pTK-Green Renilla Luc Vector for Luciferase Assays
Thermo Scientific™
pTK-Green Renilla Luc Vector for Luciferase Assays
The Thermo Scientific pCMV-Green Renilla Luc vector is a derivative of pMCS-Green Renilla Luc. It contains the luciferase gene underRead more
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Catalog Number
Quantity
16154
10 μg
Catalog number 16154
Price (KRW)
148,000
Online offer
Ends: 31-Dec-2025
173,000
Save 25,000 (14%)
Each
Add to cart
Quantity:
10 μg
Price (KRW)
148,000
Online offer
Ends: 31-Dec-2025
173,000
Save 25,000 (14%)
Each
Add to cart
The Thermo Scientific pCMV-Green Renilla Luc vector is a derivative of pMCS-Green Renilla Luc. It contains the luciferase gene under the control of the Herpes Simplex Virus (HSV) thymidine kinase (TK) promoter, creating a constitutive expression vector that can be used as a normalization control to account for experimental variation in combination with other reporters.
Features of the pTK-Green Renilla Luc vector:
• Weak (TK) constitutive promoter for co-transfection and normalization • Intracellular Green Renilla luciferase gene, optimized for high expression in mammalian systems • Transcription termination site (Ter), Lac operator (Lac O1), and transcriptional pause site (TPS) used to minimize background by reducing transcriptional read-through • Both puromycin (Pur) and ampicillin (Amp) markers for drug selection in mammalian and bacterial cells, respectively • High-copy pUC bacterial DNA replication origin
For Research Use Only. Not for use in diagnostic procedures.
Specifications
Cloning MethodRestriction Enzyme/MCS
Delivery TypeTransfection
DescriptionpTK-Green Renilla Luc Vector
For Use With (Application)Reporter Assays
Product LinePierce
Quantity10 μg
Reporter GeneGreen Renilla Luciferase
Selection Agent (Eukaryotic)Puromycin
PromoterTK
TypeLuc Vector
Unit SizeEach
Contents & Storage
Store in original container protected from direct sunlight in a dry, cool and well-ventilated area, between the following temperatures: -70 to -20°C.
Frequently asked questions (FAQs)
What options do you offer for luciferase vectors?
There are three vectors available for each expressed luciferase; pMCS, pCMV, and pTK. We recommend using pMCS to measure the activity of the reporter, and pCMV, and pTK as positive control vectors. For high level constitutive expression of Luciferase, we recommend using the CMV (Cytomegalovirus) promoter vector and for low level constitutive expression of luciferase, we recommend using the pTK (thymidine kinase) promoter vector.
Gaussia, Gaussia-Dura, and Cypridina Luciferase are stable in the culture media for greater than 24 hours. Generally, after transfection of CMV driven constructs, we see ever increasing secreted signal between 24 and 72 hours.
The earliest detectable signal will depend on the strength of the promoter. In general, with a strong promoter (such as CMV), significant signal over background can be seen in as little as 20 minutes. However, with a weaker inducible promoter, significant signal over background may take 1-2 hours after induction.
Do you still offer quantitative IP (qIP) Luciferase assay kits?
Our qIP Luciferase assay kits have been discontinued but we do carry epitope-tagged (HA or c-Myc) and Tluc (TurboLuc luciferase)-tagged mammalian expression vectors, and qIP protein interaction assay reagents for these assay kits.