pMCS-Green Renilla Luc Vector for Luciferase Assays

Description

The Thermo Scientific pMCS-Renilla Luc vector is a multiple cloning site plasmid to designed to accept a promoter sequence for study of gene regulation using the intracellular Renilla luciferase reporter.

Features of the pMCS-Renilla Luc vector:

• Intracellular Green Renilla luciferase gene, optimized for high expression in mammalian systems
• Multiple cloning site (MCS) provides versatility for transfer of regulatory elements from one plasmid to another
• Transcription termination site (Ter), Lac operator (Lac O1), and transcriptional pause site (TPS) used to minimize background by reducing transcriptional read-through
• Both puromycin (Pur) and ampicillin (Amp) markers for drug selection in mammalian and bacterial cells, respectively
• High-copy pUC bacterial DNA replication origin

The Green Renilla Luc Vectors contain a gene cloned from the sea pansy, Renilla reniformis. The gene encodes a bioluminescent Green Renilla luciferase (36kDa). The pMCS vector contains a multiple cloning site for cloning a promoter to study its regulatory potential.

These vectors are subject to a limited use label license.

More Product Data
• Luciferase assays in hard-to-transfect Jurkat cells

Related Products
pCMV-Green Renilla Luc Vector for Luciferase Assays
pTK-Green Renilla Luc Vector for Luciferase Assays

Figures

Specifications

Key Function:	Reporter Assays
Reporter:	Green Renilla Luciferase
Delivery Method:	Transfection
Cloning Method:	Restriction Enzyme ⁄ MCS
Selection Agent (Eukaryotic):	Puromycin
Product Line:	Pierce™
Product Size:	10 µg

Contents & storage