Milieu de montage anti-atténuation SlowFade™ Gold
Milieu de montage anti-atténuation SlowFade™ Gold
Le produit réel peut varier
Milieu de montage anti-atténuation SlowFade™ Gold
Milieu de montage anti-atténuation SlowFade™ Gold
Citations et références (17)
Invitrogen™

Milieu de montage anti-atténuation SlowFade™ Gold

Green features
Le milieu de montage anti-atténuation SlowFade Gold est un milieu de montage liquide appliqué directement sur les échantillons de tissusAfficher plus
Have Questions?
Modifier l'affichagebuttonViewtableView
RéférenceQuantité
S369375 x 2 ml
S369402 mL
S3693610 mL
Référence S36937
Prix (EUR)
308,65
Online Exclusive
335,00
Économisez 26,35 (8%)
Each
Ajouter au panier
Quantité:
5 x 2 ml
Prix (EUR)
308,65
Online Exclusive
335,00
Économisez 26,35 (8%)
Each
Ajouter au panier
Le milieu de montage anti-atténuation SlowFade Gold est un milieu de montage liquide appliqué directement sur les échantillons de tissus ou cellules marqués par fluorescence présents sur les lames de microscope. Il contient des composants chimiques conçus pour protéger les colorants fluorescents de la décoloration (photoblanchiment) lors d’expériences de microscopie par fluorescence. Le milieu de montage anti-atténuation SlowFade Gold est à base de glycérol et ne nécessite aucun mélange ou traitement, ce qui le rend idéal pour une visualisation immédiate de l’échantillon. Il est livré prêt à l’emploi—il suffit dʼappliquer une goutte sur lʼéchantillon, dʼajouter une lamelle et de prendre des images. Il est disponible avec ou sans colorant nucléaire DAPI.

Principaux attributs :
• Protège les colorants contre la décoloration pendant l’imagerie
• Liquide prêt à l’emploi, idéal pour une visualisation immédiate des échantillons
• Les échantillons montés sont stables pendant plusieurs semaines
• Préserve l’intensité du signal—Peu ou pas d’extinction
• Idéal pour les colorants Alexa Fluor

Sélectionnez le réactif de montage ou optique anti-atténuation qui correspond à vos besoins d’imagerie de fluorescence ›

Le milieu de montage anti-atténuation SlowFade Gold n’est pas recommandé pour le montage dʼéchantillons contenant des protéines fluorescentes, comme GFP. Pour une protection supérieure des protéines et des colorants fluorescents contre lʼatténuation dans les cellules de culture ou les coupes de tissus minces, il est recommandé dʼutiliser le milieu de montage anti-atténuation SlowFade Diamond. Pour la haute résolution ou pour imager des tissus plus épais ou des cultures cellulaires 3D avec une profondeur focale de 0–500 µm, essayez le milieu de montage anti-atténuation en verre SlowFade.

Sélectionnez le meilleur réactif anti-atténuation SlowFade pour vos besoins expérimentaux ›

Usage exclusivement réservé à la recherche. Ne pas utiliser pour des procédures de diagnostic.

Spécifications
Concept écologiqueEmballage moins dangereux et durable
Gamme de produitsSlowFade
Quantité5 x 2 ml
Conditions d’expéditionTempérature ambiante
À utiliser avec (équipement)Microscope
FormeLiquide
Type de produitMilieu de montage anti-atténuation
Type de solutionSolution anti-atténuation
Unit SizeEach
Contenu et stockage
Le stockage à température ambiante est recommandé, mais il peut également être conservé congelé (-5 à -30°C). Conserver à l’abri de la lumière.

Foire aux questions (FAQ)

What is the difference between ProLong and SlowFade antifade reagents?

Our ProLong antifade reagents dispense as a liquid that will solidify upon the evaporation of water. SlowFade antifade reagents remain liquid. If you are going to image right away and then dispose of your sample, you do not need a mountant that hardens, such as the SlowFade reagents. If you wish to archive your slide for more than a day, you will want a mounting medium that hardens (or “cures”). This hardening will limit the off-rates of various dye-conjugated antibodies and provides a better refractive index. Also, there will be a lower diffusion rate of free radicals, limiting photobleaching.

Find additional tips, troubleshooting help, and resources within our Cell Analysis Support Center.

Some antifade mounting media stay as liquid whereas others harden. What is the benefit of having one that hardens?

