Opti-MEM™ I Reduced Serum Medium
Opti-MEM™ I Reduced Serum Medium
인용 및 참조 문헌 (10)
Gibco™

Opti-MEM™ I Reduced Serum Medium

Opti-MEM™ I Reduced-Serum Medium은 개선된 Minimal Essential Medium(MEM)으로 소 태아 혈청(Fetal Bovine Serum) 첨가량을 성장 속도나 형태학적 변화 없이 적어도자세히 알아보기
Have Questions?
보기 방식 변경buttonViewtableView
카탈로그 번호수량
31985070500 mL
3198508810 x 500 mL
31985062100 mL
카탈로그 번호 31985070
제품 가격(KRW)
90,000
キャンペーン価格
Ends: 31-Dec-2025
94,000
할인액 4,000 (4%)
Each
카트에 추가하기
수량:
500 mL
제품 가격(KRW)
90,000
キャンペーン価格
Ends: 31-Dec-2025
94,000
할인액 4,000 (4%)
Each
카트에 추가하기
Opti-MEM™ I Reduced-Serum Medium은 개선된 Minimal Essential Medium(MEM)으로 소 태아 혈청(Fetal Bovine Serum) 첨가량을 성장 속도나 형태학적 변화 없이 적어도 50% 이상 절감할 수 있습니다. Opti-MEM™ I 배지는 또한 Lipofectamine™ 시약과 같은 양이온 지질 형질주입 시약과 함께 사용하는 것이 권장됩니다. Opti-MEM™ I 배지는 Sp2, AE-1, CHO, BHK-21, HEK, 일차 섬유아세포(primary fibroblast) 등 다양한 현탁 및 부착 포유류 세포에 사용할 수 있습니다. 당사는 다양한 조성의 Opti-MEM™ I 제품으로 광범위한 세포 배양 응용 분야를 지원합니다.

이 Opti-MEM™ I은 다음과 같이 조성이 변경됩니다.
포함:
• L-글루타민
• 페놀 레드(phenol red)

전체 조성은 비공개 사항입니다. 자세한 내용은 기술 서비스팀에문의하십시오.

Opti-MEM™ I 배지의 사용
Opti-MEM™ I Reduced-Serum Medium은 인슐린, 트랜스페린(transferrin), 하이포크산틴(hypoxanthine), 티미딘(thymidine) 및 미량 원소를 포함하는 고유한 배지입니다. 이러한 추가 성분으로 혈청 첨가량을 적어도 50% 만큼 줄일 수 있습니다. Opti-MEM™ I 배지는 중탄산나트륨(sodium bicarbonate) 버퍼 시스템(2.4 g/L)을 이용하며, 이로 인해 생리적학적 pH를 유지하기 위해 5–10% CO2 환경이 요구됩니다.

cGMP 제조 및 품질 시스템
Opti-MEM™ I는 영국 스코틀랜드의 Paisley에 위치한 cGMP 규정 준수 시설에서 제조됩니다. 이 시설은 의료기기 제조업체로 FDA에 등록되어 있으며 ISO 13485 스탠더드 인증을 받았습니다. 공급망 유지를 위해 당사는 또한 Grand Island 시설에서 동일한 Opti-MEM™ I 제품을 제조하여 공급합니다(31985-070). 이 시설도 또한 의료기기 제조업체로 FDA에 등록되어 있으며 ISO 13485 스탠더드 인증을 받았습니다.

This product is not used for in vitro diagnostic purpose in some countries.
For Research Use or Further Manufacturing. Not for diagnostic use or direct administration into humans or animals.
사양
세포주Sp2, AE-1, CHO, BHK-21, and HEK
세포 유형Primary Fibroblasts
농도1 X
제조 품질cGMP-compliant under the ISO 13485 standard
제품라인Gibco, Opti-MEM
제품 유형Opti-MEM I
수량500 mL
유통 기한18 Months From Date of Manufacture
배송 조건Room Temperature
분류Human Origin
형태Liquid
멸균Sterile-filtered
Sterilization MethodSterile-filtered
첨가제 포함Glutamine, Phenol Red
Unit SizeEach
구성 및 보관
Store at 2–8°C and protect from light.

자주 묻는 질문(FAQ)

What should I use to dilute my RNAi duplexes and Lipofectamine RNAiMAX before complexing?

We recommend using Opti-MEM I Reduced Serum Medium (Cat. No. 31985062).

Find additional tips, troubleshooting help, and resources within our Transfection Support Center.

Do you offer an alternative for Opti-MEM I Reduced Serum Medium (Cat. No. 31985070)?

Opti-MEM I Reduced Serum Medium, no phenol red (Cat. No. 11058021) would be the closest alternative. Both offerings contain L-glutamine. For more a stabilized L-glutamine option, Opti-MEM I Reduced Serum Medium, GlutaMAX Supplement (Cat. No. 51985034) is the recommended alternative. All Opti-MEM offerings have demonstrated performance for both reduced-serum cell culture and lipid-based transfection.

