Munc13-4 is a GTP-Rab27-binding protein regulating dense core granule secretion in platelets.
AuthorsShirakawa R, Higashi T, Tabuchi A, Yoshioka A, Nishioka H, Fukuda M, Kita T, Horiuchi H,
JournalJ Biol Chem
PubMed ID14699162
'Platelets store self-agonists such as ADP and serotonin in dense core granules. Although exocytosis of these granules is crucial for hemostasis and thrombosis, the underlying mechanism is not fully understood. Here, we show that incubation of permeabilized platelets with unprenylated active mutant Rab27A-Q78L, wild type Rab27A, and Rab27B inhibited the ... More
SCAP ligands are potent new lipid-lowering drugs.
Authors Grand-Perret T; Bouillot A; Perrot A; Commans S; Walker M; Issandou M;
JournalNat Med
PubMed ID11726962
'Upregulation of low-density lipoprotein receptor (LDLr) is a key mechanism to control elevated plasma LDL-cholesterol levels. Here we identify a new class of compounds that directly binds to the sterol regulatory element-binding protein (SREBP) cleavage-activating protein (SCAP). We show that a 14C-labeled, photo-activatable analog specifically labeled both SCAP and a ... More
Enhanced production of green fluorescent fusion proteins in a baculovirus expression system by addition of secretion signal.
AuthorsKatagiri Y, Ingham KC.
JournalBiotechniques
PubMed ID12139250
DNA cloning using in vitro site-specific recombination.
Authors Hartley J L; Temple G F; Brasch M A;
JournalGenome Res
PubMed ID11076863
As a result of numerous genome sequencing projects, large numbers of candidate open reading frames are being identified, many of which have no known function. Analysis of these genes typically involves the transfer of DNA segments into a variety of vector backgrounds for protein expression and functional analysis. We describe ... More
Role of the G protein gamma subunit in beta gamma complex modulation of phospholipase Cbeta function.
The G protein betagamma complex regulates a wide range of effectors, including the phospholipase C isozymes (PLCbetas). Different domains on the beta subunit are known to contact phospholipase Cbeta and affect its regulation. In contrast, the role of the gamma subunit in Gbetagamma modulation of PLCbeta function is not known. ... More
p53 recruitment of CREB binding protein mediated through phosphorylated CREB: a novel pathway of tumor suppressor regulation.
Authors Giebler H A; Lemasson I; Nyborg J K;
JournalMol Cell Biol
PubMed ID10848610
CREB binding protein (CBP) is a 270-kDa nuclear protein required for activated transcription of a large number of cellular genes. Although CBP was originally discovered through its interaction with phosphorylated CREB (pCREB), it is utilized by a multitude of cellular transcription factors and viral oncoproteins. Both CREB and the tumor ... More