If you are going to image right away and then dispose of your sample, you probably want a mountant that does not harden. If you wish to archive your slide for more than a day, you want a mountant that hardens (or "cures"). This hardening will slow or prevent off-rate of your dye or conjugate and often produces a better refractive index. Secondary sealing is usually not necessary. Also there will be lower diffusion of free radicals, thus limiting photobleaching.

Find additional tips, troubleshooting help, and resources within our Cell Analysis Support Center.

I do not have epoxy or VALAP to seal the coverslip. Do you offer an alternative coverslip sealant?

We offer ProLong Coverslip Sealant (Cat. No. P56128) that can be used to seal the edges of the coverslip and is compatible with both curing and non-curing mountant. The sealant is easy to apply and is brushed on after the mountant has cured, for long preservation of slides. The product page can be found here.

When using a hard-curing mountant, such as ProLong Antifade Mountant, make sure the mountant is fully cured before applying ProLong Coverslip Sealant. Since this sealant can seal moisture under the coverslip, it can interfere with the curing process.

When using a non-curing mountant, such as SlowFade Antifade Mountants, sealing can take place immediately after mounting.

Find additional tips, troubleshooting help, and resources within our Cell Analysis Support Center.

Does Slowfade Antifade Mountant require using a nail polish for sealing?

No. Samples mounted with SlowFade mountants need not be sealed; they are intended for immediate viewing. The coverslip may be anchored (to prevent movement while viewing) by applying molten paraffin to three or four spots around the edge of the coverslip.

Find additional tips, troubleshooting help, and resources within our Cell Analysis Support Center.

Citations et références (17)

Citations et références
Abstract
Reactive oxygen species production via NADPH oxidase mediates TGF-beta-induced cytoskeletal alterations in endothelial cells.
Authors:Hu T, Ramachandrarao SP, Siva S, Valancius C, Zhu Y, Mahadev K, Toh I, Goldstein BJ, Woolkalis M, Sharma K
Journal:Am J Physiol Renal Physiol
PubMed ID:16159901
'Cytoskeletal alterations in endothelial cells have been linked to nitric oxide generation and cell-cell interactions. Transforming growth factor (TGF)-beta has been described to affect cytoskeletal rearrangement in numerous cell types; however, the underlying pathway is unclear. In the present study, we found that human umbilical vein endothelial cells (HUVEC) have ... More
Identification and characterization of small molecules that inhibit intracellular toxin transport.
Authors:Saenz JB, Doggett TA, Haslam DB
Journal:Infect Immun
PubMed ID:17576758
'Shiga toxin (Stx), cholera toxin (Ctx), and the plant toxin ricin are among several toxins that reach their intracellular destinations via a complex route. Following endocytosis, these toxins travel in a retrograde direction through the endosomal system to the trans-Golgi network, Golgi apparatus, and endoplasmic reticulum (ER). There the toxins ... More
Rac-mediated macropinocytosis is a critical route for naked plasmid DNA transfer in mice.
Authors:Fumoto S, Nishi J, Ishii H, Wang X, Miyamoto H, Yoshikawa N, Nakashima M, Nakamura J, Nishida K,
Journal:Mol Pharm
PubMed ID:19492848
We have recently discovered the potential for in vivo naked plasmid DNA (pDNA) transfer into gastric serosal surface cells in mice. As pDNA are huge molecules, the mechanism of gene transfer without carriers and physical forces is of great biological interest. The endocytic route for naked pDNA transfer into gastric ... More
Programmable in situ amplification for multiplexed imaging of mRNA expression.
Authors:Choi HM, Chang JY, Trinh le A, Padilla JE, Fraser SE, Pierce NA,
Journal:Nat Biotechnol
PubMed ID:21037591
In situ hybridization methods enable the mapping of mRNA expression within intact biological samples. With current approaches, it is challenging to simultaneously map multiple target mRNAs within whole-mount vertebrate embryos, representing a significant limitation in attempting to study interacting regulatory elements in systems most relevant to human development and disease. ... More
A protocol for dissecting Drosophila melanogaster brains for live imaging or immunostaining.
Authors:Wu JS, Luo L
Journal:Nat Protoc
PubMed ID:17487202
This protocol describes a basic method for dissection and immunofluorescence staining of the Drosophila brain at various developmental stages. The Drosophila brain has become increasingly useful for studies of neuronal wiring and morphogenesis in combination with techniques such as the 'mosaic analysis with a repressible cell marker' (MARCM) system, where ... More
View all Milieu de montage anti-atténuation SlowFade™ Gold citations