Microbilles de numération absolue CountBright™, pour cytométrie en flux

Citations et références (7)

Invitrogen™

Microbilles de numération absolue CountBright™, pour cytométrie en flux

Les microbilles de numération absolue CountBright™ constituent une suspension étalonnée de microsphères présentant une fluorescence brillante sur une vaste gammeAfficher plus

Référence	Quantité
C36950	5 ml

Référence C36950

Prix (EUR)

376,65

Online exclusive

448,00

Économisez 71,35 (16%)

Quantité:

Prix (EUR)

376,65

Online exclusive

448,00

Économisez 71,35 (16%)

Les microbilles de numération absolue CountBright™ constituent une suspension étalonnée de microsphères présentant une fluorescence brillante sur une vaste gamme de longueurs d’onde d’excitation et d’émission (UV à 635 nm d’excitation et 385 à 800 nm d’émission). Les microbilles de numération absolue CountBright™ sont mélangées à l’échantillon cellulaire et dosées par la cytométrie en flux. Lorsque vous comparez le rapport des événements de billes aux événements de cellules, vous pouvez calculer le nombre absolu de cellules dans l’échantillon. Étant donné que les microbilles CountBright™ sont mélangées dans l’échantillon pour analyse, le comptage absolu de cellules ayant recours à cette méthode de plateforme unique est plus précis et moins compliqué que la concentration cellulaire déterminée par des essais sur plusieurs plateformes. Les microbilles de comptage absolu CountBright™ peuvent être utilisées avec n’importe quel type d’échantillon, y compris le sang entier sans nettoyage / lyse.

Afficher des informations sur toutes les microbilles de comptage de cellules de cytométrie en flux.

Usage exclusivement réservé à la recherche. Ne pas utiliser pour des procédures de diagnostic.

Spécifications

Méthode de détectionFluorescent

Diamètre (métrique)7 μm

ÉmissionTout laser (UV à 633 / 635)

Gamme de longueur d’onde d’excitationUV de 635 / 385 à 800 nm

À utiliser avec (équipement)Cytomètre en flux

FormatSolution

Nbre de tests100

Quantité5 ml

Conditions d’expéditionTempérature ambiante

Gamme de produitsCountBright

Type de produitMicrobilles de numération absolue

Unit SizeEach

Contenu et stockage

Contient 1 flacon de microbilles de comptage absolu CountBright™ (5 ml). Conserver au réfrigérateur (2°C à 8°C) et à l’abri de la lumière.

Foire aux questions (FAQ)

With the CountBright Plus Absolute Counting Beads/CountBright Absolute Counting Beads, instead of a tight cluster of beads on the scatter plot, I am seeing the beads cover a broad area. What is causing this?

Prior to taking an aliquot, make certain to thoroughly mix the beads. This may also be caused if the beads were ever frozen or the stock solution has any microbial contamination.

Find additional tips, troubleshooting help, and resources within our Cell Analysis Support Center.

Which buffers can I use with the CountBright Plus Absolute Counting Beads and CountBright Absolute Counting Beads?

You may use any physiological buffer or media in a pH range from pH 4 to 10. Avoid extremes of pH and high salt concentrations.

Find additional tips, troubleshooting help, and resources within our Cell Analysis Support Center.

How are CountBright Plus Absolute Counting Beads different from CountBright Absolute Counting Beads?

Here are the differences:

CountBright Plus Absolute Counting Beads:
Bead Size: 4 µm
Excitation Range: 385 - 810 nm
Emission Range: 385 - 860 nm

CountBright Absolute Counting Beads:
Bead Size: 7 µm
Excitation Range: 350 - 635 nm
Emission Range: 350 - 810 nm

Find additional tips, troubleshooting help, and resources within our Cell Analysis Support Center.

When using the Live/Dead BacLight Bacterial Viability and Counting Kit, for flow cytometry, some cells seem to have both red and green signal. Are these cells dead or alive or dying?

The green dye in the kit will label all the cells as it is a cell-permeant nucleic acid stain. The red dye is not cell permeant, and will only stain the cells with compromised membranes (dead cells). Therefore, any cells with red signal will be considered dead. It is possible that you will have some cells that are only red, some that are red and green, and some that are only green. Sometimes the red dye will displace the green dye. In any case, any red cells are dead.

