NovaFluor™ Antibody Conjugation Kits

Invitrogen™

NovaFluor™ Antibody Conjugation Kits

Name: NovaFluor™ Antibody Conjugation Kits
SKU: K06T04L012
Price: 488.00 EUR

Tirez le meilleur parti de votre conception de panneaux en utilisant des anticorps conjugués aux NovaFluors : colorants spécifiquement conçus pour l’hygiène spectrale afin d’avoir des spectres d’émission étroits et une excitation laser croisée minimale.

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Référence	Marqueur ou colorant	Type de produit	Émission	Quantité
K06T04L012	NovaFluor™ Bleu 660/120S	Kit de conjugaison	660 nm	100 μg
K06T04L008	NovaFluor™ Bleu 510	Kit de conjugaison	510 nm	100 μg
K06T04L002	NovaFluor™ Bleu 530	Kit de conjugaison	530 nm	100 μg
K06T04L009	NovaFluor™ Bleu 555	Kit de conjugaison	555 nm	100 μg
K06T04L010	NovaFluor™ Bleu 585	Kit de conjugaison	585 nm	100 μg
K06T04L003	NovaFluor™ Bleu 610/30S	Kit de conjugaison	610 nm	100 μg
K06T04L011	NovaFluor™ Bleu 610/70S	Kit de conjugaison	610 nm	100 μg
K06T04L004	NovaFluor™ Bleu 660/40S	Kit de conjugaison	660 nm	100 μg
K06T04L021	NovaFluor™ Bleu 690	Kit de conjugaison	690 nm	100 μg
K06T04L026	NovaFluor™ Bleu 760	Kit de conjugaison	760 nm	100 μg
K06T04L017	NovaFluor™ Rouge 660	Kit de conjugaison	660 nm	100 μg
K06T04L018	NovaFluor™ Rouge 685	Kit de conjugaison	685 nm	100 μg
K06T04L019	NovaFluor™ Rouge 700	Kit de conjugaison	700 nm	100 μg
K06T04L020	NovaFluor™ Rouge 710	Kit de conjugaison	710 nm	100 μg
K06T04L023	NovaFluor™ Rouge 725	Kit de conjugaison	725 nm	100 μg
K06T04L024	NovaFluor™ Rouge 755	Kit de conjugaison	755 nm	100 μg
K06T04L005	NovaFluor™ Jaune 570	Kit de conjugaison	570 nm	100 μg
K06T04L013	NovaFluor™ Jaune 590	Kit de conjugaison	590 nm	100 μg
K06T04L006	NovaFluor™ Jaune 610	Kit de conjugaison	610 nm	100 μg
K06T04L007	NovaFluor™ Jaune 660	Kit de conjugaison	660 nm	100 μg
K06T04L014	NovaFluor™ Jaune 690	Kit de conjugaison	690 nm	100 μg
K06T04L015	NovaFluor™ Jaune 700	Kit de conjugaison	700 nm	100 μg
K06T04L016	NovaFluor™ Jaune 730	Kit de conjugaison	730 nm	100 μg
K06T04L022	NovaFluor™ Jaune 755	Kit de conjugaison	755 nm	100 μg
K06T04L027	NovaFluor™ UV 765	Kit de conjugaison	765 nm	100 μg
K06T04L028	NovaFluor™ Violet 800	Kit de conjugaison	800 nm	100 μg
K06T04L029	NovaFluor™ Violet 745	Kit de conjugaison	745 nm	100 μg
K06T04L030	NovaFluor™ Jaune 810	Kit de conjugaison	810 nm	100 μg
K06T04L031	NovaFluor™ Bleu 725	Kit de conjugaison	725 nm	100 μg
K06T04L032	NovaFluor™ Violet 690	Kit de conjugaison	690 nm	100 μg
K06T04L033	NovaFluor™ Bleu 800	Kit de conjugaison	800 nm	100 μg
K06T04L034	NovaFluor™ Blue 745	Conjugation Kit	748	100 μg
K06T04L035	NovaFluor™ Red 800	Conjugation Kit	799	100 μg

33 options

Référence K06T04L012

Prix (EUR)

488,00

Each

Ajouter au panier

Marqueur ou colorant:

NovaFluor™ Bleu 660/120S

Type de produit:

Kit de conjugaison

Émission:

660 nm

Quantité:

100 μg

Prix (EUR)

488,00

Each

Ajouter au panier

Les kits de conjugaison NovaFluor permettent de conjuguer un fluorophore excitable bleu, jaune-vert ou rouge à l’anticorps de votre choix en seulement 3 étapes. La plateforme de fluorescence Phiton permet l’ajout de paramètres à vos panneaux actuels de recherche sur la découverte et résout les populations colorées avec une excitation croisée proche de zéro, avec un manque d’émission dans les canaux violets qui perturbent les autres fluorophores.

