Échelle de protéines à plage élevée et multicolore Spectra™
Thermo Scientific™

Échelle de protéines à plage élevée et multicolore Spectra™

L’échelle de protéines à plage élevée multicolore Thermo Scientific Spectra est un mélange de huit protéines colorées en bleu, vertAfficher plus
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RéférenceQuantité
266252 x 250 μl
26625X48 x 250 μL
Référence 26625
Prix (EUR)
246,65
Online Exclusive
284,00
Économisez 37,35 (13%)
Each
Quantité:
2 x 250 μl
Grand volume ou format personnalisé
Prix (EUR)
246,65
Online Exclusive
284,00
Économisez 37,35 (13%)
Each
L’échelle de protéines à plage élevée multicolore Thermo Scientific Spectra est un mélange de huit protéines colorées en bleu, vert et orange (40 à 300 kDa) à utiliser comme étalons de taille pour les protéines à poids moléculaire élevé lors de l’électrophorèse sur gel et des transferts Western. L’échelle de protéines est prête à l’emploi pour le chargement direct sur les gels ; nul besoin de la chauffer, de la réduire ou d’ajouter un tampon d’échantillon avant de l’utiliser.

Découvrez et comparez tous les autres étalons et échelles de protéines ›

Applications
• Surveillance de la migration des protéines lors de la SDS-PAGE
• Vérification de l’efficacité du transfert Western
• Dimensionnement approximatif des protéines sur les gels de polyacrylamide-SDS et les transferts Western
• Localisation d’une protéine d’intérêt pour l’excision à partir d’un gel préparatif non coloré

Usage exclusivement réservé à la recherche. Ne pas utiliser pour des procédures de diagnostic.

Spécifications
Méthode de détectionColorimétrique, fluorescence dans le proche IR (700 nm), fluorescence RVB (555 nm)
À utiliser avec (application)Tris-acetate gels
Compatibilité du gelGels Bolt™ Bis-Tris Plus, gels Novex™ Tricine, gels Novex™ Tris-Glycine, gels NuPAGE™ Bis-Tris, gels NuPAGE™ Tris-Acétate, gels SDS-PAGE
Poids moléculaire300, 250, 180, 130, 100, 70, 50, 40 kDa
Quantité2 x 250 μl
Prêt à chargerOui
Applications recommandéesGels Tris-Acétate
Conditions d’expéditionHomologué pour des expéditions sur de la glace carbonique ou mouillée
Number of Markers8
Gamme de produitsSpectra
Type de produitÉchelle de protéines
Plage de dimensions40 à 300 kDa
Stain Type3 couleurs : bleu, orange, vert
System TypeTransfert Western, SDS-PAGE
Unit SizeEach
Contenu et stockage
Contenu : deux flacons de 250 μl chacun

Tampon de stockage : 62,5 mM de Tris-H3PO4 (pH 7,5 à 25°C), 1 mM d’EDTA, 2 % de SDS, 10 mM de DTT, 1 mM de NaN3 et 33 % de glycérol

Stockage : Dès réception, conserver à -20°C

Foire aux questions (FAQ)

Why do Thermo Scientific prestained protein ladders not show the real protein sizes?

Coupling of chromophores to proteins affects the apparent molecular weight of proteins in SDS-PAGE relative to unstained standards. The apparent molecular weight of prestained protein standards is calibrated in the classical TRIS glycine-SDS Laemmli system, however prestained proteins may have different mobility in other electrophoresis buffer and gel systems. It should also be noted that the sizing of proteins by gel electrophoresis does not give an exact value and depends on the protein sequence and post-modification.

Find additional tips, troubleshooting help, and resources within our Protein Assays and Analysis Support Center.

The upper bands of the ladder are missing. What could be the reason?

The upper bands of the ladder may be degraded by proteases. Ladder, gel, buffer, pipettes, pipette tips, or equipment can be contaminated by proteases during usage. A general recommendation would be to avoid working with proteases in the same room. We would recommend preparing fresh solutions, cleaning the equipment, and using clean pipettes and tips. If the ladder itself is contaminated, please use a new tube of the ladder.

Find additional tips, troubleshooting help, and resources within our Protein Assays and Analysis Support Center.

Do the proteins in Thermo Scientific protein ladders have a His-Tag or would otherwise react with an anti-His-Tag antibody?

No, proteins in Thermo Scientific protein ladders are not His tagged. However, non-specific interaction between the ladder proteins and primary or secondary antibodies is possible and some His-Tag detection systems, such as Thermo Scientific 6xHis Protein Tag Stain Reagent Kit, show non-specific interaction. The protein ladder bands are more readily detected when using high antibody concentrations. The non-specific cross-reactivity is difficult to predict, it often has a different pattern dependent on the antibodies used in each individual experiment. The most general way to handle this problem would be to use lower concentrations of antibodies and to use lower amount of protein ladders. It may also be useful to leave one empty well between the ladder and the sample to overcome a possible leakage of the signal to the nearby sample lane.

Find additional tips, troubleshooting help, and resources within our Protein Assays and Analysis Support Center.

Can Thermo Scientific protein ladders be detected by Strep-Tactin conjugates?

PageRuler Unstained protein ladders can be detected directly on Western blots by using Strep-Tactin conjugates or an antibody against the Strep-tag II sequence. All PageRuler and Spectra ladder proteins contain an integral Strep-tag II sequence, however the prestained ladders cannot be detected by Strep-Tactin conjugates.

Find additional tips, troubleshooting help, and resources within our Protein Assays and Analysis Support Center.

Why are the PageRuler and Spectra ladder bands detected with antibodies against eukaryotic proteins?

All PageRuler and Spectra ladder bands are recombinant prokaryotic proteins purified from E. coli cells. None of them are related to eukaryotic proteins, however this cannot exclude the possibility that the ladder proteins may possess an epitope that is cross-reactive with the antibody used.

Find additional tips, troubleshooting help, and resources within our Protein Assays and Analysis Support Center.