NeuroTrace™ 640/660 Deep-Red Fluorescent Nissl Stain - Solution in DMSO

Citations et références (20)

Invitrogen™

NeuroTrace™ 640/660 Deep-Red Fluorescent Nissl Stain - Solution in DMSO

La coloration de Nissl est une méthode histologique standard pour visualiser les neurones dans le cerveau et la moelle épinière.Afficher plus

Référence	Quantité
N21483	1 ml

Référence N21483

Prix (EUR)

438,00

Quantité:

Prix (EUR)

438,00

La coloration de Nissl est une méthode histologique standard pour visualiser les neurones dans le cerveau et la moelle épinière. Composé d’ARN ribosomique associé au réticulum endoplasmique rugueux dans les périkaryons et les dendrites des neurones, la substance de Nissl assure une redistribution au sein du corps cellulaire dans les neurones lésées ou en cours de régénération, tout en fournissant un marqueur de l’état physiologique du neurone. Notre coloration de Nissl NeuroTrace 640/660 fluorescente rouge profond est sélective pour la substance Nissl caractéristique des neurones et offre meilleure sensibilité que les colorants histologiques traditionnels comme le bleu de toluidine ou le violet de crésyle.

Usage exclusivement réservé à la recherche. Ne pas utiliser pour des procédures de diagnostic.

Spécifications

CouleurRouge profond

Méthode de détectionFluorescence

À utiliser avec (équipement)Microscope à fluorescence

Type de produitColoration de Nissl

Quantité1 ml

Conditions d’expéditionTempérature ambiante

Localisation subcellulaireCorps de Nissl

Excitation/Emission640/660 nm

Gamme de produitsNEUROTRACE

Unit SizeEach

Contenu et stockage

Conservez au congélateur (entre -5 et -30°C) à l’abri de la lumière.

Foire aux questions (FAQ)

I am labeling brain cryosections with a NeuroTrace Nissl Stain. Is this compatible with antibody labeling?

Yes. We have done this successfully with an anti-GFAP primary and an Alexa Fluor secondary antibody. We would recommend labeling with the primary and secondary antibodies first, then following up with the standard NeuroTrace Nissl Stain protocol.

Find additional tips, troubleshooting help, and resources within our Cell Analysis Support Center.

I can use the NeuroTrace Nissl stains for staining glia or other cell types. What can I do to improve the staining so that it is more selective for neurons?

Our NeuroTrace Nissl stains label the Nissl substance which is composed of ribosomal RNA associated with the rough endoplasmic reticulum and is present in high amounts in neuronal cells. These dyes are not completely specific for neurons, but will selectively stain neurons based on their high level of protein synthesis. In some cases they may show staining of other cell types such as glia, so you may need to decrease the staining concentration to obtain more selective neuronal labeling. We suggest dilutions in the range of 20- to 300-fold for neuronal staining.

Find additional tips, troubleshooting help, and resources within our Cell Analysis Support Center.

Can the NeuroTrace Nissl stains be used on paraffin sections?

We have only tested them on mouse brain cryosections, however, there is at least one citation describing their use on paraffin tissue sections (Michelle L. Schlief, Ann Marie Craig, and Jonathan D. Gitlin. NMDA Receptor Activation Mediates Copper Homeostasis in Hippocampal Neurons. The Journal of Neuroscience, January 5, 2005, 25(1):239 - 246).

Find additional tips, troubleshooting help, and resources within our Cell Analysis Support Center.

What are the NeuroTrace Nissl stains?

The dyes are proprietary, however they are stains that label the Nissl substance, which is composed of ribosomal RNA associated with the rough endoplasmic reticulum and is present in high amounts in neuronal cells.

Find additional tips, troubleshooting help, and resources within our Cell Analysis Support Center.

What products do you have for neuronal tracing?

Please check out this web page (https://www.thermofisher.com/us/en/home/life-science/cell-analysis/cell-tracing-tracking-and-morphology/neuronal-tracing.html) for details.

Find additional tips, troubleshooting help, and resources within our Cell Analysis Support Center.

Citations et références (20)

Citations et références

Abstract

Glutamate acting on AMPA but not NMDA receptors modulates the migration of hippocampal interneurons.

Authors:Manent JB, Jorquera I, Ben-Ari Y, Aniksztejn L, Represa A,

Journal:J Neurosci

PubMed ID:16738232

'Paracrine GABA and glutamate acting, respectively, on GABAA and NMDA receptors modulate the migration of hippocampal pyramidal cells. Using corticohippocampal organotypic explants from glutamic acid decarboxylase (GAD) 67-enhanced green fluorescent protein (EGFP) knock-in embryos, we now report that, in contrast to pyramidal neurons, the blockade of AMPA but not NMDA ... More

Differential roles of the dopamine 1-class receptors, D1R and D5R, in hippocampal dependent memory.

Authors:Sariñana J, Kitamura T, Künzler P, Sultzman L, Tonegawa S,

Journal:

PubMed ID:24843151

'Activation of the hippocampal dopamine 1-class receptors (D1R and D5R) are implicated in contextual fear conditioning (CFC). However, the specific role of the D1R versus D5R in hippocampal dependent CFC has not been investigated. Generation of D1R- and D5R-specific in situ hybridization probes showed that D1R and D5R mRNA expression ... More

Phenotypic heterogeneity and plasticity of isocortical and hippocampal astrocytes in the human brain.

Authors:Sosunov AA, Wu X, Tsankova NM, Guilfoyle E, McKhann GM, Goldman JE,

Journal:

PubMed ID:24501367

'To examine the diversity of astrocytes in the human brain, we immunostained surgical specimens of temporal cortex and hippocampus and autopsy brains for CD44, a plasma membrane protein and extracellular matrix receptor. CD44 antibodies outline the details of astrocyte morphology to a degree not possible with glial fibrillary acidic protein ... More

Neutralization of Nogo-A enhances synaptic plasticity in the rodent motor cortex and improves motor learning in vivo.

Authors:Zemmar A, Weinmann O, Kellner Y, Yu X, Vicente R, Gullo M, Kasper H, Lussi K, Ristic Z, Luft AR, Rioult-Pedotti M, Zuo Y, Zagrebelsky M, Schwab ME,

Journal:

PubMed ID:24966370

The membrane protein Nogo-A is known as an inhibitor of axonal outgrowth and regeneration in the CNS. However, its physiological functions in the normal adult CNS remain incompletely understood. Here, we investigated the role of Nogo-A in cortical synaptic plasticity and motor learning in the uninjured adult rodent motor cortex. ... More

Superficially projecting principal neurons in layer V of medial entorhinal cortex in the rat receive excitatory retrosplenial input.

Authors:Czajkowski R, Sugar J, Zhang SJ, Couey JJ, Ye J, Witter MP,

Journal:

PubMed ID:24089485

Principal cells in layer V of the medial entorhinal cortex (MEC) have a nodal position in the cortical-hippocampal network. They are the main recipients of hippocampal output and receive inputs from several cortical areas, including a prominent one from the retrosplenial cortex (RSC), likely targeting basal dendrites of layer V ... More

View all NeuroTrace™ 640/660 Deep-Red Fluorescent Nissl Stain - Solution in DMSO citations