SuperSignal™ Western Blot Substrate Bundle, Femto + trial-size Atto
SuperSignal™ Western Blot Substrate Bundle, Femto + trial-size Atto
Thermo Scientific™

SuperSignal™ Western Blot Substrate Bundle, Femto + trial-size Atto

This substrate bundle contains SuperSignal West Femto Maximum Sensitivity Chemiluminescent Substrate plus a sample size of SuperSignal West Atto Ultimate자세히 알아보기
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카탈로그 번호수량
A45916100 mL + 20 mL bundle
카탈로그 번호 A45916
제품 가격(KRW)
792,000
Each
카트에 추가하기
수량:
100 mL + 20 mL bundle
제품 가격(KRW)
792,000
Each
카트에 추가하기
This substrate bundle contains SuperSignal West Femto Maximum Sensitivity Chemiluminescent Substrate plus a sample size of SuperSignal West Atto Ultimate Sensitivity Chemiluminescent Substrate to enable trial and comparison of the newest and most sensitive substrate for low abundant targets.

SuperSignal West Femto Maximum Sensitivity Substrate
Thermo Scientific SuperSignal West Femto Maximum Sensitivity Substrate is a high-sensitivity enhanced chemiluminescent (ECL) substrate for low femtogram protein level detection by western blot analysis with horseradish peroxidase (HRP) enzyme. It is optimized to detect low abundant and/or difficult-to-detect targets.

• Sensitivity: low-femtogram (mid-zeptomole)
• Stability: eight hours for working solution, one year for kit at 4°C
• Compatibility: nitrocellulose and PVDF membranes
• Signal duration: up to eight hours
• Recommended primary antibody concentration: 1:5,000–1:100,000 dilution (10–200 ng/mL)
• Recommended secondary antibody concentration: 1:100,000–1:500,000 dilution (2–10 ng/mL)

View full product information ›

SuperSignal West Atto Ultimate Sensitivity Chemiluminescent Substrate
Thermo Scientific SuperSignal West Atto Ultimate Sensitivity Chemiluminescent Substrate is an ultra-sensitive enhanced chemiluminescent (ECL) substrate for high attogram protein level detection by western blot analysis with horseradish peroxidase (HRP) enzyme. It is designed for detection of very low-abundant targets or the maximum levels of sensitivity required when using precious samples or costly antibodies.

• Sensitivity: low-femtogram to high-attogram
• Stability: eight hours for working solution, one year for kit at 4°C
• Compatibility: nitrocellulose and PVDF membranes
• Signal duration: up to four hours
• Recommended primary antibody concentration: 1:1,000–1:5,000 dilution (0.2–1.0 μg/mL)
• Recommended secondary antibody concentration: 1:100,000–1:250,000 dilution (4–10 ng/mL)

View full product information ›

For Research Use Only. Not for use in diagnostic procedures.
사양
민감도Low-femtogram (mid-zeptomole)
배송 조건Approved for shipment at Room Temperature or on Wet or Dry Ice
충분12 Mini-Gel Blots, 1000 sq. cm of Membrane; 2 Mini-Gel Blots, 200 sq. cm of Membrane
검출 방법Chemiluminescence
용도(애플리케이션)Western Blot
형태Liquid
Membrane CompatibilityNitrocellulose, PVDF
제품라인SuperSignal
제품 유형Substrate
수량100 mL + 20 mL bundle
SubstrateHorseradish Peroxidase
Unit SizeEach
구성 및 보관
• 100 mL SuperSignal West Femto reagent, store at 2–8°C
• 20 mL SuperSignal West Atto reagent, store at 2–8°C

자주 묻는 질문(FAQ)

How do I handle saturated or burned out bands using SuperSignal West Atto Ultimate Sensitivity Substrate?

Here are some suggestions:
- Shorten the time of exposure
- Wash blot and try using SuperSignal West Pico PLUS Chemiluminescent Substrate
- Strip the membrane using Restore Western Blot Stripping Buffer, re-probe with lesser amount of primary and secondary antibodies, and try first with SuperSignal West Pico PLUS Chemiluminescent Substrate
- Re-run the gel using lesser sample

Find additional tips, troubleshooting help, and resources within our Protein Assays and Analysis Support Center.

How can I reduce background with SuperSignal West Atto Ultimate Sensitivity Substrate?

Here are potential causes for high background and corresponding solutions:
- Too much HRP in the system: Further dilute the HRP-conjugate, see guidelines in Table 1 in the user guide (https://assets.thermofisher.com/TFS-Assets/LSG/manuals/MAN0018858_SuperSignalWestAtto_UG.pdf).
- Inadequate blocking: Optimize blocking conditions.
- Inappropriate blocking reagent: Try a different blocking reagent. Use clear milk or StartingBlock blocking buffer instead of dry milk.
- Inadequate washing: Increase length, number or volume of washes.
- Film has been overexposed: Decrease exposure time or use Pierce Background Eliminator (Cat No. 21065).
- Concentration of antigen or antibody is too high: Decrease amount of antigen or antibody.
- Poor antibody specificity: Use SuperSignal Western Blot Enhancer (Cat. No. 46640).
We also recommend minimizing handling of the membrane, using clean gloves while handling, clean plasticware, glassware, forceps, or other contact surfaces. We also recommend trimming of the membrane to remove excess area.

Find additional tips, troubleshooting help, and resources within our Protein Assays and Analysis Support Center.

Is SuperSignal West Atto Ultimate Sensitivity Substrate compatible with Restore Western Blot Stripping Buffer?

SuperSignal West Atto Ultimate Sensitivity Substrate is compatible with Restore Western Blot Stripping Buffer.

Find additional tips, troubleshooting help, and resources within our Protein Assays and Analysis Support Center.

When should I use SuperSignal West Atto Ultimate Sensitivity Substrate?

We recommend using SuperSignal West Atto Ultimate Sensitivity Substrate for detection of proteins in the following situations:
- For proteins with low concentration, not measurable by BCA or Bradford assays
- For low-abundance phosphorylated or glycosylated proteins
- For low-abundance membrane or nuclear proteins
- For low-abundance proteins being detected with a low-affinity antibody or when the antibody is precious
- When sample source is limited or when multiple conditions or assays are being tested on a single sample

Find additional tips, troubleshooting help, and resources within our Protein Assays and Analysis Support Center.

Is SuperSignal West Atto Ultimate Sensitivity Substrate affected by freeze/thaw cycles?

SuperSignal West Atto Ultimate Sensitivity Substrate can tolerate up to 3 freeze/thaw cycles without significant loss in performance.

Find additional tips, troubleshooting help, and resources within our Protein Assays and Analysis Support Center.

View all SuperSignal™ Western Blot Substrate Bundle, Femto + trial-size Atto FAQs