TaqMan™ TAMRA Probe
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TaqMan™ TAMRA Probe
Applied Biosystems™

TaqMan™ TAMRA Probe

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Applied Biosystems TaqMan TAMRA probes are dual-labeled probes used for real-time PCR applications using TaqMan chemistry. TaqMan TAMRA Probes feature자세히 알아보기
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카탈로그 번호수량제품 유형
4500256,000 pmolTAMRA Probe
45002420,000 pmolTAMRA Probe
45000350,000 pmolTAMRA Probe
카탈로그 번호 450025
제품 가격(KRW)
520,000
Online offer
Ends: 31-Dec-2025
577,000
할인액 57,000 (10%)
Each
주문하기
수량:
6,000 pmol
제품 유형:
TAMRA Probe
Applied Biosystems TaqMan TAMRA probes are dual-labeled probes used for real-time PCR applications using TaqMan chemistry. TaqMan TAMRA Probes feature a 5’ fluorescent reporter dye (FAM, VIC, or TET) and 3’ fluorescent quencher (TAMRA dye). TaqMan TAMRA probes, which were some of the first TaqMan probes to be developed, can be used for a variety of applications.

TAMRA is a fluorescent dye and can be multiplexed with up to three dyes. (If multiplexing higher than triplex, TaqMan QSY probes are recommended.) TAMRA probes are typically longer (30 to >40 bases). For enhanced specificity, shorter TaqMan MGB nonfluorescent quencher (NFQ) probes (generally 15 to 20 bases) are also available.

All TaqMan TAMRA probes are HPLC-purified and verified by mass spectrometry to help ensure lot-to-lot reproducibility between batches. Thermo Fisher Scientific has been a leader in qPCR probe manufacturing for over 20 years. TaqMan Probes are manufactured in a facility that is certified to ISO 13485.

Guaranteed yield
The unit size you select is based on the minimum amount we will deliver, not on synthesis starting amount.

Approximate ship time
4–6 days in North America, 5-10 days in Europe

We also offer Applied Biosystems custom primers and other types of TaqMan probes. Learn more ›

Need a different dye or concentration or a larger lot size? The TaqMan Custom Assay and Oligo Service offers custom products that you can tailor to meet your experimental needs. Learn more ›
For Research Use Only. Not for use in diagnostic procedures.
사양
3'프라이머 수정TAMRA
5'프라이머 수정FAM, TET, VIC
용도(장비)7500 System, 7500 Fast System, 7900HT System, StepOne™, StepOnePlus™, ViiA™ 7 System, QuantStudio™ 3, QuantStudio™ 5, QuantStudio™ 6 Flex, QuantStudio™ 7 Flex, QuantStudio 6 Pro, QuantStudio 7 Pro, QuantStudio™ 12k Flex
형식Tube
그린 기능Sustainable packaging
내부 프로브 수정None
제품라인TaqMan
제품 유형TAMRA Probe
정제 방법HPLC
순도 또는 품질 등급HPLC
수량6,000 pmol
배송 조건Room Temperature
용도(애플리케이션)Real Time PCR (qPCR)
형태Liquid or Dried Down
Unit SizeEach
구성 및 보관
1 tube containing a dual-labeled probe in solution or dried down.

Store at -15°C to -25°C

자주 묻는 질문(FAQ)

I would like to calibrate my Real-Time PCR instrument with a custom dye, which buffer should I use?

Please ask your dye manufacturer for recommendations. If there is no specific recommendation available, try TE Buffer (pH 8.0) to start.

I need to see all the primer and probe sequences from an assay design right away. How can I do this?

You can directly see all the primer and probe sequences from a test design right away using the Primer Express Software. Open the software and choose File => New. Choose either TaqMan MGB Quantification or TaqMan Quantification for Gene Expression Assay design. Copy/paste your gene sequence of interest into the new window and click on the green triangle to get primer/probe designs.

Find additional tips, troubleshooting help, and resources within our Real-Time PCR and Digital PCR Instruments Support Center.

How can I determine whether the Tm of my custom probe is appropriate?

In general, the target Tm of a TaqMan probe is 70 degrees C. Using the Primer Express Software, you can check the Tm of a MGB or TAMRA probe probe.

Within the software, go to Primer Probe Test Tool and then copy/paste in your sequence of interest to see the Tm. If you need to check for a MGB based probe, make sure to choose Document Type: TaqMan MGB Quantification. If you need to check for a QSY or TAMRA based probe, make sure to choose Document Type: TaqMan Quantification. Real-Time PCR and Digital PCR Applications

Find additional tips, troubleshooting help, and resources within our Real-Time PCR and Digital PCR Applications Support Center.

I have purchased custom oligos (primers and probes) and I want to make a 20x working stock for a TaqMan assay, similar to the pre-designed assays. How do I dilute and mix my oligos to make a 20x working stock?

Most pre-designed TaqMan assays are supplied as a 20x assay concentrate containing 18 uM of each primer and 5 uM of probe which provides a 900 nM of each primer and 250 nM of the probe (for gene expression assays) and 200 nM of each probe (for SNP genotyping assays) at the final 1x concentration.

In order to make a 20x working stock from custom oligos, the oligos need to be diluted as follows: To make a 20x Assay stock for Gene Expression assays, make 100 uM stocks of both forward and reverse primers, and TaqMan probe. Add 18 ul of each primer and 5 ul of the TaqMan probe, which gives 41 ul volume. Add 59 ul of 1X TE to bring the volume to 100 ul of 20X TaqMan Gene Expression Assay. To make a 20x Assay stock for a Genotyping Assay, make 100 uM stocks of both forward and reverse primers, and both TaqMan probes. Add 18 ul of each primer and 4 ul of each TaqMan probe, which gives 44 ul volume. Add 56 ul of 1X TE to bring the volume to 100 ul of 20X TaqMan SNP Genotyping Assays.

I am looking to order a molecular probe for validation, using specific sequences. How do I order a custom probe?

We do not offer any custom probes for diagnostic purposes.

We do offer custom TaqMan probes for qPCR experiments, which are for Research Use Only.
TaqMan MGB Probe
TaqMan QSY Probe
TaqMan TAMRA Probe
These above probes can be designed in our online tool, Custom TaqMan Probes.

If you would like to order a pre-mix of primers and probe, please check our custom TaqMan Assays.
You can find more information one the Custom TaqMan Gene Expression Assay page on thermofisher.com.

Find additional tips, troubleshooting help, and resources within our Real-Time PCR and Digital PCR Applications Support Center.

View all TaqMan™ TAMRA Probe, 6,000 pmol FAQs