Phalloidin Labeling Probes
Phalloidin Labeling Probes
Citations et références (14)
Invitrogen™

Phalloidin Labeling Probes

Achieve precise and reliable F-actin staining with fluorescent and biotinylated phalloidins. Phalloidin conjugates are widely used in imaging applications to selectively label F-actin in a variety of sample types including fixed and permeabilized cells, tissue sections, and cell-free experiments.
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RéférenceCouleurGamme de longueur d’onde d’excitationType de colorant
A22282Jaune531⁄554Alexa Fluor™ 532
A22281Bleu346⁄442Alexa Fluor™ 350
A30104Violet405/450Alexa Fluor™ Plus 405
A12379Vert495⁄518Alexa Fluor™ 488
O7466Vert496⁄520Oregon Green™ 488
F432Vert496⁄516FITC (fluorescéine)
R415Rouge-orange540⁄565TRITC (isothiocyanate de tétraméthylrhodamine)
A22283Orange556⁄570Alexa Fluor™ 546
A34055Orange555⁄565Alexa Fluor™ 555
A30106Orange555/565 nmAlexa Fluor Plus 555
B3475Rouge558⁄569BODIPY™
A12380Rouge orangé578⁄600Alexa Fluor™ 568
A12381Rouge581⁄609Alexa Fluor™ 594
T7471Rouge591⁄608Texas Red™
A22284Rouge foncé632⁄647Alexa Fluor™ 633
A34054Rouge foncé633⁄647Alexa Fluor™ 635
A22287Rouge foncé650⁄668Alexa Fluor™ 647
A30107Rouge foncé650/668 nmAlexa Fluor Plus 647
A22285Proche infrarouge663⁄690Alexa Fluor™ 660
A22286Proche infrarouge679⁄702Alexa Fluor™ 680
A30105Proche infrarouge758/784Alexa Fluor™ Plus 750
B7474AucunAucunBiotine-XX
P3457AucunAucunPalloïdine (non étiquetée)
Référence A22282
Prix (EUR)
936,65
Online Exclusive
998,00
Économisez 61,35 (6%)
Each
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Couleur:
Jaune
Gamme de longueur d’onde d’excitation:
531⁄554
Type de colorant:
Alexa Fluor™ 532
Prix (EUR)
936,65
Online Exclusive
998,00
Économisez 61,35 (6%)
Each
Ajouter au panier
Fluorescent and biotinylated phalloidins are water soluble and bind to filamentous actin (F-actin) with nanomolar affinity, making them convenient probes for labeling, identifying, and quantifying F-actin in cryopreserved tissue sections, fixed and permeabilized cells, and cell-free experiments. Phalloidin conjugates bind similarly to actin from various species, including plants and animals, enabling staining of the cytoskeleton in a wide range of samples.

A variety of phalloidin conjugates for filamentous (F-actin) staining are available, including fluorescent Alexa Fluor and Alexa Fluor Plus phalloidins, along with phalloidins conjugated to classic fluorescent dyes such as BODIPY, fluorescein, and rhodamine. Phalloidin staining is spectrally compatible with other fluorescent stains used in cellular analyses such as GFP/RFP, Qdot nanocrystals, and other Alexa Fluor conjugates and antibodies. Biotin‐XX Phalloidin can be used to visualize actin filaments via fluorescent streptavidin tags or standard enzyme-mediated avidin/streptavidin techniques such as in electron microscopy. Unlabeled phalloidin is available for use as a control in blocking F‐actin staining or in promoting polymerization.

Phalloidin conjugates bind to both large and small actin filaments with similar affinity in a 1:1 stoichiometry between phallotoxin and actin subunits. They do not bind G-actin monomers.

Alexa Fluor and Alexa Fluor Plus phalloidin conjugates for F-actin staining

Fluorescent Alexa Fluor dye conjugates of phalloidin are popular F-actin stains, offering color choices across the full spectral range. These phalloidin conjugates provide researchers with fluorescent probes that are superior in brightness and photostability compared to other spectrally similar conjugates.

Alexa Fluor Plus Phalloidin conjugates retain the same specificity for actin but offer 3-5 times greater sensitivity and brightness compared to the corresponding Alexa Fluor Phalloidin conjugate. This increased brightness is beneficial for challenging F-actin imaging, such as the super‐resolution microscopy methods SIM and STORM, and for reliable staining of actin stress fibers.

Features of phalloidin probes

  • High specificity—binds selectively to F-actin, which allows for precise labeling of actin filaments in fixed cells and cryopreserved tissues
  • Strong affinity—nanomolar binding affinity for F-actin, which ensures stable and reliable actin staining
  • Extensive fluorescent conjugate options—over twenty conjugated varieties of phalloidin
  • Compatibility with fixed samples—typically used with fixed cells and tissues, making them suitable for actin staining in detailed structural studies, immunofluorescence staining, and IHC applications
  • Multiplexing capability—the wide availability of phalloidin conjugates enables their use in combination with other fluorescent probes and antibodies for multiplex imaging. Biotinylated phalloidin can be made use of in downstream streptavidin steps.
  • Quantitative analysis—can be used for quantitative analysis of F-actin distribution and density within cells, aiding in the study of cytoskeletal dynamics. The unlabeled phalloidin can be titrated as a control.
  • Ease of use—staining is straightforward and quick
  • Excellent stability—exhibit good photostability, which is essential for prolonged imaging sessions and time-lapse studies
  • Wide applicability—used for a range of applications, including studying cell morphology, motility, and the effects of drugs on the actin cytoskeleton

Usage exclusivement réservé à la recherche. Ne pas utiliser pour des procédures de diagnostic.