Also, the green dye emission may bleed through into the red channel. Do a single-color staining and examine under both green and red filter sets to determine the level of bleedthrough. To avoid this bleedthrough, use a lower concentration of dye, and, if possible, use narrow bandpass filters.

Find additional tips, troubleshooting help, and resources within our Cell Analysis Support Center.

How can I count my bacteria by flow cytometry?

There are several options. We have two fluorescence based kits that are useful for bacterial counting: Live/Dead BacLight Bacterial Viability and Counting Kit, for flow cytometry (Cat. No. L34856) and Bacteria Counting kit, for flow cytometry (Cat. No. B7277). Another option is the Flow Cytometry Sub-micron Particle Size Reference Kit (Cat. No. F13839).

Find additional tips, troubleshooting help, and resources within our Cell Analysis Support Center.

View all Microbilles de numération absolue CountBright™, pour cytométrie en flux FAQs

Citations et références (7)

Citations et références

Abstract

An impaired transendothelial migration potential of chronic lymphocytic leukemia (CLL) cells can be linked to ephrin-A4 expression.

Authors:Trinidad EM, Ballesteros M, Zuloaga J, Zapata A, Alonso-Colmenar LM,

Journal:Blood

PubMed ID:19828693

'Chronic lymphocytic leukemia (CLL) cell migration into lymphoid tissues is an important aspect of the pathobiology of this disease. Here, we investigated the role of ephrin-A4 (EFNA4) in the transendothelial migration (TEM) capacity of CLL and normal B cells through interacting with endothelial EphA2 (erythropoietin-producing hepatocellular carcinoma). CLL cells showed ... More

Analysis of microRNA and protein transfer by exosomes during an immune synapse.

Authors:Villarroya-Beltri C, Gutiérrez-Vázquez C, Sánchez-Madrid F, Mittelbrunn M,

Journal:

PubMed ID:23719941

'Immune cells release microRNA-containing exosomes that can be taken up by recipient cells. Exosomes can thus act as mediators of cell-cell communication through direct exchange of genetic material between cells. Exosome-mediated transfer of miRNAs between T cells and antigen-presenting cells (APCs) can take place over long distances. Our work has ... More

Inhibition of NF-?B-mediated signaling by the cyclin-dependent kinase inhibitor CR8 overcomes prosurvival stimuli to induce apoptosis in chronic lymphocytic leukemia cells.

Authors:Cosimo E, McCaig AM, Carter-Brzezinski LJ, Wheadon H, Leach MT, Le Ster K, Berthou C, Durieu E, Oumata N, Galons H, Meijer L, Michie AM,

Journal:Clin Cancer Res

PubMed ID:23532892

Chronic lymphocytic leukemia (CLL) is currently incurable with standard chemotherapeutic agents, highlighting the need for novel therapies. Overcoming proliferative and cytoprotective signals generated within the microenvironment of lymphoid organs is essential for limiting CLL progression and ultimately developing a cure. We assessed the potency of cyclin-dependent kinase (CDK) inhibitor CR8, ... More

PKCa negatively regulates in vitro proplatelet formation and in vivo platelet production in mice.

Authors:Williams CM, Harper MT, Poole AW,

Journal:Platelets

PubMed ID:23402219

Proplatelet formation is a part of the intricate process by which platelets are generated by their precursor cell, the megakaryocyte. The processes that drive megakaryocyte maturation and platelet production are however still not well understood. The protein kinase C (PKC) family of serine/threonine kinases has been demonstrated as an important ... More

CD26 inhibition enhances perfusion recovery in ApoE-/-mice.

Authors:Haverslag RT, de Groot D, Grundmann S, Meder B, Goumans MJ, Pasterkamp G, Hoefer IE, de Kleijn DP,

Journal:Curr Vasc Pharmacol

PubMed ID:23391419

The adaptive growth of blood vessels is important to prevent tissue loss following arterial occlusion. Extravasation of monocytes is essential for this process. The peptidase CD26 targets SDF-1 alpha, a chemokine regulating monocyte trafficking. We hypothesized that blocking SDF-1 alpha inactivation, using a commercially available CD26 inhibitor, accelerates perfusion recovery ... More

View all Microbilles de numération absolue CountBright™, pour cytométrie en flux citations