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Citations & References (16)
Gibco™
Opti-MEM™ I Reduced Serum Medium
Opti-MEM™ I Reduced-Serum Medium is an improved Minimal Essential Medium (MEM) that allows for a reduction of Fetal Bovine SerumRead more
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| Catalog Number | Quantity |
|---|---|
| 31985062 | 100 mL |
| 31985070 | 500 mL |
Catalog number 31985062
Price (EUR)
14,94
Each
Quantity:
100 mL
Price (EUR)
14,94
Each
Opti-MEM™ I Reduced-Serum Medium is an improved Minimal Essential Medium (MEM) that allows for a reduction of Fetal Bovine Serum supplementation by at least 50% with no change in growth rate or morphology. Opti-MEM™ I medium is also recommended for use with cationic lipid transfection reagents, such as Lipofectamine™ reagent. Opti-MEM™ I medium can be used with a variety of suspension and adherent mammalian cells, including Sp2, AE-1, CHO, BHK-21, HEK, and primary fibroblasts. We offer a variety of Opti-MEM™ I modifications for a range of cell culture applications.
This Opti-MEM™ I is modified as follows:
| With |
| • L-glutamine |
| • Phenol Red |
The complete formulation is confidential. For more information, please contact Technical Services.
Using Opti-MEM™ I Medium
Opti-MEM™ I Reduced-Serum Medium is a unique medium that contains insulin, transferrin, hypoxanthine, thymidine, and trace elements. These additional components allow for a reduction in serum supplementation by at least 50%. Opti-MEM™ I medium uses a sodium bicarbonate buffer system (2.4 g/L), and therefore requires a 5–10% CO2 environment to maintain physiological pH.
For Research Use or Further Manufacturing. Not for diagnostic use or direct administration into humans or animals.
Specifications
Cell LineSp2, AE-1, CHO, BHK-21, and HEK
Cell TypePrimary Fibroblasts
Concentration1 X
Manufacturing QualitycGMP-compliant under the ISO 13485 standard
Product LineGibco, Opti-MEM
Product TypeOpti-MEM I
Quantity100 mL
Shelf Life18 Months From Date of Manufacture
Shipping ConditionRoom Temperature
ClassificationHuman Origin
FormLiquid
SterilitySterile-filtered
Sterilization MethodSterile-filtered
With AdditivesGlutamine, Phenol Red
Unit SizeEach
Contents & Storage
Store at 2–8°C and protect from light.
Frequently asked questions (FAQs)
What should I use to dilute my RNAi duplexes and Lipofectamine RNAiMAX before complexing?
Do you offer an alternative for Opti-MEM I Reduced Serum Medium (Cat. No. 31985070)?
My OpitMEM Reduced Serum Medium (Cat. No. 31985070) was accidentally frozen. Can I still use it?
Is there any other difference between Opti-MEM I Reduced Serum Medium and Opti-MEM I Reduced Serum Medium, no phenol red besides the presence/absence of phenol red?
Where can I find the osmolality for my lot of Opti-MEM I Reduced Serum Medium?
Citations & References (16)
Citations & References
Abstract
Identification of a novel redox-sensitive gene, Id3, which mediates angiotensin II-induced cell growth.
Journal:Circulation
PubMed ID:12021231
BACKGROUND: Reactive oxygen species, such as superoxide (O(2)(-)), are involved in the abnormal growth of various cell types. Angiotensin II (Ang II) is one of the most potent inducers of oxidative stress in the vasculature. The molecular events involved in Ang II-induced proliferation of vascular smooth muscle cells (VSMCs) are
Regulation of cortical dendrite development by Slit-Robo interactions.
Journal:Neuron
PubMed ID:11779471
Slit proteins have previously been shown to regulate axon guidance, branching, and neural migration. Here we report that, in addition to acting as a chemorepellant for cortical axons, Slit1 regulates dendritic development. Slit1 is expressed in the developing cortex, and exposure to Slit1 leads to increased dendritic growth and branching.
Vinexin beta Regulates the Anchorage Dependence of ERK2 Activation Stimulated by Epidermal Growth Factor.
Journal:J Biol Chem
PubMed ID:11825889
'ERK is activated by soluble growth factors in adherent cells. However, activation of ERK is barely detectable and not sufficient for cell proliferation in non-adherent cells. Here, we show that exogenous expression of vinexin beta, a novel focal adhesion protein, allows anchorage-independent ERK2 activation stimulated by epidermal growth factor. In
Hypoxia induces cyclooxygenase-2 via the NF-kappaB p65 transcription factor in human vascular endothelial cells.
Journal:J Biol Chem
PubMed ID:8995303
'The inducible cyclooxygenase, COX-2, has been associated with vascular inflammation and cellular proliferation. We have discovered that hypoxia increases expression of the COX-2 gene in human vascular endothelial cells in culture independent of other stimuli. Western analysis of human umbilical vein endothelial cells (HUVEC) revealed a greater than 4-fold induction
The proximal promoter of the human transglutaminase 3 gene. Stratified squamous epithelial-specific expression in cultured cells is mediated by binding of Sp1 and ets transcription factors to a proximal promoter element.
Journal:J Biol Chem
PubMed ID:8626812
'The transglutaminase 3 enzyme is expressed during the late stages of the terminal differentiation of the epidermis and in certain cell types of the hair follicle. The enzyme is thought to be critically involved in the cross-linking of structural proteins and in the formation of the cornified cell envelope, thereby