NovaFluor Conjugation Kit provides features including:

A clean and bright antibody label to increase the number of parameters on conventional and spectral flow cytometers
Resolve populations with limited/near-zero cross excitation and the lowest spread performance of any blue, yellow-green or red excited fluorescent labels
Simple 3-step protocol, with 60 sec. of hands-on time for conjugating 10–100 μg of antibody per label
Up to 70% less excitation when compared to PE tandems, resulting in decreased spillover and higher resolution

3-step conjugation method

Attach NovaFluor Linker to antibody of choice
Remove excess NovaFluor Linker from DNA-conjugate antibody
Anneal NovaFluor to oligo-conjugated antibody

Usage exclusivement réservé à la recherche. Ne pas utiliser pour des procédures de diagnostic.

Spécifications

Émission660 nm

À utiliser avec (équipement)Cytométrie en flux

Marqueur ou colorantNovaFluor™ Bleu 660/120S

Type de produitKit de conjugaison

Quantité100 μg

Gamme de produitsNovaFluor

Unit SizeEach

Foire aux questions (FAQ)

What is the NovaFluor dye structure and how does this impact the NovaFluor antibody conjugation workflow?

NovaFluor dyes are based on Phiton technology utilizing novel nucleic acid dye structures. The dyes work with FRET technology and have a clear benefit versus tandem dyes, as the structures are extremely stable. More information can be found on our NovaFluor website.

Due to the nucleotide structure, some adherence of the NovaFluor dyes to dead cells is expected. Good viability of the sample and inclusion of a fixable viability stain is recommended. Using nucleotide-staining viability dyes such as DAPI, PI, and 7-AAD dyes is not recommended.

Find additional tips, troubleshooting help, and resources within our Cell Analysis Support Center.

Can I use NovaFluor antibody conjugation kits for conjugation of antibodies targeting intracellular epitopes?

NovaFluor antibody conjugation kits can be used for conjugation of antibodies targeting intracellular epitopes. The labeling needs to be performed with 10 µL CellBlox Plus Blocking Buffer (Cat. No. C001T02F01, C001T03F01, C001T06F01) to block non-specific binding of NovaFluor labels with cells, after fixation and permeabilization.

If NovaFluor dyes are used for surface targets as well as intracellular targets, first add 5 µL CellBlox Plus Blocking Buffer during staining with the surface markers. Then, after completing the staining, washing, fixation, and permeabilization, add 10 µL CellBlox Plus Blocking Buffer again to block non-specific intracellular binding while labeling with the NovaFluor conjugates for intracellular targets.

Find additional tips, troubleshooting help, and resources within our Cell Analysis Support Center.

Can I label my antibody with more than one NovaFluor label if I purchased more than one NovaFluor conjugation kit?

Yes, you can. After performing the steps on Days 1 and 2, your Antibody-NovaFluor Linker conjugate can be attached to the NovaFluor label at whatever volume you like, as long as the ratio of the Antibody-NovaFluor Linker to the NovaFluor label is kept constant.

Example: If you purchased two NovaFluor conjugation kits, one with NovaFluor Blue 610-30S and one with NovaFluor Yellow 610, use the contents of both kits to modify your two antibodies of interest (both 100 µg), completing all steps outlined for Days 1 and 2 with no modification. After resuspending your antibodies into PBS and after the second round of ammonium sulfate precipitation, you should have 100 µL of each Antibody-NovaFluor Linker conjugate. You can now divide the antibody solutions in half (50 µL each) and add half of each NovaFluor label to the divided antibody solutions (150 µL of NovaFluor label to each 50 µL of Antibody-NovaFluor Linker), giving you 200 µL total of each antibody conjugated to both NovaFluor Blue 610-30S and NovaFluor Yellow 610. After mixing, continue with the protocol and incubate overnight at 4 degrees C.

Find additional tips, troubleshooting help, and resources within our Cell Analysis Support Center.

How should I store NovaFluor-antibody conjugates?

We recommend storing new NovaFluor-antibody conjugates at 2-8 degrees C, protected from light. If desired, NovaFluor-antibody conjugates can be stored with 0.09% sodium azide. DO NOT store NovaFluor-antibody conjugates at -80 degrees C or -20 degrees C.

Find additional tips, troubleshooting help, and resources within our Cell Analysis Support Center.

For the NovaFluor conjugation kits, in Step 16 of the procedure, can you provide more information about how I should orient my tube and describe the appearance of the pellet following precipitation?

We have included Figure 4 in the manual (https://assets.thermofisher.com/TFS-Assets/LSG/manuals/MAN0025061_NovaFluorConjugationKit_UG.pdf) to show how the pellet appears following the precipitation step. Although the microcentrifuge tube can be centrifuged in any orientation, we recommend orienting it in the same direction for both centrifugation steps so that your pellet is in the same place (Figure 3). The pellet is often smaller after the second precipitation, so it is helpful to know where to expect it.

Find additional tips, troubleshooting help, and resources within our Cell Analysis Support Center.

View all NovaFluor™ Antibody Conjugation Kits FAQs