ELISA Buffer Kit

Le kit de tampons ELISA contient tous les tampons de base nécessaires à l’utilisation des paires d’anticorps Invitrogen ou ceuxAfficher plus

Référence	Quantité
CNB0011	10 plaques

Référence CNB0011

Prix (EUR)

320,00

Ajouter au panier

Quantité:

10 plaques

Prix (EUR)

320,00

Ajouter au panier

Le kit de tampons ELISA contient tous les tampons de base nécessaires à l’utilisation des paires d’anticorps Invitrogen ou ceux nécessaires à l’exécution d’un test ELISA. Le kit contient suffisamment de matériau pour 10 plaques ELISA.

Composants du kit :
• Tampon de revêtement A (tampon phosphate 10 mM, pH 7,4)
• Tampon de revêtement B (tampon carbonate 50 mM, pH 9,4)
• Tampon de dosage (tampon de blocage) (5X)
• Tampon de lavage (25X)
• TMB stabilisé
• Solution d’arrêt

Voir tous les tampons et réactifs disponibles pour les applications ELISA.

Usage exclusivement réservé à la recherche. Ne pas utiliser pour des procédures de diagnostic.

Spécifications

À utiliser avec (application)ELISA

FormeConcentrat

Conditions d’expéditionGlace humide

Quantité10 plaques

Unit SizeEach

Contenu et stockage

Tampon de revêtement A (100 ml) : 1 flacon
Tampon de revêtement B (100 ml) : 1 flacon
Tampon de dosage (5x) (200 ml) : 1 flacon
Tampon de lavage (25x) (100 ml) : 3 flacons
Chromogène stabilisé (25 ml) : 4 flacons
Solution d’arrêt (100 ml) : 1 flacon. À stocker entre 2° et 8°C.

Foire aux questions (FAQ)

How do I develop a sandwich ELISA using Cytosets?

Each CytoSets contains capture (coating) antibody, biotinlyated detection antibody, standard and Streptavidin-HRP. Other reagents required are listed in the CytoSets information sheet included with the kit and can be purshased from us separately (Antibody Pair Buffer kit CNB0011, 5x Assay Buffer DS98200, etc.). The information sheet also provides a specific procedure and illustrates an example standard curve which can be obtained when the specific procedure is followed. A general procedure is summarized here:

1) Coat the microplate with diluted capture (coating) antibody overnight at 2-8 degrees C; Wash the plate
2) Incubate standards or samples with the coated microplate; Wash the plate
3) Incubate diluted biotinlyated detection antibody with the plate; Wash the plate
4) Incubate Streptavidin-HRP with the plate for 15-45 minutes; Wash the plate
5) Incubate the plate with TMB substrate for 10-60 minutes and stop the reaction with Stop solution
6) Read microplate at 450 nm.

Investigators are advised to determine optimal buffer formulations, concentrations and incubation times for individual applications.

Find additional tips, troubleshooting help, and resources within our Antibodies and Immunoassays Support Center.

Citations et références (2)

Citations et références

Abstract

Expression of innate immune response genes in liver and three types of adipose tissue in cloned pigs.

Authors:RÃ¸dgaard T, Skovgaard K, Stagsted J, Heegaard PM,

Journal:Cell Reprogram

PubMed ID:22928970

The pig has been proposed as a relevant model for human obesity-induced inflammation, and cloning may improve the applicability of this model. We tested the assumptions that cloning would reduce interindividual variation in gene expression of innate immune factors and that their expression would remain unaffected by the cloning process. ... More

Mechanisms of toxicity induced by SiO2 nanoparticles of in vitro human alveolar barrier: effects on cytokine production, oxidative stress induction, surfactant proteins A mRNA expression and nanoparticles uptake.

Authors:Farcal LR, Uboldi C, Mehn D, Giudetti G, Nativo P, Ponti J, Gilliland D, Rossi F, Bal-Price A,

Journal:Nanotoxicology

PubMed ID:22769972

'Abstract An in vitro human alveolar barrier established by the coculture of epithelial human cell line NCI-H441 with endothelial human cell line ISO-HAS1 was used to evaluate the effects of amorphous silicon dioxide nanoparticles (SiNPs), in the presence or absence of THP-1 cells (monocytes). SiNPs exposure induced production of proinflammatory ... More