Iodure de propidium - solution aqueuse à 1,0 mg/ml
Invitrogen™

Iodure de propidium - solution aqueuse à 1,0 mg/ml

L’iodure de propidium (IP) est un contre-colorant rouge fluorescent, chromosomique et nucléaire, courant Étant donné que l’iodure de propidium n’estAfficher plus
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RéférenceQuantité
P356610 ml
Référence P3566
Prix (EUR)
185,00
Each
Quantité:
10 ml
Prix (EUR)
185,00
Each
L’iodure de propidium (IP) est un contre-colorant rouge fluorescent, chromosomique et nucléaire, courant Étant donné que l’iodure de propidium n’est pas perméant aux cellules vivantes, il est également couramment utilisé pour détecter des cellules mortes dans une population.

L’IP est lié à l’ADN en s’intercalant entre les bases avec une préférence de séquence faible ou nulle. Dans une solution aqueuse, le colorant enregistre des maxima d’excitation/émission de 493/636 nm. Après la liaison du colorant, sa fluorescence est accrue de 20 à 30 fois, le maximum d’excitation de la fluorescence passe d’∼ 30– 40 nm au rouge et le maximum d’émission de la fluorescence passe de ∼ 15 nm au bleu, ce qui se solde par une excitation maximale à 535 nm et une émission maximale de la fluorescence à 617 nm.

L’IP est couramment utilisé en microscopie par fluorescence, en microscopie confocale à balayage laser, en cytométrie de flux et en fluorimétrie.

En savoir plus sur l’iodure de propidium et les produits contenant de l’iodure de propidium
Usage exclusivement réservé à la recherche. Ne pas utiliser pour des procédures de diagnostic.
Spécifications
Méthode de détectionFluorescent, Fluorescence
Type de colorantIodure de propidium
FormeSolution
Quantité10 ml
Conditions d’expéditionTempérature ambiante, Température ambiante
Localisation subcellulaireCytoplasme & cytosol, Cytoplasme & cytosol
Emission533 / 617
À utiliser avec (application)Test de viabilité
À utiliser avec (équipement)Microscope à fluorescence, Cytomètre en flux
Type de produitIodure de propidium
Unit SizeEach
Contenu et stockage
Contient 1 flacon d’iodure de propidium (solution aqueuse à 1,0 mg/ml).

Conserver au réfrigérateur (2–8 °C) et à l’abri de la lumière.

Foire aux questions (FAQ)

I have a LIVE/DEAD BacLight Bacterial Viability kit that has SYTO 9 and propidium iodide in it. Will I be able to stain eukaryotic cells that have engulfed bacteria and determine if the bacteria are alive or dead using this kit?

Unfortunately, no. SYTO 9 will label the nuclei of live or dead cells, including the eukaryotic cells. Propidium iodide is cell impermeant, and will only enter dead cells. If the eukaryotic cells are dead, they will label with propidium iodide as well. If the eukaryotic cells are alive, propidium iodide will not be able to enter and thus will not label the bacteria inside, whether the bacteria are alive or dead. We are not aware of any way to do a viability assay of bacteria once they have been engulfed by cells.

Find additional tips, troubleshooting help, and resources within our Cell Analysis Support Center.

Is propidium iodide (PI) fixable with glutaraldehyde or paraformaldehyde (PFA)?

PI is not fixable with glutaraldehyde or PFA. Both reagents fix by crosslinking amines. PI and other nucleic acid stains do not inherently bind covalently to nucleic acids and these fixatives do not crosslink the dyes to nucleic acids.
The one fixable nucleic acid stain is Ethidium Monoazide Bromide (EMA), Cat no. E1374); it covalently binds to nucleic acids upon activation by exposure to light.

Find additional tips, troubleshooting help, and resources within our Cell Analysis Support Center.

Citations et références (1088)

Citations et références
Abstract
Signaling through MHC class II molecules blocks CD95-induced apoptosis.
Authors:Catlett IM,Xie P,Hostager BS,Bishop GA
Journal:Journal of immunology (Baltimore, Md. : 1950)
PubMed ID:11342618
Glutamate and non-glutamate receptor mediated toxicity caused by oxygen and glucose deprivation in organotypic hippocampal cultures.
Authors:Newell DW, Barth A, Papermaster V, Malouf AT
Journal:J Neurosci
PubMed ID:7472521
In vitro ischemia models have utilized oxygen, or oxygen and glucose deprivation to simulate ischemic neuronal injury. Combined oxygen and glucose deprivation can induce neuronal damage which is in part mediated through NMDA receptors. Severe oxygen deprivation alone however can cause neuronal injury which is not NMDA mediated. We tested ... More
Identification and characterization of two subpopulations of Encephalitozoon intestinalis.
Authors:Hoffman RM, Marshall MM, Polchert DM, Jost BH
Journal:Appl Environ Microbiol
PubMed ID:12902292
Microsporidia are obligate intracellular protozoa that have been shown to be pathogenic to most living creatures. The development of in vitro cell culture propagation methods has provided researchers with large numbers of spores and facilitated the study of these organisms. Here, we describe heterogeneity within cell culture-propagated Encephalitozoon intestinalis suspensions. ... More
Autoantigens targeted in systemic lupus erythematosus are clustered in two populations of surface structures on apoptotic keratinocytes.
Authors:Casciola-Rosen LA, Anhalt G, Rosen A
Journal:J Exp Med
PubMed ID:7511686
'Systemic lupus erythematosus is a multisystem autoimmune disease in which the autoantibody response targets a variety of autoantigens of diverse subcellular location. We show here that these autoantigens are clustered in two distinct populations of blebs at the surface of apoptotic cells. The population of smaller blebs contains fragmented endoplasmic ... More
Caspase activation contributes to delayed death of heat-stressed striatal neurons.
Authors:White MG, Emery M, Nonner D, Barrett JN
Journal:J Neurochem
PubMed ID:14622126
'Hyperthermia can contribute to brain damage both during development and post-natally. We used rat embryonic striatal neurons in culture to study mechanisms underlying hyperthermia-induced neuronal death. Heat stress at 43 degrees C for 2 h produced no obvious signs of damage during the first 12 h after the stress, but ... More