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Agent de réduction d’échantillons (10X) NuPAGE™
Invitrogen™

Agent de réduction d’échantillons (10X) NuPAGE™

L’agent réducteur d’échantillons NuPAGE (10X) permet de réduire les échantillons de protéines pour l’électrophorèse sur gel de protéines. Il contientAfficher plus
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RéférenceQuantité
NP000910 ml
NP0004250μl
Référence NP0009
Prix (EUR)
119,00
Each
Quantité:
10 ml
Prix (EUR)
119,00
Each
L’agent réducteur d’échantillons NuPAGE (10X) permet de réduire les échantillons de protéines pour l’électrophorèse sur gel de protéines. Il contient 500 mM de dithiothréitol (DTT), à une concentration 10X, sous une forme liquide stabilisée.

Affichez tous les tampons et réactifs disponibles pour SDS-PAGE

Usage exclusivement réservé à la recherche. Ne pas utiliser pour des procédures de diagnostic.
Spécifications
Concentration500 mM
Quantité10 ml
Durée de conservation6 mois
Conditions d’expéditionGlace humide
Gamme de produitsNuPAGE
Type de produitAgent réducteur d’échantillons
Unit SizeEach
Contenu et stockage
L’agent réducteur d’échantillons NuPAGE™ (500 mM de dithiothréitol) doit être conservé à 4°C. Garanti stable pendant 6 mois, sauf indication contraire mentionnée dans la documentation produit.

Foire aux questions (FAQ)

Can I prepare my protein sample with the reducing agent and store it for future use?

DTT is not stable, so it must be added and the reduction performed just prior to loading your samples.

Find additional tips, troubleshooting help, and resources within our Protein Gel 1D Electrophoresis Support Center.

My LDS or SDS sample buffer precipitates when stored at 4 degrees C. Can I warm it up? Can I store it at room temperature?

Precipitation of the LDS or SDS at 4 degrees C is normal. Bring the buffer to room temperature and mix until the LDS/SDS goes into solution. If you do not want to wait for it to dissolve, you can store the sample buffer at room temperature.

Find additional tips, troubleshooting help, and resources within our Protein Gel 1D Electrophoresis Support Center.

How are Bolt gels different than NuPAGE gels?

While they are both Bis-Tris based gels, the chemistries are very different since Bolt gels are optimized for western blotting. Another key difference is the wedge well design of the Bolt gels, which allows larger sample volumes to be loaded.

Find additional tips, troubleshooting help, and resources within our Protein Gel 1D Electrophoresis Support Center.

What is the advantage of NuPAGE Gels over regular Tris-Glycine gels?

The neutral operating pH of the NuPAGE Gels and buffers provides following advantages over the Laemmli system:
-Longer shelf life of 8-12 months due to improved gel stability
-Improved protein stability during electrophoresis at neutral pH resulting in sharper band resolution and accurate results (Moos et al, 1998)
-Complete reduction of disulfides under mild heating conditions (70 degrees C for 10 min) and absence of cleavage of asp-pro bonds using the NuPAGE LDS Sample buffer (pH > 7.0 at 70 degrees C)
-Reduced state of the proteins maintained during electrophoresis and blotting of the proteins by the NuPAGE Antioxidant
Please refer to the following paper: Moos M Jr, Nguyen NY, Liu TY (1988) Reproducible High Yield Sequencing of Proteins Electrophoretically Separated and Transferred to an Inert Support. J Biol Chem 263:6005-6008.

Find additional tips, troubleshooting help, and resources within our Protein Gel 1D Electrophoresis Support Center.

Where do I find buffer recipes for your precast protein gels?

The formulations of buffers for our precast protein gels can be found at this link: https://www.thermofisher.com/us/en/home/life-science/protein-biology/protein-gel-electrophoresis/protein-electrophoresis-buffers-reagents.html

Find additional tips, troubleshooting help, and resources within our Protein Electrophoresis and Western Blotting Support Center.

View all Agent de réduction d’échantillons (10X) NuPAGE™ FAQs