TOPO™ TA Cloning™ Kit, Dual Promoter, with One Shot™ TOP10 chemically competent <i>E. coli</i> cells

Citations et références (49)

Invitrogen™

TOPO™ TA Cloning™ Kit, Dual Promoter, with One Shot™ TOP10 chemically competent E. coli cells

Name: TOPO™ TA Cloning™ Kit, Dual Promoter, with One Shot™ TOP10 chemically competent E. coli cells
SKU: K460001
Price: 843.65 EUR

Les kits de double promoteur TOPO™ TA Cloning™ sont conçus pour un clonage rapide et efficace et une transcription inAfficher plus

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Référence	Quantité
K460001	25 réactions
K4600J10	10 réactions
K460040	50 réactions

3 options

Référence K460001

Prix (EUR)

843,65

Online Exclusive

898,00

Économisez 54,35 (6%)

Each

Quantité:

25 réactions

Prix (EUR)

843,65

Online Exclusive

898,00

Économisez 54,35 (6%)

Each

Les kits de double promoteur TOPO™ TA Cloning™ sont conçus pour un clonage rapide et efficace et une transcription in vitro ultérieure. Ces kits incluent le vecteur TA pCR™II-TOPO™ avec deux promoteurs T7 et SP6. En éliminant le clonage fastidieux de sites de restriction, le clonage TOPO™ est la méthode de clonage la plus fiable, qui comprend un protocole en 3 étapes et une réaction de clonage en 5 minutes, et qui produit jusqu’à 95 % de recombinants.

Caractéristiques pratiques de ce kit de double promoteur TOPO™ TA Cloning™ :
• Saillies 3´-T pour la ligature directe de produits PCR amplifiés par Taq
• Promoteurs T7 et SP6 pour une transcription in vitro efficace
• des sites d’amorces sens et antisens M13 pour le séquençage
• 16 sites de restriction pratiques, notamment EcoRI, flanquant votre insert pour une excision ou un sous-clonage ultérieur
• Gènes de résistance à la kanamycine et à l’ampicilline permettant de choisir la méthode de sélection dans E. coli
• sélection de colonies bleues/blanches facile pour la sélection de recombinants
• Comprend des cellules compétentes OneShot™ TOP10 pour plus de commodité et une efficacité de clonage optimale

options de kit : Kits de double promoteur TOPO™ TA Cloning™
Les kits de double promoteur TOPO™ TA Cloning™ peuvent être achetés avec une variété de cellules compétentes qui offrent différents avantages en fonction de vos besoins :
• Clonage général : Cellules TOP10 (No de catalogue K4600-J10, K4600-01, K4600-40)
• Clonage haute efficacité : Cellules TOP10 Electrocomp™ (No de catalogue K4660-01, K4660-40)
• Clonage général, résistance au bactériophage T1 : Cellules DH5α-T1R (No de catalogue K4620-01, K4620-40)
• Croissance rapide : E. Coli chimiquement compétent Mach1™ -T1R (No de catalogue K4610-20)
• Besoins de répresseur/induction : Cellules TOP10F’ (No de catalogue K4650-01, K4650-40)
• Fournissez vos propres cellules (No de catalogue 451641, 450641)

Usage exclusivement réservé à la recherche. Ne pas utiliser pour des procédures de diagnostic.

Spécifications

Souche bactérienne ou de levuresTOP10

Type de celluleChimiquement compétent

Méthode de clonageTOPO™-TA

Gamme de produitsOne Shot

Type de produitKit de clonage

AccélérateurT7, SP6

Quantité25 réactions

VecteurVecteurs de clonage TOPO-TA

FormatKit

Unit SizeEach

Contenu et stockage

Boîte 1 :
• Vecteur pCRII-TOPO™ activé par topoisomérase I
•Tampon PCR
• Solution saline
• dNTPs
• Modèle de contrôle
• Amorces directes et inverses M13
• Amorces pour PCR de contrôle
• Eau stérile

Stockez entre -5 et -30°C.
Tous les réactifs sont stables pendant 6 mois lorsqu’ils sont correctement stockés.

Encadré 2 :
• Cellules d’E. coli chimiquement compétentes One Shot™ ou Electrocomp™
• Milieu S.O.C.
• Plasmide de contrôle pUC19 superenroulé.

Stockez entre -68 et -85°C.

Foire aux questions (FAQ)

Can I store my competent E. coli in liquid nitrogen?

We do not recommend storing competent E. coli strains in liquid nitrogen as the extreme temperature can be harmful to the cells. Also, the plastic storage vials are not intended to withstand the extreme temperature and may crack or break.

