Penicillin-Streptomycin (10,000 U/mL)

Citations & References (34)

Gibco™

Penicillin-Streptomycin (10,000 U/mL)

Name: Penicillin-Streptomycin (10,000 U/mL)
SKU: 15140122
Price: 604.04 MXN

This solution contains 10,000 units/mL of penicillin and 10,000 μg/mL of streptomycin. The antibiotics penicillin and streptomycin are used toRead more

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Catalog Number	Quantity
15140122 also known as 15140-122	100 mL
15140148 also known as 15140-148	20 mL
15140163 also known as 15140-163	20 x 100 mL

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Catalog number 15140122

also known as 15140-122

Price (MXN)

604.04

Each

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Quantity:

100 mL

Price (MXN)

604.04

Each

Add to cart

This solution contains 10,000 units/mL of penicillin and 10,000 μg/mL of streptomycin. The antibiotics penicillin and streptomycin are used to prevent bacterial contamination of cell cultures due to their effective combined action against gram-positive and gram-negative bacteria. Penicillin was originally purified from the fungi Penicillium and acts by interfering directly with the turnover of the bacterial cell wall and indirectly by triggering the release of enzymes that further alter the cell wall. Streptomycin was originally purified from Streptomyces griseus. It acts by binding to the 30S subunit of the bacterial ribosome, leading to inhibition of protein synthesis and death in susceptible bacteria.

We offer a wide range of antibiotics and antimycotics in both powder and liquid formats. Learn more about the following types of products:

• Cell Culture Antibiotics
• Selection Antibiotics (including recommended working concentrations)

Learn more about the use of antibiotics and antimycotics in cell culture and review guidelines for decontaminating cultures.

For Research Use Only. Not for use in diagnostic procedures.

Specifications

Concentration100 X

For Use With (Application)Prevention of Cell Culture Contamination

Quantity100 mL

Shelf Life12 Months

Shipping ConditionDry Ice

FormLiquid

Product TypeAntibiotic

SterilitySterile-filtered

Sterilization MethodSterile-filtered

Unit SizeEach

Contents & Storage

Storage conditions: -5°C to -20°C
Shipping conditions: Dry ice
Shelf life: 12 months from date of manufacture

Frequently asked questions (FAQs)

My Penicillin-Streptomycin solution is not colorless. Is this normal?

Yes, this is normal and will not affect the potency or application of the product. This solution is typically colorless. However, it can have a pink to yellow color tint. The coloring is a carry-over from the manufacturing process of Streptomycin - the genus that Steptomycin is isolated from (Actinomycetes Streptomyces griseus) is responsible for a wide variety of pigments.

Find additional tips, troubleshooting help, and resources within our Cell Culture Support Center.

How can I decontaminate my cultures?

When an irreplaceable culture becomes contaminated, researchers may attempt to eliminate or control the contamination.

1. Determine if the contamination is bacteria, fungus, mycoplasma, or yeast. Read more here to view characteristics of each contaminant.
2. Isolate the contaminated culture from other cell lines.
3. Clean incubators and laminar flow hoods with a laboratory disinfectant, and check HEPA filters.
4. Antibiotics and antimycotics at high concentrations can be toxic to some cell lines. Therefore, perform a dose-response test to determine the level at which an antibiotic or antimycotic becomes toxic. This is particularly important when using an antimycotic such as Gibco Fungizone reagent or an antibiotic such as tylosin.

The following is a suggested procedure for determining toxicity levels and decontaminating cultures:

1. Dissociate, count, and dilute the cells in antibiotic-free media. Dilute the cells to the concentration used for regular cell passage.
2. Dispense the cell suspension into a multiwell culture plate or several small flasks. Add the antibiotic of choice to each well in a range of concentrations. For example, we suggest the following concentrations for Gibco Fungizone reagent: 0.25, 0.50, 1.0, 2.0, 4.0, and 8.0 µg/mL.
3. Observe the cells daily for signs of toxicity such as sloughing, appearance of vacuoles, decrease in confluency, and rounding.
4. When the toxic antibiotic level has been determined, culture the cells for two to three passages using the antibiotic at a concentration one- to two-fold lower than the toxic concentration.
5. Culture the cells for one passage in antibiotic-free media.
6. Repeat step 4.
7. Culture the cells in antibiotic-free medium for four to six passages to determine if the contamination has been eliminated.

