Thermo Scientific™
pMCS-Green Renilla Luc Vector for Luciferase Assays
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Description
The Thermo Scientific pMCS-Renilla Luc vector is a multiple cloning site plasmid to designed to accept a promoter sequence for study of gene regulation using the intracellular Renilla luciferase reporter.Features of the pMCS-Renilla Luc vector:
• Intracellular Green Renilla luciferase gene, optimized for high expression in mammalian systems
• Multiple cloning site (MCS) provides versatility for transfer of regulatory elements from one plasmid to another
• Transcription termination site (Ter), Lac operator (Lac O1), and transcriptional pause site (TPS) used to minimize background by reducing transcriptional read-through
• Both puromycin (Pur) and ampicillin (Amp) markers for drug selection in mammalian and bacterial cells, respectively
• High-copy pUC bacterial DNA replication origin
The Green Renilla Luc Vectors contain a gene cloned from the sea pansy, Renilla reniformis. The gene encodes a bioluminescent Green Renilla luciferase (36kDa). The pMCS vector contains a multiple cloning site for cloning a promoter to study its regulatory potential.
These vectors are subject to a limited use label license.
More Product Data
• Luciferase assays in hard-to-transfect Jurkat cells
Related Products
pCMV-Green Renilla Luc Vector for Luciferase Assays
pTK-Green Renilla Luc Vector for Luciferase Assays
For Research Use Only. Not for use in diagnostic procedures.
Specifications
| Key Function: | Reporter Assays |
|---|---|
| Reporter: | Green Renilla Luciferase |
| Delivery Method: | Transfection |
| Cloning Method: | Restriction Enzyme ⁄ MCS |
| Selection Agent (Eukaryotic): | Puromycin |
| Product Line: | Pierce™ |
| Product Size: | 10 µg |
Contents & storage
Store in original container protected from direct sunlight in a dry, cool and well-ventilated area, between the following temperatures: -70 to -20°C.
