Neurobasal™-A Medium, no D-glucose, no sodium pyruvate
Neurobasal™-A Medium, no D-glucose, no sodium pyruvate
Gibco™

Neurobasal™-A Medium, no D-glucose, no sodium pyruvate

Neurobasal-A medium without D-glucose and sodium pyruvate is used to support the growth of post-natal CNS neurons in applications where자세히 알아보기
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카탈로그 번호수량
A2477501500 mL
카탈로그 번호 A2477501
제품 가격(KRW)
142,000
Online offer
Ends: 31-Dec-2025
157,000
할인액 15,000 (10%)
Each
카트에 추가하기
수량:
500 mL
제품 가격(KRW)
142,000
Online offer
Ends: 31-Dec-2025
157,000
할인액 15,000 (10%)
Each
카트에 추가하기
Neurobasal-A medium without D-glucose and sodium pyruvate is used to support the growth of post-natal CNS neurons in applications where the presence of D-glucose and sodium pyruvate can cause interference.

For neural studies that require the absence of insulin, glucose, and sodium pyruvate, such as glucose response or insulin response studies, we recommend Gibco B-27 Supplement Minus Insulin as a supplement to this medium.

Neurobasal Medium is suitable for use in most neuronal cell applications. For other applications, we provide alternative formulations of Neurobasal media and B-27 supplements.

For established protocols using Neurobasal media, access our Gibco Neurobiology Protocol Handbook.

View publications using Gibco neurobiology products at our Gibco CellCite.

For Research Use Only. Not for use in diagnostic procedures.
사양
세포 유형Neural Cells
제품라인Neurobasal
제품 유형Neurobasal Medium
수량500 mL
유통 기한12 Months From Date of Manufacture
배송 조건Ambient
테스트됨Endotoxin, Performance, Sterility, Osmolality, pH
분류Serum-free
Culture TypeNeuronal Cell Culture
형태Liquid
첨가제 포함Phenol Red
첨가제 없음No Glucose, No Sodium Pyruvate
Unit SizeEach
구성 및 보관
Store in refrigerator (2–8°C) and protect from light.

자주 묻는 질문(FAQ)

Can you provide references for growing adult neurons?

Instructions for growing adult neurons can be found in the Neurobasal-A Medium product insert. You can also refer to the following publication for growing adult neurons:
• Isolation and Culture of Adult Rat Hippocampal Neurons by Brewer, G.J., J. Neuroscience Methods 71 (1997), 145-158.

Should I use Neurobasal-A Medium with B27 and b-FGF for postnatal neurons?

We do not know at what age postnatal neurons require FGF and a higher osmolality medium, or how this could vary based on the species from which the neural tissue is isolated. Without this knowledge, we generally recommend switching to the Neurobasal-A Medium for isolates from animals above 1 week of age. You will need to determine if the Neurobasal-A Medium is optimal for neurons you have from one day old rats.

Further, the addition of bFGF can increase the viability of neurons of any age. Therefore, caution needs to be made in impure neuronal cell culture as bFGF can increase the proliferation of non-neuronal cells. We recommend a pilot experiment to test the impure culture in the presence and absence of bFGF.

Find additional tips, troubleshooting help, and resources within our Cell Culture Support Center.

What should I add to Neurobasal-A Medium to grow adult neurons?

We recommend adding B27 and b-FGF to Neurobasal-A Medium to grow adult neurons.

Find additional tips, troubleshooting help, and resources within our Cell Culture Support Center.

What did you add to Neurobasal-A Medium to increase osmolality?

4 g/L of NaCl has been added to the Neurobasal-A Medium formulation to increase osmolality.

Find additional tips, troubleshooting help, and resources within our Cell Culture Support Center.