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Try new Gibco Insulin-Transferrin-Selenium Select supplements, an animal-origin free formulation. Available in ITS-G & ITS-X. Learn more >
Insuline-transferrine-sélénium (ITS -G) (100X)
Insuline-transferrine-sélénium (ITS -G) (100X)
Insuline-transferrine-sélénium (ITS -G) (100X)
Citations et références (4)
Gibco™

Insuline-transferrine-sélénium (ITS -G) (100X)

La solution Gibco™ Insulin-Transferrin-Selenium (ITS-G) est utilisée comme supplément du milieu basal pour réduire la quantité de sérum de veauAfficher plus
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RéférenceQuantité
4140004510 ml
Référence 41400045
Prix (EUR)
69,65
Online Exclusive
77,00
Économisez 7,35 (10%)
Each
Quantité:
10 ml
Prix (EUR)
69,65
Online Exclusive
77,00
Économisez 7,35 (10%)
Each
La solution Gibco™ Insulin-Transferrin-Selenium (ITS-G) est utilisée comme supplément du milieu basal pour réduire la quantité de sérum de veau fœtal (SVF) nécessaire à la culture des cellules. Initialement utilisé avec RPMI 1640 et le milieu minimum essentiel (MEM), la solution ITS-G est à présent couramment utilisée avec d’autres milieux de base pour traiter une vaste gamme de types cellulaires en présence de 2 à 4 % de SVF.

L’insuline favorise l’absorption du glucose et des acides aminés, la lipogenèse, le transport intracellulaire et la synthèse des protéines et des acides nucléiques. La transferrine est une protéine porteuse de fer et peut également contribuer à réduire les niveaux toxiques de radicaux libres d’oxygène et de peroxyde. Le sélénium, présent sous la forme de sélénite de sodium, est un cofacteur pour le glutathion peroxydase et d’autres protéines, et est utilisé comme agent antioxydant dans les milieux de culture.

La formulation complète est disponible.

Système de fabrication et de qualité conformes aux BPFa
Gibco™ ITS-G est fabriqué sur un site conforme aux BPFa situé à Grand Island, New York, États-Unis. Ce site est homologué par la FDA comme fabricant de dispositifs médicaux et il est certifié ISO 13485.
Usage exclusivement réservé à la recherche. Ne pas utiliser pour des procédures de diagnostic.
Spécifications
Type de celluleCellules de mammifères
Concentration100 X
Quantité10 ml
Durée de conservation18 mois
Conditions d’expéditionTempérature ambiante
FormeLiquide
Type de produitTransferrine
StérilitéStérilisation par filtration
Sans additifSans rouge de phénol
Unit SizeEach
Contenu et stockage
Conditions de stockage : 2°C à 8°C
Conditions d’expédition : température ambiante
Durée de conservation : 18 mois à compter de la date de fabrication

Foire aux questions (FAQ)

What is the buffer composition of the various Insulin-Transferrin-Selenium (ITS) (100X) and Insulin-Transferrin-Selenium (ITS) Select (100X) supplements?

All ITS and ITS Select supplements are formulated in Earle's Balanced Salt Solution (EBSS).

Find additional tips, troubleshooting help, and resources within our Cell Culture Support Center.

What is the difference between Insulin-Transferrin-Selenium (ITS) (100X) and Insulin-Transferrin-Selenium (ITS) Select (100X) supplements?

ITS Select supplements are animal origin-free (AOF) versions of the ITS supplements offered by Thermo Fisher Scientific.

ITS Select supplements use recombinant versions of both proteins in the formulation: insulin and transferrin.

All other versions of ITS, those without the Select designation, utilize recombinant insulin and a human-derived holo transferrin.

Find additional tips, troubleshooting help, and resources within our Cell Culture Support Center.

What are the individual concentrations of insulin, transferrin, and selenium in the Insulin-Transferrin-Selenium (ITS) (100X) supplements?

