96-Well Plates for Pierce™ BCA-RAC Assay

The reconstituted compatibility reagent is stable for 8 hours when stored at 4 degrees C protected from light.

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The BCA Protein Assay Kit - Reducing Agent Compatible (Cat. No. 23250) has sufficient reagents for 250 samples or standards of 25µL each, and the Microplate BCA Protein Assay Kit - Reducing Agent Compatible (Cat. No. 23252) can be used to assay 1,000 samples or standards of 9µL each.

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No. Increasing the sample and compatibility reagent volumes will require increasing the BCA working reagent, resulting in a volume that exceeds the well capacity. There must be space remaining in the well to allow mixing and prevent the solution from contacting the sealing tape.

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There are two different formats: the BCA Protein Assay Kit - Reducing Agent Compatible (Cat. No. 23250) for microcentrifuge tube (1.5ml) assays and Microplate BCA Protein Assay Kit - Reducing Agent Compatible (Cat. No. 23252) for 96-well plates. The tube assay is compatible with 5mM DTT, 10mM TCEP and 35mM 2ME, and the microplate assay is compatible with 5mM DTT, 10mM TCEP and 25mM 2ME.

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The BCA-RAC provides the advantages of the original BCA assay (compatibility with detergents, buffer/salts and solvents) combined with reducing agent compatibility at concentrations routinely used in protein sample buffers. The BCA assay is based on reduction of Cu+2 to Cu+1 by proteins in an alkaline solution and the subsequent complexing of the Cu+1 to BCA reagent, yielding a purple color detectable at 562nm. Disulfide reducing agents such as DTT, 2ME and TCEP are also capable of reducing Cu+2 to Cu+1. To minimize their reducing effect, a compatibility reagent that modifies disulfide reducing agents is added to the sample before performing the assay.

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