Gateway™ pDEST™10 Vector

Citations & References (6)

Invitrogen™

Gateway™ pDEST™10 Vector

To fit all of your expression needs, Invitrogen offers state-of-the-art Gateway™ destination vectors for expression in E. coli, insect, yeast,Read more

Catalog Number	Quantity
11806015	6 μg

Catalog number 11806015

Price (MXN)

Quantity:

To fit all of your expression needs, Invitrogen offers state-of-the-art Gateway™ destination vectors for expression in E. coli, insect, yeast, or mammalian cells, as well as for production of native protein or N- or C-terminal fusion proteins. All Gateway™ destination vectors have attR sites for recombination with any attL-flanked fragment, regardless of whether it is an entry clone or an Ultimate™ RF Clone. The following table lists the wide range of destination vectors available.

Additional materials required, available separately: Gateway™ entry clone, Gateway™ LR Clonase™ enzyme mix, and reaction buffer.

For Research Use Only. Not for use in diagnostic procedures.

Specifications

CleavageTEV Protease Recognition Site

Product TypeGateway System Destination Expression Vector

Quantity6 μg

VectorpDEST, Gateway Baculovirus Vectors

Cloning MethodGateway

Product LineGateway

PromoterPolyhedrin

Protein TagHis Tag (6x)

Unit SizeEach

Contents & Storage

All destination vectors are provided lyophilized and supercoiled.

Frequently asked questions (FAQs)

Can I perform the single-step protocol for the BP/LR Clonase reaction using BP Clonase enzyme and LR Clonase enzyme instead of BP Clonase II enzyme and LR Clonase II enzyme?

In the single-step protocol for the BP/LR Clonase reaction, we would not recommend substituting the BP Clonase II/LR Clonase II enzymes with BP Clonase /LR Clonase enzymes as this would result in very low recombination efficiency.

Do you have a recommended single-step protocol for BP/LR recombination?

Yes, we have come up with a single-step protocol for BP/LR Clonase reaction (http://www.thermofisher.com/us/en/home/life-science/cloning/gateway-cloning.html#1), where DNA fragments can be cloned into Destination vectors in a single step reaction, allowing you to save time and money.

How can I move my gene of interest from a Gateway-adapted expression clone to a new Destination vector as I have lost the entry clone?

We would recommend performing a BP reaction with a Donor vector in order to obtain an entry clone. This entry clone can then be used in an LR reaction with the Destination vector to obtain the new expression clone.

Can I purchase the 5X LR Clonase buffer or 5X BP Clonase buffer separately?

We do not offer the 5X LR Clonase buffer and 5X BP Clonase buffer as standalone products. They are available as part of the enzyme kits.

Do you offer Gateway vectors for expression in plants?

We do not offer any Gateway vectors for expression in plants.

View all Gateway™ pDEST™10 Vector FAQs

Citations & References (6)

Citations & References

Abstract

Munc13-4 is a GTP-Rab27-binding protein regulating dense core granule secretion in platelets.

Authors:Shirakawa R, Higashi T, Tabuchi A, Yoshioka A, Nishioka H, Fukuda M, Kita T, Horiuchi H,

Journal:J Biol Chem

PubMed ID:14699162

'Platelets store self-agonists such as ADP and serotonin in dense core granules. Although exocytosis of these granules is crucial for hemostasis and thrombosis, the underlying mechanism is not fully understood. Here, we show that incubation of permeabilized platelets with unprenylated active mutant Rab27A-Q78L, wild type Rab27A, and Rab27B inhibited the ... More

SCAP ligands are potent new lipid-lowering drugs.

Authors: Grand-Perret T; Bouillot A; Perrot A; Commans S; Walker M; Issandou M;

Journal:Nat Med

PubMed ID:11726962

'Upregulation of low-density lipoprotein receptor (LDLr) is a key mechanism to control elevated plasma LDL-cholesterol levels. Here we identify a new class of compounds that directly binds to the sterol regulatory element-binding protein (SREBP) cleavage-activating protein (SCAP). We show that a 14C-labeled, photo-activatable analog specifically labeled both SCAP and a ... More

Enhanced production of green fluorescent fusion proteins in a baculovirus expression system by addition of secretion signal.

Authors:Katagiri Y, Ingham KC.

Journal:Biotechniques

PubMed ID:12139250

DNA cloning using in vitro site-specific recombination.

Authors: Hartley J L; Temple G F; Brasch M A;

Journal:Genome Res

PubMed ID:11076863

As a result of numerous genome sequencing projects, large numbers of candidate open reading frames are being identified, many of which have no known function. Analysis of these genes typically involves the transfer of DNA segments into a variety of vector backgrounds for protein expression and functional analysis. We describe ... More

Role of the G protein gamma subunit in beta gamma complex modulation of phospholipase Cbeta function.

Authors: Akgoz Muslum; Azpiazu Inaki; Kalyanaraman Vani; Gautam N;

Journal:J Biol Chem

PubMed ID:11914377

The G protein betagamma complex regulates a wide range of effectors, including the phospholipase C isozymes (PLCbetas). Different domains on the beta subunit are known to contact phospholipase Cbeta and affect its regulation. In contrast, the role of the gamma subunit in Gbetagamma modulation of PLCbeta function is not known. ... More

View all Gateway™ pDEST™10 Vector citations