MEM α, nucleosides
MEM α, nucleosides
Citations & References (9)
Gibco™

MEM α, nucleosides

MEM α (Minimum Essential Medium α) is widely used for mammalian cell culture as well as selection for transfected DHFR-negativeRead more
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Catalog NumberQuantity
12571063500 mL
22571020
also known as 22571-020
500 mL
22571038
also known as 22571-038
10 x 500 mL
Catalog number 12571063
Price (EUR)
124,65
Online Exclusive
137,00
Save 12,35 (9%)
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Quantity:
500 mL
Customize this product
Price (EUR)
124,65
Online Exclusive
137,00
Save 12,35 (9%)
Each
Add to cart

MEM α (Minimum Essential Medium α) is widely used for mammalian cell culture as well as selection for transfected DHFR-negative cells. MEM α can be used with a variety of suspension and adherent mammalian cells, including keratinocytes, primary rat astrocytes, and human melanoma cells. We offer a variety of MEM α modifications for a range of cell culture applications. Find the right formulation using the media selector tool.


This MEM α is modified as follows:
With
• Ribonucleosides
• Deoxyribonucleosides
• Phenol Red
• L-glutamine


The complete formulation is available.

Using MEM α
MEM α is a modification of Minimum Essential Medium (MEM) that contains non-essential amino acids, sodium pyruvate, lipoic acid, vitamin B12, biotin, and ascorbic acid. MEM α is available without nucleosides for use as a selection medium for DG44 and other DHFR-negative cells. This product is made with Earle’s salts. MEM α contains no proteins, lipids, or growth factors. Therefore, MEM α requires supplementation, commonly with 10% Fetal Bovine Serum (FBS). MEM α uses a sodium bicarbonate buffer system (2.2 g/L), and therefore requires a 5–10% CO2 environment to maintain physiological pH.

For Research Use or Further Manufacturing. Not for diagnostic use or direct administration into humans or animals.
Specifications
Cell LineHeLa, BHK-21, 293, HEP-2, HT-1080, MCF-7, and fibroblasts
Cell TypePrimary Rat Astrocytes
Concentration1 X
Manufacturing QualitycGMP-compliant under the ISO 13485 standard
Product LineGibco
Product TypeMEM α (Minimum Essential Medium α)
Quantity500 mL
Shelf Life12 Months From Date of Manufacture
Shipping ConditionRoom Temperature
ClassificationAnimal Origin-free
FormLiquid
Serum LevelStandard Serum Supplementation
SterilitySterile-filtered
Sterilization MethodSterile-filtered
With AdditivesLow Glucose, Glutamine, Phenol Red, Sodium Pyruvate, Ribonucleosides, Deoxyribonucleosides
Without AdditivesNo HEPES
Unit SizeEach
Contents & Storage
Storage conditions: 2-8° C. Protect from light
Shipping conditions: Ambient
Shelf life: 12 months from date of manufacture

Frequently asked questions (FAQs)

Do you offer an alternative for MEM α, nucleosides, no ascorbic acid (Cat. No. A1049001)

Unfortunately, we do not carry another MEM α formulation without ascorbic acid. The closest alternative would be MEM α, nucleosides (Cat. No. 12571063). However, this formulation contains ascorbic acid. The formulation can be found here:

Media Formulation: MEM alpha, nucleosides

Please use the link below to the Gibco Cell Culture Media Formulation Tool, which can be very helpful when comparing our different formulations.

Gibco Cell Culture Media Formulation Tool

Find additional tips, troubleshooting help, and resources within our Cell Culture Support Center.

What is the difference between MEM α, nucleosides and MEM α, no nucleosides?

Our MEM α, nucleosides is widely used for mammalian cell culture as well as selection for transfected DHFR-negative cells.
Our MEM α without nucleosides can be used a selection medium for DG44 and other DHFR-negative cells (DHFR = dihydrofolate reductase).
DHFR-negative cells are unable to grow unless the culture medium is supplemented with essential metabolites, including hypoxanthine and thymidine.