Find additional tips, troubleshooting help, and resources within our Cell Culture Support Center.

What antibiotics do you offer to help control or eliminate cell culture contamination?

Please view the following page to browse the cell culture antibiotics we offer (https://www.thermofisher.com/us/en/home/life-science/cell-culture/mammalian-cell-culture/antibiotics.html).

Find additional tips, troubleshooting help, and resources within our Cell Culture Support Center.

Citations & References (34)

Citations & References

Abstract

Identification of a novel redox-sensitive gene, Id3, which mediates angiotensin II-induced cell growth.

Authors:Mueller Cornelius; Baudler Stephanie; Welzel Hilke; BÃƒÂ¶hm Michael; Nickenig Georg;

Journal:Circulation

PubMed ID:12021231

BACKGROUND: Reactive oxygen species, such as superoxide (O(2)(-)), are involved in the abnormal growth of various cell types. Angiotensin II (Ang II) is one of the most potent inducers of oxidative stress in the vasculature. The molecular events involved in Ang II-induced proliferation of vascular smooth muscle cells (VSMCs) are ... More

Involvement of c-Src Tyrosine Kinase Upstream of Class I Phosphatidylinositol (PI) 3-Kinases in Salmonella Enteritidis Rck Protein-mediated Invasion.

Authors:Wiedemann A, Rosselin M, Mijouin L, Bottreau E, Velge P,

Journal:J Biol Chem

PubMed ID:22810232

'The Salmonella outer membrane protein Rck mediates a Zipper entry mechanism controlled by tyrosine phosphorylation and class I phosphatidylinositol 3-kinase (PI 3-kinase). However, the underlying mechanism leading to this signaling cascade remains unclear. The present study showed that using Rck-coated beads or Rck-overexpressing Escherichia coli, Rck-mediated actin polymerization and invasion ... More

Structural and energetic characteristics of the heparin-binding site in antithrombotic protein C.

Authors:Friedrich U, Blom AM, DahlbÃ¤ck B, Villoutreix BO,

Journal:J Biol Chem

PubMed ID:11316800

'Human activated protein C (APC) is a key component of a natural anticoagulant system that regulates blood coagulation. In vivo, the catalytic activity of APC is regulated by two serpins, alpha1-antitrypsin and the protein C inhibitor (PCI), the inhibition by the latter being stimulated by heparin. We have identified a ... More

Hypoxia induces the activation of the phosphatidylinositol 3-kinase/Akt cell survival pathway in PC12 cells: protective role in apoptosis.

Journal:J Biol Chem

PubMed ID:11294857

'Hypoxia is a common environmental stress that influences signaling pathways and cell function. Several cell types, including neuroendocrine chromaffin cells, have evolved to sense oxygen levels and initiate specific adaptive responses to hypoxia. Here we report that under hypoxic conditions, rat pheochromocytoma PC12 cells are resistant to apoptosis induced by ... More

Electrophoretic profiling of both RNA and protein from a single 250-pL sample.

Authors: Zabzdyr Jennifer L; Lillard Sheri J;

Journal:Anal Chem

PubMed ID:11985318

'A novel approach is described that uses capillary electrophoresis (CE) to electrophoretically sample and separate both protein and RNA from a single injected plug of cell lysate. A 250-pL sample of lysate from Chinese hamster ovary cells (9.6 x 10(7) cells/mL) was hydrodynamically injected into a capillary containing a Tris-based ... More

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