Citations et références (5)

Invitrogen™

SafeStain SimplyBlue™

Name: SafeStain SimplyBlue™
SKU: LC6060
Price: 305.00 EUR

SimplyBlue SafeStain est un colorant Coomassie G-250 simple d’utilisation, rapide, sensible et sans danger pour la visualisation des bandes deAfficher plus

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Référence	Quantité
LC6060	1 l
LC6065	3,5 l

2 options

Référence LC6060

Prix (EUR)

305,00

Each

Quantité:

1 l

Grand volume ou format personnalisé

Prix (EUR)

305,00

Each

SimplyBlue SafeStain est un colorant Coomassie G-250 simple d’utilisation, rapide, sensible et sans danger pour la visualisation des bandes de protéines sur les gels de polyacrylamide et sur les membranes sèches en PVDF. Ce colorant est absolument sans danger et ne nécessite ni décolorations ni fixateurs de type méthanol ou acide acétique. Le risque d’exposition à des produits dangereux et à des odeurs désagréables est éliminé, créant ainsi un environnement de laboratoire plus sain.

Comparer toutes les colorations Coomassie ›

Protocole simple d’utilisation
Le protocole SimplyBlue SafeStain est facile à exécuter et peut être complété en moins de trois heures. Pour une vitesse supérieure, une procédure de micro-ondes simple peut être exécutée en 12 minutes. Aucune décoloration n’est nécessaire, mais celle-ci peut être effectuée avec de l’eau déionisée pour obtenir un degré de sensibilité maximal, notamment lors de l’analyse en aval, comme la spectrométrie de masse ou lorsqu’un fond transparent est requis.

Usage exclusivement réservé à la recherche. Ne pas utiliser pour des procédures de diagnostic.

Spécifications

Emplacement de détectionDétection sur gel

Méthode de détectionColorimétrique

Quantité1 l

Durée de conservation6 mois

Molécule cibleProtéine

CouleurBlue

Marqueur ou colorantCoomassie

Gamme de produitsSimplyBlue

Type de produitColoration fiable des protéines

Unit SizeEach

Contenu et stockage

Le SimplyBlue™ SafeStain est fourni sous forme de réactif de coloration prêt à l’emploi 1X. Conserver à température ambiante. Garantie stable pendant 6 mois sous réserve d’un stockage correct. Aucun frais HazMat n’est associé à ce produit.

Foire aux questions (FAQ)

Can I use the Colloidal Blue Staining Kit (Cat. No. LC6025) for membrane staining to detect proteins that are transferred to a membrane?

It is not recommended because the background will be too high. Better alternatives include: 1) Invitrogen Reversible Membrane Protein Stain Kit (Cat. No. IB7710).

2) Coomassie (non-colloidal) staining: stain in 0.1% Coomassie Blue R-250 in 50% methanol for 5 min and destain with several changes of 50% methanol and 10% acetic acid. Rinse with several changes of water, air dry and store for up to 12 months at -20°C. Sensitivity is approximately at the 50-100 ng level.

3) Use SimplyBlue SafeStain (Cat. No. LC6060). The SimplyBlue SafeStain manual has the protocol for staining PVDF membranes, but it is not recommended for nitrocellulose because of high background.

4) Amido Black: same as Coomassie but less sensitive.

5) Ponceau S: same as Coomassie but less sensitive.

6) UV transillumination: place membrane on filter paper after blot is finished and allow to dry at room temperature for about 10 min. Rewet in 20% methanol and view the blot in front of white light while it is still wet; the bands will look more translucent than the membrane. If the bands disappear as the membranes dries, rewet again.

Find additional tips, troubleshooting help, and resources within our Protein Electrophoresis and Western Blotting Support Center.

Why is Coomassie G-250 used as the tracking dye in NuPAGE LDS Sample Buffer instead of bromophenol blue?

Coomassie G-250 will give a sharp dye front with both NuPAGE MES and MOPS Running Buffers and is therefore used as the tracking dye in the NuPAGE LDS Sample Buffer.

Bromophenol blue runs more slowly than some peptides with the NuPAGE MES Running Buffer system.

Coomassie G-250 migrates much closer to the moving ion front than bromophenol blue, ensuring that small peptides will not be run too far (e.g., off the gel).

Find additional tips, troubleshooting help, and resources within our Protein Electrophoresis and Western Blotting Support Center.

Is the SimplyBlue SafeStain the appropriate stain for quantitation by densitometry?

The Colloidal Blue Staining Kit (Cat. No. LC6025) is best for quantitation by densitometry. You can also use SimplyBlue SafeStain for this application.

