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Kit de tampons MOPS SDS NuPAGE™ (pour gels Bis-Tris)
Citations et références (1)
Invitrogen™

Kit de tampons MOPS SDS NuPAGE™ (pour gels Bis-Tris)

Le kit de tampons NuPAGE MOPS SDS est conçu pour la séparation des protéines de taille moyenne à grande surAfficher plus
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RéférenceQuantité
NP00501 kit
Référence NP0050
Prix (EUR)
196,00
Each
Quantité:
1 kit
Prix (EUR)
196,00
Each
Le kit de tampons NuPAGE MOPS SDS est conçu pour la séparation des protéines de taille moyenne à grande sur les gels NuPAGE Bis-Tris et comprend les tampons suivants :
• Tampon de charge NuPAGE MOPS SDS (20X, 500 ml, n° de catalogue NP0001)
• Agent réducteur d’échantillons NuPAGE (10X, 250 µL, n° de catalogue NP0004)
• Antioxydant NuPAGE (n° de catalogue NP0005)
• Tampon d’échantillon NuPAGE LDS (4 X, 10 ml, n° de catalogue N° NP0007)

Comparez les profils de migration des protéines à l’aide de MES et de MOPs sur les gels NuPAGE Bis-Tris

Voir tous les tampons et réactifs disponibles pour SDS-PAGE
Usage exclusivement réservé à la recherche. Ne pas utiliser pour des procédures de diagnostic.
Spécifications
Type de gelGel SDS PAGE
Quantité1 kit
Conditions d’expéditionTempérature ambiante
Gel CompatibilityGel NuPAGE™ Bis-Tris
Gamme de produitsNuPAGE
Type de produitTampon de migration
Unit SizeEach
Contenu et stockage
Contient
• un tampon de charge NuPAGE™ MOPS SDS (20X, 500 ml)
•™ Un agent réducteur d’échantillons NuPAGE (10X, 250 µl) contenant 500 mM de dithiothréitol
•Un antioxydant (15 ml) NuPAGE™
• Un tampon d’échantillon NuPAGE™ LDS (10 ml)
Conserver chacun à 4°C. Chauffer™ le tampon d’échantillon NuPAGE LDS à 25°C avant utilisation.

Foire aux questions (FAQ)

Can I prepare my protein sample with the reducing agent and store it for future use?

DTT is not stable, so it must be added and the reduction performed just prior to loading your samples.

Find additional tips, troubleshooting help, and resources within our Protein Gel 1D Electrophoresis Support Center.

My LDS or SDS sample buffer precipitates when stored at 4 degrees C. Can I warm it up? Can I store it at room temperature?

Precipitation of the LDS or SDS at 4 degrees C is normal. Bring the buffer to room temperature and mix until the LDS/SDS goes into solution. If you do not want to wait for it to dissolve, you can store the sample buffer at room temperature.

Find additional tips, troubleshooting help, and resources within our Protein Gel 1D Electrophoresis Support Center.

How are Bolt gels different than NuPAGE gels?

While they are both Bis-Tris based gels, the chemistries are very different since Bolt gels are optimized for western blotting. Another key difference is the wedge well design of the Bolt gels, which allows larger sample volumes to be loaded.

Find additional tips, troubleshooting help, and resources within our Protein Gel 1D Electrophoresis Support Center.

What is the advantage of NuPAGE Gels over regular Tris-Glycine gels?

The neutral operating pH of the NuPAGE Gels and buffers provides following advantages over the Laemmli system:
-Longer shelf life of 8-12 months due to improved gel stability
-Improved protein stability during electrophoresis at neutral pH resulting in sharper band resolution and accurate results (Moos et al, 1998)
-Complete reduction of disulfides under mild heating conditions (70 degrees C for 10 min) and absence of cleavage of asp-pro bonds using the NuPAGE LDS Sample buffer (pH > 7.0 at 70 degrees C)
-Reduced state of the proteins maintained during electrophoresis and blotting of the proteins by the NuPAGE Antioxidant
Please refer to the following paper: Moos M Jr, Nguyen NY, Liu TY (1988) Reproducible High Yield Sequencing of Proteins Electrophoretically Separated and Transferred to an Inert Support. J Biol Chem 263:6005-6008.

Find additional tips, troubleshooting help, and resources within our Protein Gel 1D Electrophoresis Support Center.

Can beta-mercaptoethanol (BME) be used rather than DTT as the reducing agent in the NuPAGE LDS Sample Buffer?

Either BME or DTT can be used in the NuPAGE LDS Sample Buffer.

Make sure that a fresh solution of BME is used. FINAL concentration:

DTT 50-100 mM

BME 2-5%

Find additional tips, troubleshooting help, and resources within our Protein Electrophoresis and Western Blotting Support Center.

View all Kit de tampons MOPS SDS NuPAGE™ (pour gels Bis-Tris) FAQs

Citations et références (1)

Citations et références
Abstract
Heat shock protein-70 expressed on the surface of cancer cells binds parathyroid hormone-related protein in vitro.
Authors:Grzesiak JJ, Smith KC, Chalberg C, Truong C, Burton DW, Deftos LJ, Bouvet M,
Journal:Endocrinology
PubMed ID:15878959
Recent studies have shown that the functions of PTH-related protein (PTHrP) and its derived peptides cannot be attributed solely to PTH/PTHrP receptor binding. The present study focused on the identification of other proteins that might bind PTHrP at the cell surface. Using affinity chromatography, we applied extracts of cell-surface biotinylated ... More