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The original iBlot Transfer Stack SKUs will be discontinued effective July 1, 2026 or until inventory is depleted. The latest generation iBlot 3 Dry Blotting System is the recommended replacement and enables twice the throughput and features optional built-in cooling for better consistency. Learn more about the benefits of the iBlot 3 here.
Pile de transfert iBlot™, nitrate de cellulose, mini
Citations et références (1)
Invitrogen™

Pile de transfert iBlot™, nitrate de cellulose, mini

Les empilements de transfert iBlot™ permettent de transférer les protéines à l’aide du dispositif de transfert de gel iBlot™. LesAfficher plus
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RéférenceQuantitéDimensions (LxW)
IB3010323 x 10 ensemblesMini, 8 cm x 8 cm
IB30100210 kitsMini, 8 cm x 8 cm
Référence IB301032
Prix (EUR)
522,00
Each
Quantité:
3 x 10 ensembles
Dimensions (LxW):
Mini, 8 cm x 8 cm
Prix (EUR)
522,00
Each
Les empilements de transfert iBlot™ permettent de transférer les protéines à l’aide du dispositif de transfert de gel iBlot™. Les empilements de transfert iBlot™ sont des empilements de cellules jetables disponibles en trois variantes pour différentes applications : iBlot™ Gel dispose d’une membrane de transfert intégrée en PVDF ou en nitrocellulose pour le transfert à sec de protéines, iBlot™ DNA possède une membrane de transfert intégrée en nylon pour le transfert à sec de l’ADN. Les empilements de détection Western iBlot™ sont utilisés pour effectuer des détections Western sur des membranes en nitrocellulose ou en PVDF préalablement transférées. Chaque empilement de transfert d’iBlot™ contient une électrode en cuivre et des tampons de cathode et d’anode appropriés dans la matrice de gel pour permettre un transfert rapide et fiable de protéines ou d’ADN.

Membrane en nitrate de cellulose
La membrane en nitrate de cellulose (porosité de 0,2 µm) est composée de nitrate de cellulose pur à 100 % pour un transfert de qualité parfaite. La membrane est compatible avec les méthodes de détection courantes telles que la coloration, l’immunodétection, la fluorescence ou le marquage radioactif. Les protéines se lient à la membrane par des interactions hydrophobes et électrostatiques. La capacité de liaison des protéines est de 209 µg/cm2.

Tailles de l’empilement de transfert
La taille standard (13 cm x 8,3 cm) est utilisée pour tous les types de gel midi Novex™ (ou équivalent) et les gels E-PAGE™ 48 ou 96. La taille mini (8 cm x 8 cm) est utilisée pour tous les gels mini Novex™ (ou équivalents).
Usage exclusivement réservé à la recherche. Ne pas utiliser pour des procédures de diagnostic.
Spécifications
Quantité3 x 10 ensembles
Dimensions (LxW)Mini, 8 cm x 8 cm
FormatPile de transfert
Longueur (métrique)8 cm
MatériauNitrocellulose
Porosité0,2 μm
Gamme de produitsiBlot
Suffisant pour30 transferts
Largeur (métrique)8 cm
Unit SizeEach
Contenu et stockage
30 kits d’empilements de transfert en 3 boîtes. Conserver à température ambiante.

Foire aux questions (FAQ)

Following the release of the iBlot 2 Gel Transfer Device, are consumables for the original iBlot Gel Transfer Device still available?

Yes, all original iBlot stacks are still available for purchase. You can find them in the original iBlot Gel Transfer Device Reagents and Resources (http://www.thermofisher.com/us/en/home/life-science/protein-biology/protein-assays-analysis/western-blotting/transfer-proteins-western-blot/iblot-dry-blotting-system/reagents-resources-original-iblot-gel-transfer-device.html). However, please note that they are not compatible with the iBlot 2 Gel transfer Device.

Find additional tips, troubleshooting help, and resources within our Protein Electrophoresis and Western Blotting Support Center.

Is the iBlot 2 Gel Transfer Device compatible with original iBlot Transfer Stacks?

No. Do not use iBlot Transfer Stacks in the iBlot 2 Gel Transfer Device, and do not mix components between iBlot Transfer Stacks and iBlot 2 Transfer Stacks.

Find additional tips, troubleshooting help, and resources within our Protein Electrophoresis and Western Blotting Support Center.

How can I get better transfer of high-molecular weight proteins?

Proteins larger than ~150 kDa migrate more slowly than smaller proteins. Therefore, more time is required to transfer them from gel to membrane. We recommend extending the transfer time by 8 to 10 minutes for optimal transfer of proteins >150 kDa using the iBlot Dry Blotting System.

To enhance transfer efficiency, we also recommend adding an equilibration (gel-soaking) step between electrophoresis and western transfer and using NuPAGE Invitrogen 3 - 8% Tris-acetate gels for electrophoresis. We have an application note available, titled “Transferring Large and Small Proteins Using the iBlot Dry Blotting System”. To download the PDF, see the iBlot Reagents Resources page. (http://www.thermofisher.com/us/en/home/life-science/protein-expression-and-analysis/western-blotting/western-blot-transfer/iblot-dry-blotting-system/original-iblot-resources.html)

Find additional tips, troubleshooting help, and resources within our Protein Electrophoresis and Western Blotting Support Center.

Occasionally my western blots have high background. What do you recommend?

This may be a result of insufficient blocking or nonspecific binding. We suggest trying our WesternBreeze Blocker/Diluent (Cat. No. WB7050). We have been using it with good success. Additionally, you should optimize primary and secondary antibody concentrations as generally recommended for any new blotting technique. Many cases of high background can be resolved by further diluting one or both antibody preparations.

Find additional tips, troubleshooting help, and resources within our Protein Electrophoresis and Western Blotting Support Center.

Is it possible to substitute the membrane from the iBlot Transfer Stack with my specialized membrane?

In theory, you can replace the membrane provided in your iBlot Transfer Stack with any membrane that is compatible with western blotting. To do this, cut the alternative membrane to match the size of your gel, and wet the membrane. Then, either place the alternative membrane on top of the integrated membrane, or carefully remove the integrated membrane from the gel matrix with forceps and replace it with the new membrane. Note that we only support the use of iBlot Transfer Stacks when they are used with the provided instructions.

Find additional tips, troubleshooting help, and resources within our Protein Electrophoresis and Western Blotting Support Center.

View all Pile de transfert iBlot™, nitrate de cellulose, mini FAQs

Citations et références (1)

Citations et références
Abstract
Transcriptome-wide identification of RNA binding sites by CLIP-seq.
Authors:Murigneux V, Saulière J, Roest Crollius H, Le Hir H,
Journal:Methods
PubMed ID:23545196
An emergent strategy for the transcriptome-wide study of protein-RNA interactions is CLIP-seq (crosslinking and immunoprecipitation followed by high-throughput sequencing). We combined CLIP-seq and mRNA-seq to identify direct RNA binding sites of eIF4AIII in human cells. This RNA helicase is a core constituant of the Exon Junction Complex (EJC), a multifunctional ... More