How should I store my competent E. coli?

We recommend storing our competent E. coli strains at -80°C. Storage at warmer temperatures, even for a brief period of time, will significantly decrease transformation efficiency.

What is the difference between the pCR2.1-TOPO and pCR4-TOPO vectors?

The vector backbones for both of these vectors are very similar. The main difference is that the pCR4-TOPO vector has sequencing primer sites located as close as 33 base pairs from the PCR product insertion site. This minimizes the amount of vector DNA sequence that needs to be read before reaching the sequence of the insert, making the pCR4-TOPO vector very useful for sequencing applications.

What is the difference between the pCR2.1-TOPO and pCRII-TOPO vectors?

The vector backbones for both of these vectors are very similar. The main difference is that the pCRII-TOPO vector is a dual promoter vector, containing the SP6 and T7 promoters for in vitro transcription/sequencing, whereas the pCR2.1-TOPO vector contains only the T7 promoter for in vitro transcription/sequencing. Both vectors contain the M13 Forward and Reverse primer sites for sequencing or PCR screening.

Your TOPO cloning kits contain a control template and control primers. Can I obtain the sequence of the control template?

The sequence of the control template is proprietary.

View all TOPO™ TA Cloning™ Kit, Dual Promoter, with One Shot™ TOP10 chemically competent E. coli cells, 25 Reactions FAQs

Citations et références (49)

Citations et références

Abstract

Cloning and functional expression of a thyrotropin receptor cDNA from rat fat cells.

Authors:Endo T; Ohta K; Haraguchi K; Onaya T;

Journal:J Biol Chem

PubMed ID:7738021

Thyrotropin receptor (TSH-R) has been thought to be thyroid-specific, but, by Northern blot analysis, we found that rat adipose tissue expressed TSH-R mRNAs in amounts approaching those in the thyroid. To investigate the function of TSH-R from adipose tissue, we screened a rat fat cell lambda gt11 cDNA library for ... More

Modulation of mouse Paneth cell alpha-defensin secretion by mIKCa1, a Ca2+-activated, intermediate conductance potassium channel.

Authors: Ayabe Tokiyoshi; Wulff Heike; Darmoul Dalila; Cahalan Michael D; Chandy K George; Ouellette Andre J;

Journal:J Biol Chem

PubMed ID:11724775

'Paneth cells in small intestinal crypts secrete microbicidal alpha-defensins in response to bacteria and bacterial antigens (Ayabe, T., Satchell, D. P., Wilson, C. L., Parks, W. C., Selsted, M. E., and Ouellette, A. J. (2000) Nat. Immunol. 1, 113- 38). We now report that the Ca(2+)-activated K(+) channel mIKCa1 modulates ... More

Variant forms of alpha-fetoprotein transcripts expressed in human hematopoietic progenitors. Implications for their developmental potential towards endoderm.

Authors: Kubota Hiroshi; Storms Robert W; Reid Lola M;

Journal:J Biol Chem

PubMed ID:12006569

'Hematopoietic stem cells have been identified as multipotent cells that give rise to all adult hematopoietic lineages. Although the hematopoietic lineage is derived from the mesodermal germ layer in the embryo, recent data suggest that bone marrow cells with an antigenic profile consistent with that of hematopoietic stem cells can ... More

Mutation of the matrix metalloproteinase At2-MMP inhibits growth and causes late flowering and early senescence in Arabidopsis.

Authors: Golldack Dortje; Popova Olga V; Dietz Karl-Josef;

Journal:J Biol Chem

PubMed ID:11726650

'This study characterizes the expression and functional significance of the member of the matrix metalloproteinase (MMP) family At2-MMP from Arabidopsis. By transcript analysis, expression of At2-MMP was found in leaves and roots of juvenile Arabidopsis and leaves, roots, and inflorescences of mature flowering plants showing strong increase of transcript abundance ... More

Phosphorylation of the Carboxyl Terminus of Inner Centromere Protein (INCENP) by the Aurora B Kinase Stimulates Aurora B Kinase Activity.

Authors: Bishop John D; Schumacher Jill M;

Journal:J Biol Chem

PubMed ID:12048181

'How the events of mitosis are coordinated is not well understood. Intriguing mitotic regulators include the chromosomal passenger proteins. Loss of either of the passengers inner centromere protein (INCENP) or the Aurora B kinase results in chromosome segregation defects and failures in cytokinesis. Furthermore, INCENP and Aurora B have identical ... More

View all TOPO™ TA Cloning™ Kit, Dual Promoter, with One Shot™ TOP10 chemically competent E. coli cells, 25 Reactions citations