Thermo Scientific™

pCMV-Green Renilla Luc Vector for Luciferase Assays

Catalog number:  16153

 Related applications: Protein Assays and Analysis | Protein Expression

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The Thermo Scientific pCMV-Green Renilla Luc vector is a derivative of pMCS-Green Renilla Luc. It contains the luciferase gene under the control of the CMV (Cytomegalovirus) promoter, creating a constitutive expression vector that can be used as a normalization control to account for experimental variation in combination with other reporters.

Features of the pCMV-Green Renilla Luc vector:

• Strong (CMV) constitutive promoter for co-transfection and normalization
• Intracellular Green Renilla luciferase gene, optimized for high expression in mammalian systems
• Transcription termination site (Ter), Lac operator (Lac O1), and transcriptional pause site (TPS) used to minimize background by reducing transcriptional read-through
• Both puromycin (Pur) and ampicillin (Amp) markers for drug selection in mammalian and bacterial cells, respectively
• High-copy pUC bacterial DNA replication origin

More Product Data
Luciferase assays in hard-to-transfect Jurkat cells

Related Products
pMCS-Green Renilla Luc Vector for Luciferase Assays
pTK-Green Renilla Luc Vector for Luciferase Assays
For Research Use Only. Not for use in diagnostic procedures.


Promoter: CMV
Key Function: Reporter Assays
Reporter: Green Renilla Luciferase
Delivery Method: Transfection
Cloning Method: Restriction Enzyme ⁄ MCS
Selection Agent (Eukaryotic): Puromycin
Product Line: Pierce™
Product Size: 10 µg

Contents & storage

Store in original container protected from direct sunlight in a dry, cool and well-ventilated area, between the following temperatures: -70 to -20°C.


Manuals & protocols

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