My OpitMEM Reduced Serum Medium (Cat. No. 31985070) was accidentally frozen. Can I still use it?

This product should be stored at 2-8 degrees C and should not be frozen. The biggest issue with accidentally freezing this product is its solubility. If this product was accidentally frozen, we recommend placing it in a 2-8 degree environment and allowing it to slowly thaw overnight. If the product is fully thawed and shows no signs of precipitation, then it should still be usable, but we cannot guarantee effectiveness.

Find additional tips, troubleshooting help, and resources within our Cell Culture Support Center.

Is there any other difference between Opti-MEM I Reduced Serum Medium and Opti-MEM I Reduced Serum Medium, no phenol red besides the presence/absence of phenol red?

The formulation of Opti-MEM I Reduced Serum Medium and that of Opti-MEM I Reduced Serum Medium, no phenol red is the same except for the presence of phenol red in the former medium.

Find additional tips, troubleshooting help, and resources within our Cell Culture Support Center.

Where can I find the osmolality for my lot of Opti-MEM I Reduced Serum Medium?

We do provide osmolality information on the certificate of analysis. All lots of Opti-MEM I Reduced Serum Medium will meet the osmolality specification of ≥280 to ≤320 mOsm/kg.

Find additional tips, troubleshooting help, and resources within our Cell Culture Support Center.

View all Opti-MEM™ I Reduced Serum Medium, 500 mL FAQs

인용 및 참조 문헌 (10)

인용 및 참조 문헌
Abstract
Identification of a novel redox-sensitive gene, Id3, which mediates angiotensin II-induced cell growth.
Authors:Mueller Cornelius; Baudler Stephanie; Welzel Hilke; Böhm Michael; Nickenig Georg;
Journal:Circulation
PubMed ID:12021231
BACKGROUND: Reactive oxygen species, such as superoxide (O(2)(-)), are involved in the abnormal growth of various cell types. Angiotensin II (Ang II) is one of the most potent inducers of oxidative stress in the vasculature. The molecular events involved in Ang II-induced proliferation of vascular smooth muscle cells (VSMCs) are ... More
Regulation of cortical dendrite development by Slit-Robo interactions.
Authors:Whitford Kristin L; Marillat Valérie; Stein Elke; Goodman Corey S; Tessier-Lavigne Marc; Chédotal Alain; Ghosh Anirvan;
Journal:Neuron
PubMed ID:11779471
Slit proteins have previously been shown to regulate axon guidance, branching, and neural migration. Here we report that, in addition to acting as a chemorepellant for cortical axons, Slit1 regulates dendritic development. Slit1 is expressed in the developing cortex, and exposure to Slit1 leads to increased dendritic growth and branching. ... More
Non-viral-mediated gene transfer of OX40 ligand for tumor immunotherapy.
Authors:Rakitina OA,Kuzmich AI,Bezborodova OA,Kondratieva SA,Pleshkan VV,Zinovyeva MV,Didych DA,Sass AV,Snezhkov EV,Kostina MB,Koksharov MO,Alekseenko IV
Journal:Frontiers in immunology
PubMed ID:39007148
BACKGROUND: Immune checkpoint blockade (ICB) is rapidly becoming a standard of care in the treatment of many cancer types. However, the subset of patients who respond to this type of therapy is limited. Another way to promote antitumoral immunity is the use of immunostimulatory molecules, such as cytokines or T ... More
Effects of an indole derivative on cell proliferation, transfection, and alternative splicing in production of lentiviral vectors by transient co-transfection.
Authors:Mier NC,Roper DK
Journal:PloS one
PubMed ID:38833479
Lentiviral vectors derived from human immunodeficiency virus type I are widely used to deliver functional gene copies to mammalian cells for research and gene therapies. Post-transcriptional splicing of lentiviral vector transgene in transduced host and transfected producer cells presents barriers to widespread application of lentiviral vector-based therapies. The present study ... More
Aldose reductase mediates mitogenic signaling in vascular smooth muscle cells.
Authors: Ramana Kota V; Chandra Deepak; Srivastava Sanjay; Bhatnagar Aruni; Aggarwal Bharat B; Srivastava Satish K;
Journal:J Biol Chem
PubMed ID:12063254
'Abnormal vascular smooth muscle cell (VSMC) proliferation is a key feature of atherosclerosis and restenosis; however, the mechanisms regulating growth remain unclear. Herein we show that inhibition of the aldehyde-metabolizing enzyme aldose reductase (AR) inhibits NF-kappa B activation during restenosis of balloon-injured rat carotid arteries as well as VSMC proliferation ... More
View all Opti-MEM™ I Reduced Serum Medium, 500 mL citations