FUN™ 1 Cell Stain
FUN™ 1 Cell Stain
Citations & References (47)
Invitrogen™

FUN™ 1 Cell Stain

FUN™ 1 is a unique two-color fluorescent viability probe for yeast and fungi. The FUN™ 1 stain passively diffuses intoRead more
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Catalog NumberQuantity
F7030100 μL
Catalog number F7030
Price (KRW)
398,000
온라인 행사
Ends: 31-Dec-2025
468,000
Save 70,000 (15%)
Each
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Quantity:
100 μL
Price (KRW)
398,000
온라인 행사
Ends: 31-Dec-2025
468,000
Save 70,000 (15%)
Each
Add to cart
FUN™ 1 is a unique two-color fluorescent viability probe for yeast and fungi. The FUN™ 1 stain passively diffuses into a variety of cell types and initially stains the cytoplasm with a diffusely distributed green fluorescence. However, in several common species of yeast and fungi, subsequent processing of the dye by live cells results in the formation of distinct vacuolar structures with compact form that exhibit a striking red fluorescence, accompanied by a reduction in the green cytoplasmic fluorescence. Formation of the intravacuolar structures requires both plasma membrane integrity and metabolic capability. Dead cells fluoresce bright yellow-green, with no discernable red structures.

Learn more about ion indicators including calcium, potassium, pH, and membrane potential indicators ›
For Research Use Only. Not for use in diagnostic procedures.
Specifications
Cell TypeFungal Cells, Yeast Cells
Concentration10 mM
Detection MethodFluorescence
Dye TypeOther Label(s) or Dye(s)
FormatTube(s), Slide(s)
Quantity100 μL
Shipping ConditionRoom Temperature
For Use With (Equipment)Fluorescence Microscope, Flow Cytometer
Product LineFUN
Product TypeStain
Unit SizeEach
Contents & Storage
Store in freezer -5°C to -30°C and protect from light.

Frequently asked questions (FAQs)

Can FUN 1 stained cells be examined by flow cytometry?

Yes. Use a 488 nm laser line and standard FITC and PE channels for two-color detection of green (dead/metabolically inactive cells) and red (live, metabolically active cells) emission.

Find additional tips, troubleshooting help, and resources within our Cell Analysis Support Center.

Live, metabolically active fungi transport FUN 1 into vacuoles to give a red-shifted fluorescence versus green/yellow fluorescence in the nucleus and cytoplasm of dead or metabolically-inactive cells. Is this a reliable indicator of fungal viability?

No. FUN 1 accumulates into vacuoles by an unknown transport pathway, but any mutants/ recombinant cells or experimental treatments that result in a deficiency or block in vesicle-mediate transport into vacuoles may result in cells that do not have red vacuoles, even though the cells are live and metabolically active. For more information see J Microbiol Methods 78:208 (2009).

Find additional tips, troubleshooting help, and resources within our Cell Analysis Support Center.

Citations & References (47)

Citations & References
Abstract
SOD2 functions downstream of Sch9 to extend longevity in yeast.
Authors:Fabrizio P, Liou LL, Moy VN, Diaspro A, Valentine JS, Gralla EB, Longo VD
Journal:Genetics
PubMed ID:12586694
'Signal transduction pathways inactivated during periods of starvation are implicated in the regulation of longevity in organisms ranging from yeast to mammals, but the mechanisms responsible for life-span extension are poorly understood. Chronological life-span extension in S. cerevisiae cyr1 and sch9 mutants is mediated by the stress-resistance proteins Msn2/Msn4 and ... More
Early events in macrophage killing of Aspergillus fumigatus conidia: new flow cytometric viability assay.
Authors:Marr KA, Koudadoust M, Black M, Balajee SA
Journal:Clin Diagn Lab Immunol
PubMed ID:11687470
'Detailed investigations of macrophage phagocytosis and killing of Aspergillus fumigatus conidia have been limited by technical difficulties in quantifying fungal uptake and viability. In order to study early events in cell pathogen ingestion and killing, we developed a new flow cytometry assay that utilizes the fungus-specific viability dye FUN-1. Metabolically ... More
Human immature dendritic cells efficiently bind and take up secretory IgA without the induction of maturation.
Authors:Heystek HC, Moulon C, Woltman AM, Garonne P, van Kooten C
Journal:J Immunol
PubMed ID:11751952
'Immature dendritic cells (DC) reside in peripheral tissues, where they pick up and process incoming pathogens via scavenger receptors or FcR such as FcgammaR and FcepsilonR. At mucosal surfaces, IgA is the main Ig to protect the body from incoming pathogens. In addition, DC are present in high numbers at ... More
In vitro growth and analysis of Candida biofilms.
Authors:Chandra J, Mukherjee PK, Ghannoum MA,
Journal:Nat Protoc
PubMed ID:19180075
'Evaluation of fungal biofilm formation can be performed using several techniques. In this protocol, we describe methods used to form Candida biofilms on three different medical device substrates (denture strips, catheter disks and contact lenses) to quantify them and to evaluate their architecture and drug susceptibility. Biofilm formation involves adhesion ... More
Viruses activate a genetically conserved cell death pathway in a unicellular organism.
Authors:Ivanovska I, Hardwick JM
Journal:J Cell Biol
PubMed ID:16061692
'Given the importance of apoptosis in the pathogenesis of virus infections in mammals, we investigated the possibility that unicellular organisms also respond to viral pathogens by activating programmed cell death. The M1 and M2 killer viruses of Saccharomyces cerevisiae encode pore-forming toxins that were assumed to kill uninfected yeast cells ... More
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