Spécifications
CouleurJaune
Type de colorantAlexa Fluor™ 532
Gamme de longueur d’onde d’excitation531⁄554
À utiliser avec (équipement)Fluorescence Microscope, Flow Cytometer, Confocal Microscope, Compatible with TRITC/RFP filter set
Gamme de produitsAlexa Fluor
Quantité300 Units
Conditions d’expéditionTempérature ambiante
Type d’étiquetteColorants Alexa Fluor
Type de produitPhalloïdine
SubCellular LocalizationActine, cytosquelette, Cytoskeleton
Unit SizeEach
Contenu et stockage
Stocker au congélateur (entre -5°C et -30°C) à l’abri de la lumière.

Foire aux questions (FAQ)

Can I combine Click-iT or Click-iT Plus reactions with phalloidin conjugates used for actin staining?

We do not recommend using phalloidin conjugates for staining actin in combination with traditional Click-iT or Click-iT Plus reactions since phalloidin is extremely sensitive to the presence of copper.

For staining actin in combination with traditional Click-iT or Click-iT Plus reactions, we recommend using anti-α-actin antibodies for staining actin in the cytoskeleton. You can find a list of our actin antibodies here.

Another option would be to use the Click-iT Plus Alexa Fluor Picolyl Azide Toolkit (Cat. Nos. C10641, C10642, C10643). These Click-iT Plus toolkits provide Copper and Copper protectant separately which makes it easier to titrate the copper concentration to obtain optimal labeling with minimal copper-mediated damage. You may need to optimize the click reaction with the lowest possible concentration of copper and then perform the phalloidin staining.

Find additional tips, troubleshooting help, and resources within our Cell Analysis Support Center.

I'm trying to label my paraffin sections for F-actin with a phalloidin conjugate, but I'm not seeing any signal. Why?

When cells and tissues are treated with solvents such as xylene or acetone (for example during deparaffinization of tissue sections), it affects the F-actin in a way that prevents phalloidins from binding. Phalloidin may be used with cryosections, which are not typically washed with organic solvents, or anti-actin antibodies may be used.

Find additional tips, troubleshooting help, and resources within our Cell Analysis Support Center.

Citations et références (14)

Citations et références
Abstract
Philinopside E, a new sulfated saponin from sea cucumber, blocks the interaction between kinase insert domain-containing receptor (KDR) and alphavbeta3 integrin via binding to the extracellular domain of KDR.
Authors:Tian F, Zhu CH, Zhang XW, Xie X, Xin XL, Yi YH, Lin LP, Geng MY, Ding J,
Journal:Mol Pharmacol
PubMed ID:17565003
'Vascular endothelial growth factor (VEGF) signaling pathway is essential for tumor angiogenesis and has long been recognized as a promising target for cancer therapy. Current view holds that physical interaction between alpha(v)beta(3) integrin and kinase insert domain-containing receptor (KDR) is important in regulating angiogenesis and tumor development. We have reported ... More
Increase in phagocytosis after geldanamycin treatment or heat shock: role of heat shock proteins.
Authors:Vega VL, De Maio A
Journal:J Immunol
PubMed ID:16210633
'The response to injury is activated at the systemic and cellular levels. At the systemic level, phagocytosis plays a key role in controlling infections and clearing necrotic and apoptotic cells. The expression of heat shock proteins (Hsp), which is a well-conserved process, is a major component of cellular response to ... More
A novel mechanism for the formation of actin-filament bundles by a nonprocessive formin.
Authors:Michelot A, Derivery E, Paterski-Boujemaa R, Guérin C, Huang S, Parcy F, Staiger CJ, Blanchoin L,
Journal:Curr Biol
PubMed ID:17027489
Actin-filament bundles (or cables) have a structural role during cell division and morphogenesis, but also serve as important
Two distinct distributions of F-actin are present in the hyphal apex of the oomycete Achlya bisexualis.
Authors:Yu YP, Jackson SL, Garrill A
Journal:Plant Cell Physiol
PubMed ID:15047875
We show that two distinct distributions of F-actin are present in the hyphal apex of the oomycete Achlya bisexualis, that have been chemically fixed with a combination of methylglyoxal and formaldehyde and stained with Alexa phalloidin. In approximately one half of the hyphae examined, an F-actin depleted zone within the ... More
Controlling the behaviour of Drosophila melanogaster via smartphone optogenetics.
Authors:
Journal:Sci Rep
PubMed ID:33077824
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