Tampon de charge MOPS SDS (20X) NuPAGE™
Tampon de charge MOPS SDS (20X) NuPAGE™
Citations et références (1)
Invitrogen™

Tampon de charge MOPS SDS (20X) NuPAGE™

Le tampon de charge NuPAGE MOPS SDS (20X) est formulé pour la migration de protéines sur gels NuPAGE Bis-Tris. IlAfficher plus
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RéférenceQuantité
NP0001025 L
NP0001500 ml
Référence NP000102
Prix (EUR)
779,65
Online Exclusive
942,00
Économisez 162,35 (17%)
Each
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Quantité:
5 L
Prix (EUR)
779,65
Online Exclusive
942,00
Économisez 162,35 (17%)
Each
Ajouter au panier
Le tampon de charge NuPAGE MOPS SDS (20X) est formulé pour la migration de protéines sur gels NuPAGE Bis-Tris. Il est recommandé pour la séparation des protéines de dimension moyenne ou de grande dimension.

Utiliser le bon tampon pour optimiser la séparation des protéines
Les tampons de charge NuPAGE MES SDS et NuPAGE MOPS SDS peuvent tous deux être utilisés avec les gels NuPAGE Bis-Tris. Le MES possède un pKa inférieur à celui du MOPS, ce qui rend le tampon de charge MES SDS plus rapide que les tampons de charge MOPS SDS. La différence de migration ionique affecte l’empilement et entraîne une différence de plage de séparation des protéines entre ces tampons. L’utilisation du tampon MOPS permet une migration plus lente des protéines que l’utilisation du tampon MES.

Comparez les profils de migration des protéines à l’aide des tampons MES et MOPS sur gels NuPAGE Bis-Tris

Affichez tous les tampons et réactifs disponibles pour SDS-PAGE

Usage exclusivement réservé à la recherche. Ne pas utiliser pour des procédures de diagnostic.
Spécifications
Nom chimique ou matériauTampon de migration
Formule50 mM MOPS, 50 mM Tris Base, 0.1% SDS, 1 mM EDTA, pH 7.7, 0.01-0.09% N,N-dimethylformamide
Température de stockageContenu : Tampon de migration NuPAGE™ MOPS SDS (20X)
Stockage : +4°C à 25°C
Concentration20 X
Forme physiqueLiquide
Gamme de produitsNuPAGE
Quantité5 L
pH7.7
Unit SizeEach

Foire aux questions (FAQ)

Can I prepare my protein sample with the reducing agent and store it for future use?

DTT is not stable, so it must be added and the reduction performed just prior to loading your samples.

Find additional tips, troubleshooting help, and resources within our Protein Gel 1D Electrophoresis Support Center.

My LDS or SDS sample buffer precipitates when stored at 4 degrees C. Can I warm it up? Can I store it at room temperature?

Precipitation of the LDS or SDS at 4 degrees C is normal. Bring the buffer to room temperature and mix until the LDS/SDS goes into solution. If you do not want to wait for it to dissolve, you can store the sample buffer at room temperature.

Find additional tips, troubleshooting help, and resources within our Protein Gel 1D Electrophoresis Support Center.

How are Bolt gels different than NuPAGE gels?

While they are both Bis-Tris based gels, the chemistries are very different since Bolt gels are optimized for western blotting. Another key difference is the wedge well design of the Bolt gels, which allows larger sample volumes to be loaded.

Find additional tips, troubleshooting help, and resources within our Protein Gel 1D Electrophoresis Support Center.

What is the advantage of NuPAGE Gels over regular Tris-Glycine gels?

The neutral operating pH of the NuPAGE Gels and buffers provides following advantages over the Laemmli system:
-Longer shelf life of 8-12 months due to improved gel stability
-Improved protein stability during electrophoresis at neutral pH resulting in sharper band resolution and accurate results (Moos et al, 1998)
-Complete reduction of disulfides under mild heating conditions (70 degrees C for 10 min) and absence of cleavage of asp-pro bonds using the NuPAGE LDS Sample buffer (pH > 7.0 at 70 degrees C)
-Reduced state of the proteins maintained during electrophoresis and blotting of the proteins by the NuPAGE Antioxidant
Please refer to the following paper: Moos M Jr, Nguyen NY, Liu TY (1988) Reproducible High Yield Sequencing of Proteins Electrophoretically Separated and Transferred to an Inert Support. J Biol Chem 263:6005-6008.

Find additional tips, troubleshooting help, and resources within our Protein Gel 1D Electrophoresis Support Center.

Can I use NuPAGE Bis-Tris gels with NuPAGE MOPS or MES Running Buffer prepared without SDS for electrophoresis under native conditions?

We do not recommend using NuPAGE Bis-Tris gels with NuPAGE MOPS or MES Running Buffer prepared without SDS for electrophoresis under native conditions. This buffer system may generate excessive heat, resulting in poor band resolution. Further, the protein of interest may not migrate very well in a neutral pH environment if it is not charged.

Find additional tips, troubleshooting help, and resources within our Protein Electrophoresis and Western Blotting Support Center.

View all Tampon de charge MOPS SDS (20X) NuPAGE™ FAQs

Citations et références (1)

Citations et références
Abstract
Heat shock protein-70 expressed on the surface of cancer cells binds parathyroid hormone-related protein in vitro.
Authors:Grzesiak JJ, Smith KC, Chalberg C, Truong C, Burton DW, Deftos LJ, Bouvet M,
Journal:Endocrinology
PubMed ID:15878959
Recent studies have shown that the functions of PTH-related protein (PTHrP) and its derived peptides cannot be attributed solely to PTH/PTHrP receptor binding. The present study focused on the identification of other proteins that might bind PTHrP at the cell surface. Using affinity chromatography, we applied extracts of cell-surface biotinylated ... More