All ITS and ITS Select supplements have the following concentrations of their components:

Insulin: 1.0 g/L
Transferrin: 0.55 g/L
Selenium: 0.00067 g/L

Find additional tips, troubleshooting help, and resources within our Cell Culture Support Center.

Can I store aliquots of Insulin-Transferrin-Selenium (ITS) (100X) supplements at -20 degrees C without compromising the integrity of the product?

We recommend storing all ITS and ITS Select supplements at 2-8 degrees C as there is a chance that the insulin will precipitate upon freezing and thawing.

Find additional tips, troubleshooting help, and resources within our Cell Culture Support Center.

Are all Insulin-Transferrin-Selenium (ITS) (100X) supplements offered by Thermo Fisher Scientific animal origin-free?

We offer both animal origin and animal origin-free versions of our ITS supplements. The animal origin-free product versions are referred to as ITS Select supplements.

Insulin-Transferrin-Selenium (ITS-G) (100X) (Cat. No. 41400045), Insulin-Transferrin-Selenium-Ethanolamine (ITS-X) (100X) (Cat. No. 51500056), and Insulin-Transferrin-Selenium-Sodium Pyruvate (ITS-A) (100X) (Cat. No. 51300044) are not animal origin-free.

Animal origin-free product versions are available for the ITS-G and ITS-X formulations: Insulin-Transferrin-Selenium (ITS-G) Select Supplement (100X) (Cat. No. A4000046501 and A4000046502) and Insulin-Transferrin-Selenium-Ethanolamine (ITS-X) Select Supplement (100X) (Cat. No. A4000046401 and Cat. No. A4000046402.

Find additional tips, troubleshooting help, and resources within our Cell Culture Support Center.

View all Insuline-transferrine-sélénium (ITS -G) (100X) FAQs

Citations et références (4)

Citations et références
Abstract
A comparison between different human hepatocyte models reveals profound differences in net glucose production, lipid composition and metabolism in vitro.
Authors:Bonanini F,Singh M,Yang H,Kurek D,Harms AC,Mardinoglu A,Hankemeier T
Journal:Experimental cell research
PubMed ID:38499143
Protocol to generate human liver spheroids to study liver fibrosis induced by metabolic stress.
Authors:Kim HY,Lee W,Liu X,Jang H,Sakane S,Carvalho-Gontijo Weber R,Diggle K,Kerk SA,Metallo CM,Kisseleva T,Brenner DA
Journal:STAR protocols
PubMed ID:38833372
Currently, there is no effective treatment for obesity and alcohol-associated liver diseases, partially due to the lack of translational human models. Here, we present a protocol to generate 3D human liver spheroids that contain all the liver cell types and mimic “livers in a dish.” We describe strategies to induce ... More
Collective cell migration requires suppression of actomyosin at cell-cell contacts mediated by DDR1 and the cell polarity regulators Par3 and Par6.
Authors:Hidalgo-Carcedo C, Hooper S, Chaudhry SI, Williamson P, Harrington K, Leitinger B, Sahai E,
Journal:Nat Cell Biol
PubMed ID:21170030
Collective cell migration occurs in a range of contexts: cancer cells frequently invade in cohorts while retaining cell-cell junctions. Here we show that collective invasion by cancer cells depends on decreasing actomyosin contractility at sites of cell-cell contact. When actomyosin is not downregulated at cell-cell contacts, migrating cells lose cohesion. ... More
Human cardiomyocyte progenitor cells differentiate into functional mature cardiomyocytes: an in vitro model for studying human cardiac physiology and pathophysiology.
Authors:Smits AM, van Vliet P, Metz CH, Korfage T, Sluijter JP, Doevendans PA, Goumans MJ,
Journal:Nat Protoc
PubMed ID:19197267
To date, there is no suitable in vitro model to study human adult cardiac cell biology. Although embryonic stem cells are able to differentiate into cardiomyocytes in vitro, the efficiency of this process is very low. Other methods to differentiate progenitor cells into beating cardiomyocytes rely on coculturing with rat ... More