The great advantage of SimplyBlue SafeStain is that it is very easy to use and safe.

Find additional tips, troubleshooting help, and resources within our Protein Assays and Analysis Support Center.

The sensitivity of my SimplyBlue SafeStain seems to be decreasing over time. Why is this?

Check the cap on the bottle. If the bottles are not tightly sealed, the alcohol can evaporate from the stain causing substantial decrease in stain sensitivity.

Find additional tips, troubleshooting help, and resources within our Protein Assays and Analysis Support Center.

How do I destain proteins on a PVDF membrane that were stained with SimplyBlue SafeStain?

After staining with SimplyBlue SafeStain, use deionized water for the less strongly retained protein bands on the PVDF membrane.

Increasing methanol or ethanol concentrations up to 70% should destain any remaining bands. You can leave the membrane in the destain indefinitely.

Find additional tips, troubleshooting help, and resources within our Protein Assays and Analysis Support Center.

View all SafeStain SimplyBlue™ FAQs

Citations et références (5)

Citations et références

Abstract

Enzymatic hydrolysis of pyridoxine-5'-beta-D-glucoside is catalyzed by intestinal lactase-phlorizin hydrolase.

Authors: Mackey Amy D; Henderson George N; Gregory Jesse F 3rd;

Journal:J Biol Chem

PubMed ID:12023280

'An obligatory step in the mammalian nutritional utilization of pyridoxine-5''-beta-D-glucoside (PNG) is the intestinal hydrolysis of its beta-glucosidic bond that releases pyridoxine (PN). This laboratory previously reported the purification and partial characterization of a novel cytosolic enzyme, designated PNG hydrolase, which hydrolyzed PNG. An investigation of the subcellular distribution of ... More

2F3 monoclonal antibody recognizes the O26 O-antigen moiety of the lipopolysaccharide of enterohemorrhagic Escherichia coli strain 4276.

Authors:Szalo IM, Taminiau B, Goffaux F, Pirson V, McCappin J, Ball HJ, Mainil JG,

Journal:Clin Diagn Lab Immunol

PubMed ID:15138178

'Enterohemorrhagic Escherichia coli (EHEC) and enteropathogenic E. coli (EPEC) organisms are groups of pathogenic strains whose infections are characterized by a typical lesion of enterocyte attachment and effacement. They are involved in enteric diseases both in humans and in animals, and EHEC strains can be responsible for hemolytic uremic syndrome ... More

Selective fluorescent labeling of S-nitrosothiols (S-FLOS): a novel method for studying S-nitrosation.

Authors:Santhanam L, Gucek M, Brown TR, Mansharamani M, Ryoo S, Lemmon CA, Romer L, Shoukas AA, Berkowitz DE, Cole RN,

Journal:Nitric Oxide

PubMed ID:18706513

'Protein S-nitrosation is a reversible post-translation modification critical for redox-sensitive cell signaling that is typically studied using the Biotin Switch method. This method and subsequent modifications usually require avidin binding or Western blot analysis to detect biotin labeled proteins. We describe here a modification of the Biotin Switch assay that ... More

Vascular endothelial growth factor-C and C-C chemokine receptor 7 in tumor cell-lymphatic cross-talk promote invasive phenotype.

Authors:Issa A, Le TX, Shoushtari AN, Shields JD, Swartz MA,

Journal:Cancer Res

PubMed ID:19118020

'Most carcinomas spread to distant sites through lymphatic vessels. Several preclinical and clinical studies have shown a positive correlation between the incidence of lymph node metastasis and secretion of the lymphatic growth factor vascular endothelial growth factor-C (VEGF-C) by tumor cells, suggesting tumor lymphangiogenesis as an escape mechanism. However, recent ... More

Yeast Expression and NMR Analysis of the Extracellular Domain of Muscle Nicotinic Acetylcholine Receptor alpha Subunit.

Authors: Yao Yun; Wang Junmei; Viroonchatapan Nitnara; Samson Avraham; Chill Jordan; Rothe Elizabeth; Anglister Jacob; Wang Zuo-Zhong;

Journal:J Biol Chem

PubMed ID:11812776

The alpha subunit of the nicotinic acetylcholine receptor (AChR) from Torpedo electric organ and mammalian muscle contains high affinity binding sites for alpha-bungarotoxin and for autoimmune antibodies in sera of patients with myasthenia gravis. To obtain sufficient materials for structural studies of the receptor-ligand complexes, we have expressed part of ... More