DNA fragments can be cloned into Destination vectors in a single step reaction, allowing you to save time and money.

How can I move my gene of interest from a Gateway-adapted expression clone to a new Destination vector as I have lost the entry clone?

We would recommend performing a BP reaction with a Donor vector in order to obtain an entry clone. This entry clone can then be used in an LR reaction with the Destination vector to obtain the new expression clone.

Can I purchase the 5X LR Clonase buffer or 5X BP Clonase buffer separately?

We do not offer the 5X LR Clonase buffer and 5X BP Clonase buffer as standalone products. They are available as part of the enzyme kits.

Do you offer Gateway vectors for expression in plants?

We do not offer any Gateway vectors for expression in plants.

View all pLenti6/V5-DEST™ Gateway™ Vector FAQs

Zitierungen und Referenzen (4)

Zitierungen und Referenzen

Abstract

The membrane anchor R7BP controls the proteolytic stability of the striatal specific RGS protein, RGS9-2.

Authors:Anderson GR, Semenov A, Song JH, Martemyanov KA,

Journal:J Biol Chem

PubMed ID:17158100

'A member of the RGS (regulators of G protein signaling) family, RGS9-2 is a critical regulator of G protein signaling pathways that control locomotion and reward signaling in the brain. RGS9-2 is specifically expressed in striatal neurons where it forms complexes with its newly discovered partner, R7BP (R7 family binding ... More

West Nile virus discriminates between DC-SIGN and DC-SIGNR for cellular attachment and infection.

Authors:Davis CW, Nguyen HY, Hanna SL, Sánchez MD, Doms RW, Pierson TC,

Journal:J Virol

PubMed ID:16415006

'The C-type lectins DC-SIGN and DC-SIGNR bind mannose-rich glycans with high affinity. In vitro, cells expressing these attachment factors efficiently capture, and are infected by, a diverse array of appropriately glycosylated pathogens, including dengue virus. In this study, we investigated whether these lectins could enhance cellular infection by West Nile ... More

Tumorigenesis suppressor Pdcd4 down-regulates mitogen-activated protein kinase kinase kinase kinase 1 expression to suppress colon carcinoma cell invasion.

Authors:Yang HS, Matthews CP, Clair T, Wang Q, Baker AR, Li CC, Tan TH, Colburn NH,

Journal:Mol Cell Biol

PubMed ID:16449643

Programmed cell death 4 (Pdcd4) suppresses neoplastic transformation by inhibiting the activation of c-Jun and consequently AP-1-dependent transcription. We report that Pdcd4 blocks c-Jun activation by inhibiting the expression of mitogen-activated protein kinase kinase kinase kinase 1 (MAP4K1)/hematopoietic progenitor kinase 1, a kinase upstream of Jun N-terminal kinase (JNK). cDNA ... More

Isolation of cell lines that show novel, murine leukemia virus-specific blocks to early steps of retroviral replication.

Authors:Bruce JW, Bradley KA, Ahlquist P, Young JA,

Journal:J Virol

PubMed ID:16188999

In order to identify cellular proteins required for early stages of retroviral replication, a high volume screening with mammalian somatic cells was performed. Ten pools of chemically mutagenized Chinese hamster ovary (CHO-K1) cells were challenged with a murine leukemia virus (MLV) vector pseudotyped with the vesicular stomatitis virus glycoprotein (VSV-G), ... More