SuperScript™ III Platinum™ One-Step qRT-PCR Kit w/ROX
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Invitrogen™

SuperScript™ III Platinum™ One-Step qRT-PCR Kit w/ROX

The SuperScript III Platinum One-Step qRT-PCR Kit’s proven formula delivers reliable, accurate target detection when it matters most. The convenient자세히 알아보기
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카탈로그 번호반응 수
11745500500 Reactions
11745100100 Reactions
카탈로그 번호 11745500
제품 가격(KRW)
3,776,000
Each
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반응 수:
500 Reactions
제품 가격(KRW)
3,776,000
Each
카트에 추가하기
The SuperScript III Platinum One-Step qRT-PCR Kit’s proven formula delivers reliable, accurate target detection when it matters most. The convenient one-step format and consistent performance make it ideal for a number of gene expression applications, including infectious disease surveillance. The kit has been validated by several global public health organizations and has long been the choice of the US CDC for infectious disease surveillance.

Features of the SuperScript III Platinum One-Step qRT-PCR Kit include:
• SuperScript III RT for higher temperature cDNA synthesis when addressing difficult RNA secondary structure
• Platinum Taq DNA Polymerase with hot-start technology for improved specificity
• LUX fluorogenic primers and dual-labeled fluorogenic probes for effective detection performance
• ROX reference dye pre-mixed at optimal concentration for Applied Biosystems instruments
• Validated performance on multiple real-time instrument platforms, including rotor-based systems

SuperScript III Platinum One-Step qRT-PCR Kits come in multiple configurations:
SuperScript III Platinum One-Step qRT-PCR Kit with ROX passive reference (this page)
SuperScript III Platinum One-Step qRT-PCR Kit
SuperScript III Platinum SYBR Green One-Step qRT-PCR Kit
SuperScript III Platinum SYBR Green One-Step qRT-PCR Kit with ROX passive reference

For Research Use Only. Not for use in diagnostic procedures.
사양
용도(장비)7000 System, 7300 System, 7700 System, 7900HT System
반응 수500 Reactions
중합효소Platinum Taq DNA Polymerase
제품라인Platinum, SuperScript
제품 유형One-Step qRT-PCR Kit
수량500 reactions
샘플 종류RNA
배송 조건Dry Ice
충분500 Reactions
검출 방법Primer-probe
용도(애플리케이션)Gene Expression, Pathogen Detection, Virus Detection
PCR 방법1-step RT-qPCR
반응 속도Standard
Unit SizeEach
구성 및 보관
• 500 μL SuperScript™ III/Platinum™ Taq Mix
• 12.5 mL 2X Reaction Mix with ROX
• 2 × 1 mL Magnesium Sulfate (50 mM)

Store components in the dark at -20°C.

자주 묻는 질문(FAQ)

What can I do to improve the sensitivity of my qPCR assay?

If you are targeting a low-abundance gene, you may have trouble getting Ct values in a good, reliable range (Ct > 32). To increase the sensitivity of the assay, you may want to consider the following:

- Increase the amount of RNA input into your reverse transcription reaction, if possible
- Increase the amount of cDNA in your qPCR reaction (20% by volume max)
- Try a different reverse transcription kit, such as our SuperScript VILO Master Mix, for the highest cDNA yield possible
- Consider trying a one-step or Cells-to-CT type workflow (depending on your sample type)

How do I set the baseline for my qPCR experiment?

Most times your instrument software can automatically set a proper baseline for your data. Check out our short video, Understanding Baselines, for more information on how to set them (https://www.youtube.com/watch?feature=player_embedded&v=5BjFAJHW-bE).

How do I set the threshold for my qPCR experiment?

In most cases your instrument software can automatically set a proper threshold for your data. Check out our short video, Understanding Thresholds, for more information on how to set them (https://www.youtube.com/watch?feature=player_embedded&v=H_xsuRQIM9M).

I am not getting any amplification with my TaqMan Assay or SYBR Green primer set. What is causing this?

There could be several reasons for no amplification from an assay or primer set. Please see these examples and suggested solutions in our Real-Time Troubleshooting Tool (https://www.thermofisher.com/us/en/home/life-science/pcr/real-time-pcr/qpcr-education/real-time-pcr-troubleshooting-tool/gene-expression-quantitation-troubleshooting/no-amplification.html) for more details.

I am getting amplification in my no-template control (NTC) wells in my qPCR experiment. What is causing this?

There could be several reasons for amplification in a NTC well. Please see these examples and suggested solutions in our Real-Time Troubleshooting Tool (https://www.thermofisher.com/us/en/home/life-science/pcr/real-time-pcr/qpcr-education/real-time-pcr-troubleshooting-tool/gene-expression-quantitation-troubleshooting/amplification-no-template-control.html) for more details.

View all SuperScript™ III Platinum™ One-Step qRT-PCR Kit w/ROX, 500 reactions FAQs

인용 및 참조 문헌 (1)

인용 및 참조 문헌
Abstract
Analysis of one-step and two-step real-time RT-PCR using SuperScript III.
Authors:Wacker MJ,Godard MP
Journal:Journal of biomolecular techniques : JBT
PubMed ID:16461951
Real-time reverse transcription polymerase chain reaction (RT-PCR) is a commonly used technique to analyze gene expression. There has been little research conducted to test if SuperScript III quantitative one-step (reverse transcription carried out in the same tube as PCR) and two-step (reverse transcription carried out in a separate reaction) RT-PCR ... More