Product Image
Applied Biosystems™

GeneChip™ WT PLUS Reagent Kit

Gene transcripts profiling using GeneChip WT arrays is a well-proven technique for expression research and biomarker discovery. GeneChip WT PLUSRead more
Have Questions?
Change viewbuttonViewtableView
Catalog NumberQuantity
90228130 Reactions
90228010 Reactions
Catalog number 902281
Price (MXN)
-
Quantity:
30 Reactions
Gene transcripts profiling using GeneChip WT arrays is a well-proven technique for expression research and biomarker discovery. GeneChip WT PLUS Reagent Kit (WT PLUS Kit), with its high sensitivity and specificity, offers a robust WT assay to unravel the transcription signatures in your samples at both gene and exon levels.

WT PLUS Kit uses a reverse transcription priming method that primes the entire length of each RNA transcript, including both poly-A and non-poly-A mRNA to provide complete and unbiased coverage of the transcriptome. The kit efficiently generates amplified and biotinylated sense-stranded DNA targets, avoiding loss of specificity due to antisense strand interference.

The kit includes the amplification module coupled with the GeneChip WT Terminal Labeling and Controls Kit for preparing hybridization-ready targets from 50 ng to 500 ng of total RNA.

Features and benefits:
• A complete target prep kit with proven sensitivity and specificity resulting from an optimized design for WT arrays
• A low sample input requirement of 50 ng. The kit works with a variety of sample types, including fresh-frozen tissues, cell lines, and whole blood
• StreamLined workflow with no ribosomal RNA depletion requirement or a globin reduction step for blood samples
• Opportunities to bundle WT arrays with WT reagents, offering exceptional cost savings
• Convenient single source for ordering and support

Related Links
GeneChip HT WT PLUS Reagent Kit
HT WT PLUS Consumables for Biomek FXP Target Prep Express
For Research Use Only. Not for use in diagnostic procedures.
Specifications
For Use With (Application)Transcriptome Profiling
Product LineGeneChip
Quantity30 Reactions
Reagent TypeSample preparation reagents
TypeWT PLUS Reagent Kit
Unit SizeEach

Frequently asked questions (FAQs)

Where can I download the control sequences for expression arrays?

Sequences of all our controls can be downloaded from the sequence file that is available on product pages on thermofisher.com.

For example, here is the link to the product page for Clariom D Assay, human: https://www.thermofisher.com/order/catalog/product/902923
Go to the "Software & Data Analysis" section and under "Support Files", click on "Sequence Files: Clariom D Array, human Probe Sequences, tabular format", which you can open in Excel.

Note: If the control sequence file is not available, please contact techsupport@thermofisher.com.

What is the shelf life of the Poly-A RNA Control's first dilution during processing of expression microarrays?

The first dilution of the Poly-A RNA Control can be stored at -20 degrees C for 6 weeks (with up to 4 freeze/thaws).

What is the shelf life for expression microarrays?

Typical shelf life for expression arrays are as follows:
- All ≥ 8 µm commercial expression cartridges are stable up to 24 months.
- All ≥ 8 µm commercial resequencing cartridges are stable up to 18 months.
- All ≥ 8 µm commercial expression plates are stable up to 18 months.

Please note that this excludes products such as Exon or Gene array as they are 5 µm cartridge and plates.

Is there a recommended RIN (RNA integrity number), based on Bioanalyzer, for RNA quality for IVT and WT assays?

For IVT assays, the RIN should be 6 or higher. For WT assays, there are no recommendations based on assay priming.

Find additional tips, troubleshooting help, and resources within our Microarray Analysis Support Center.

Are there stopping points for the gene expression workflow that allow for storage at -20 degrees C?

All senseRNA, cRNA, and cDNA products generated using GeneChip WT PLUS Reagent Kit and GeneChip 3' IVT PLUS Reagent Kit can be stored at -20 degrees C.

Find additional tips, troubleshooting help, and resources within our Microarray Analysis Support Center.