The VetMAX M. tuberculosis Complex PCR Kit is a single-well, duplex, real-time PCR assay for the detection of all mycobacteria belonging to the Mycobacterium tuberculosis Complex in bovine lymph nodes and surrounding tissues. The kit is a highly sensitive and specific test for the detection of bovine tuberculosis, allowing reliable and rapid screening, exclusion of non-infected animals, and confirmation of infected animals.
All components needed for the PCR reaction are included in the kit: • Mix MTBC: contains TaqMan primers and probes, buffer, and enzyme for optimized duplex real-time PCR amplification of targets • EPC MTBC: DNA template containing the M. tuberculosis Complex target sequence (external positive control). It serves as a positive control for the real-time PCR components and is used to set the threshold cycle (Ct) for evaluating test results • IPC MTBC: added to each test and control sample at the lysis step of the DNA isolation procedure (exogenous internal positive control). The IPC serves as a control for the DNA isolation procedure and is used to monitor for the presence of PCR inhibitors
The VetMAX M. tuberculosis Complex PCR Kit is part of a workflow solution with the MagVet Universal Isolation Kit (Cat. No. MV384) and the Kingfisher Flex Magnetic Particle Processor or Kingfisher mL Flex Magnetic Particle Processor (Cat. Nos. 5400630 and 5400640).