Citations et références (4)

Invitrogen™

BacLight™ Green Bacterial Stain

Les colorations bactériennes BacLight Green (réf. B-35000) et BacLight Red (réf. B-35001) sont des réactifs de marquage fluorescents pour laAfficher plus

Référence	Quantité
B35000	20 x 50 μg

Référence B35000

Prix (EUR)

451,65

Online Exclusive

602,00

Économisez 150,35 (25%)

Quantité:

20 x 50 μg

Prix (EUR)

451,65

Online Exclusive

602,00

Économisez 150,35 (25%)

Les colorations bactériennes BacLight Green (réf. B-35000) et BacLight Red (réf. B-35001) sont des réactifs de marquage fluorescents pour la détection et la surveillance des bactéries. Ces deux colorants ne sont pas des colorants acides nucléiques. Les bactéries colorées avec les colorations bactériennes BacLight Green et BacLight Red présentent une fluorescence rouge et vert vif (absorption / émission de respectivement environ 480 / 516 et environ 581 / 644 nm), et peuvent être résolues à l’aide des canaux cytométriques à flux approprié ou des filtres de microscopie à fluorescence. Les colorations bactériennes BacLight sont compatibles avec les méthodes de fixation du formaldéhyde ou de l’alcool.

Consultez des informations supplémentaires sur tous les produits d’analyse microbiologique.

Usage exclusivement réservé à la recherche. Ne pas utiliser pour des procédures de diagnostic.

Spécifications

Type de celluleBactéries

Méthode de détectionFluorescent, Fluorescence

Type de colorantAutre(s) étiquette(s) ou colorant(s)

FormeSolide, Solide

FormatTube(s), lame(s), Tube(s), lame(s)

Quantité20 x 50 μg

Conditions d’expéditionTempérature ambiante, Température ambiante

SolubilitéDMSO (diméthylsulfoxyde), DMSO (diméthylsulfoxyde)

Emission480⁄ 561

À utiliser avec (équipement)Microscope à fluorescence, Cytomètre en flux

Gamme de produitsBacLight

Type de produitColoration bactérienne

Unit SizeEach

Contenu et stockage

Contient 20 flacons de coloration bactérienne BacLight™ Green (50 ug par flacon).

Conserver au congélateur (-5 à -30°C) à l’abri de la lumière.

Foire aux questions (FAQ)

What bacterial parameters can I look at by flow cytometry?

You can stain bacteria with a general stain such as BacLight Green Bacterial Stain (Cat. No. B35000) or BacLight Red Bacterial Stain (Cat. No. B35001). You can look at gram character (Cat. No. L7005), cell viability (Cat. Nos. L7007, L7012, and L13152), cell count (Cat. Nos. L34856 and B7277), and cell vitality. Cell vitality can be measured by membrane potential (Cat. No. B34950) or by metabolism (Cat. Nos. B34954 and B34956).

Find additional tips, troubleshooting help, and resources within our Cell Analysis Support Center.

What optical filter do your recommend with the BacLight Green Bacterial Stain?

We recommend filters suitable for fluorescein/FITC with the BacLight Green kit.

Find additional tips, troubleshooting help, and resources within our Cell Analysis Support Center.

Citations et références (4)

Citations et références

Abstract

Identification of broadly protective human antibodies to Pseudomonas aeruginosa exopolysaccharide Psl by phenotypic screening.

Authors:DiGiandomenico A, Warrener P, Hamilton M, Guillard S, Ravn P, Minter R, Camara MM, Venkatraman V, Macgill RS, Lin J, Wang Q, Keller AE, Bonnell JC, Tomich M, Jermutus L, McCarthy MP, Melnick DA, Suzich JA, Stover CK,

Journal:J Exp Med

PubMed ID:22734046

Pseudomonas aeruginosa is a leading cause of hospital-associated infections in the seriously ill, and the primary agent of chronic lung infections in cystic fibrosis patients. A major obstacle to effective control of P. aeruginosa infections is its intrinsic resistance to most antibiotic classes, which results from chromosomally encoded drug-efflux systems ... More

Analysis of adherence, biofilm formation and cytotoxicity suggests a greater virulence potential of Gardnerella vaginalis relative to other bacterial-vaginosis-associated anaerobes.

Authors:Patterson JL, Stull-Lane A, Girerd PH, Jefferson KK,

Journal:Microbiology

PubMed ID:19910411

'Worldwide, bacterial vaginosis (BV) is the most common vaginal disorder in women of childbearing age. BV is characterized by a dramatic shift in the vaginal microflora, involving a relative decrease in lactobacilli, and a proliferation of anaerobes. In most cases of BV, the predominant bacterial species found is Gardnerella vaginalis. ... More

Teleost skin, an ancient mucosal surface that elicits gut-like immune responses.

Authors:Xu Z, Parra D, Gómez D, Salinas I, Zhang YA, von Gersdorff Jørgensen L, Heinecke RD, Buchmann K, LaPatra S, Sunyer JO,

Journal:

PubMed ID:23884653

'Skin homeostasis is critical to preserve animal integrity. Although the skin of most vertebrates is known to contain a skin-associated lymphoid tissue (SALT), very little is known about skin B-cell responses as well as their evolutionary origins. Teleost fish represent the most ancient bony vertebrates containing a SALT. Due to ... More

SadA, a trimeric autotransporter from Salmonella enterica serovar Typhimurium, can promote biofilm formation and provides limited protection against infection.

Authors:Raghunathan D, Wells TJ, Morris FC, Shaw RK, Bobat S, Peters SE, Paterson GK, Jensen KT, Leyton DL, Blair JM, Browning DF, Pravin J, Flores-Langarica A, Hitchcock JR, Moraes CT, Piazza RM, Maskell DJ, Webber MA, May RC, MacLennan CA, Piddock LJ, Cunningham AF, Henderson IR,

Journal:Infect Immun

PubMed ID:21859856

Salmonella enterica is a major cause of morbidity worldwide and mortality in children and immunocompromised individuals in sub-Saharan Africa. Outer membrane proteins of Salmonella are of significance because they are at the interface between the pathogen and the host, they can contribute to adherence, colonization, and virulence, and they are ... More