Where can I find the osmolality for MEM Medium?

The osmolality is listed in the COA for the particular lot number of the medium.

Find additional tips, troubleshooting help, and resources within our Cell Culture Support Center.

How long can I keep my media after supplementing with serum?

Generally speaking, media can be used for up to three weeks after supplementation with serum. There are no formal studies to support this, but it is the rule of thumb used by our scientists.

Find additional tips, troubleshooting help, and resources within our Mammalian Cell Culture Basics Support Center.

My medium was shipped at room temperature but it is supposed to be stored refrigerated. Is it okay?

We routinely ship media that require long-term storage in the refrigerator at room temperature. We have done studies on representative media formulations to show that media can be at room temperature for up to a week without a problem.

Find additional tips, troubleshooting help, and resources within our Mammalian Cell Culture Basics Support Center.

View all MEM α, nucleosides, 500 mL FAQs

Citations & References (9)

Citations & References
Abstract
Establishment of induced pluripotent stem cells from aged mice using bone marrow-derived myeloid cells.
Authors:Cheng Z, Ito S, Nishio N, Xiao H, Zhang R, Suzuki H, Okawa Y, Murohara T, Isobe K
Journal:J Mol Cell Biol
PubMed ID:21228011
'If induced pluripotent stem (iPS) cells are to be used to treat damaged tissues or repair organs in elderly patients, it will be necessary to establish iPS cells from their tissues. To determine the feasibility of using this technology with elderly patients, we asked if it was indeed possible to ... More
A high-throughput automated platform for the development of manufacturing cell lines for protein therapeutics.
Authors:Shi S, Condon RG, Deng L, Saunders J, Hung F, Tsao YS, Liu Z
Journal:J Vis Exp
PubMed ID:21968840
'The fast-growing biopharmaceutical industry demands speedy development of highly efficient and reliable production systems to meet the increasing requirement for drug supplies. The generation of production cell lines has traditionally involved manual operations that are labor-intensive, low-throughput and vulnerable to human errors. We report here an integrated high-throughput and automated ... More
Fragmentation and dispersal of the pericentriolar Golgi complex is required for entry into mitosis in mammalian cells.
Authors: Sütterlin Christine; Hsu Pattie; Mallabiabarrena Arrate; Malhotra Vivek;
Journal:Cell
PubMed ID:12015985
The pericentriolar Golgi stacks are fragmented and found dispersed in mitotic mammalian cells. Addition of an antibody to the Golgi-associated protein GRASP65 inhibited Golgi fragmentation by mitotic cytosol in permeabilized cells. Microinjecting this antibody or the C-terminal fragment of GRASP65, which contains the antibody binding site, into normal rat kidney ... More
Lack of Fucose on Human IgG1 N-Linked Oligosaccharide Improves Binding to Human Fcgamma RIII and Antibody-dependent Cellular Toxicity.
Authors: Shields Robert L; Lai Jadine; Keck Rodney; O'Connell Lori Y; Hong Kyu; Meng Y Gloria; Weikert Stefanie H A; Presta Leonard G;
Journal:J Biol Chem
PubMed ID:11986321
Lec13 cells, a variant Chinese hamster ovary cell line, were used to produce human IgG1 that were deficient in fucose attached to the Asn(297)-linked carbohydrate but were otherwise similar to that found in IgG1 produced in normal Chinese hamster ovary cell lines and from human serum. Lack of fucose on ... More
Beta -catenin is essential and sufficient for skeletal myogenesis in p19 cells.
Authors: Petropoulos Helen; Skerjanc Ilona S;
Journal:J Biol Chem
PubMed ID:11856745
Wnt1 and Wnt3a are signaling factors known to play a role in the induction of myogenesis in the myotome of the differentiating somite. Both factors may transduce their signal by a conserved pathway that leads to transcriptional regulation by beta-catenin/Lef1. beta-Catenin and Lef1 are found in the myotome prior to ... More
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