Sulfo-EGS (ethylene glycol bis(sulfosuccinimidyl succinate))
Sulfo-EGS (ethylene glycol bis(sulfosuccinimidyl succinate))
Citas y referencias (7)
Thermo Scientific™

Sulfo-EGS (ethylene glycol bis(sulfosuccinimidyl succinate))

El Sulfo-EGS Thermo Scientific Pierce es un entrecruzador soluble en agua que contiene extremos de éster sulfo-NHS reactivos con aminasMás información
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Número de catálogoCantidad
2156650 mg
Número de catálogo 21566
Precio (MXN)
-
Cantidad:
50 mg
Pedido a granel o personalizado
El Sulfo-EGS Thermo Scientific Pierce es un entrecruzador soluble en agua que contiene extremos de éster sulfo-NHS reactivos con aminas alrededor de un brazo espaciador de 12 átomos, que se puede escindir mediante un tratamiento con hidroxilamina a pH 8,5.

Características del Sulfo-EGS:

• Grupos reactivos: Éster Sulfo-NHS (ambos extremos)
• Reactivo a: grupos amino (aminas primarias)
• Soluble en agua; compare con EGS
• Membrana impermeable, permitiendo el etiquetado de superficie celular
• Los entrecruzados formados son reversibles a pH 8,5 usando hidroxilamina durante un periodo de 3 a 6 horas a 37 °C
• La lactosa deshidrogenasa retuvo el 60 % de su actividad después del entrecruzamiento reversible con EGS

Referencias de producto:
Guía de aplicación entrecruzada: búsqueda de referencias literarias recientes para este producto

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Sulfo-EGS (etilenglicol bis(sulfosucinimidil succinato))
Para uso exclusivo en investigación. No apto para uso en procedimientos diagnósticos.
Especificaciones
Permeabilidad celularNo
DescripciónSulfo-EGS
FormularioPolvo
Método de etiquetadoEtiqueta química
Peso molecular660.45
PegiladoNo
Línea de productosPierce
Cantidad50 mg
Fracción reactivaÉster Suffo-NHS
Condiciones de envíoAmbiente
SolubilidadAgua
Longitud del brazo del separador16,1 Å
Soluble en aguaSí
Reactividad químicaAmina-amina
CleavablePor hidroxilamina
Tipo de enlace cruzadoHomobifuncionales
FormatoEstándar
Tipo de productoEntrecruzador
SeparadorMedio (de 10 a 30 Å)
Unit SizeEach
Contenido y almacenamiento
Tras su recepción, almacenar desecado a -20 °C.

Preguntas frecuentes

Can you provide the shelf-life for Sulfo-EGS (ethylene glycol bis(sulfosuccinimidyl succinate))?

Sulfo-EGS (ethylene glycol bis(sulfosuccinimidyl succinate)) is covered under our general 1-year warranty and is guaranteed to be fully functional for 12 months from the date of shipment, if stored as recommended. Please see section 8.1 of our Terms & Conditions of Sale (https://www.thermofisher.com/content/dam/LifeTech/Documents/PDFs/Terms-and-Conditions-of-Sale.pdf) for more details.

Find additional tips, troubleshooting help, and resources within our Protein Purification and Isolation Support Center.

Citations & References (7)

Citations & References
Abstract
Role of TRIM5a RING domain E3 ubiquitin ligase activity in capsid disassembly, reverse transcription blockade, and restriction of simian immunodeficiency virus.
Authors:Kim J, Tipper C, Sodroski J
Journal:J Virol
PubMed ID:21680520
'The mammalian tripartite motif protein, TRIM5a, recognizes retroviral capsids entering the cytoplasm and blocks virus infection. Depending on the particular TRIM5a protein and retrovirus, complete disruption of the TRIM5a RING domain decreases virus-restricting activity to various degrees. TRIM5a exhibits RING domain-dependent E3 ubiquitin ligase activity, but the specific role of ... More
A novel C-terminal mutation resulting in constitutive activation of the Listeria monocytogenes central virulence regulatory factor PrfA.
Authors:Xayarath B, Smart JI, Mueller KJ, Freitag NE
Journal:Microbiology
PubMed ID:21835879
'The environmental bacterium Listeria monocytogenes survives and replicates in a variety of diverse ecological niches that range from the soil to the cytosol of infected mammalian cells. The ability of L. monocytogenes to replicate within an infected host requires the expression of a number of secreted bacterial gene products whose ... More
Raf kinase inhibitor protein (RKIP) dimer formation controls its target switch from Raf1 to G protein-coupled receptor kinase (GRK) 2.
Authors:Deiss K, Kisker C, Lohse MJ, Lorenz K
Journal:J Biol Chem
PubMed ID:22610096
'Proteins controlling cellular networks have evolved distinct mechanisms to ensure specificity in protein-protein interactions. Raf kinase inhibitor protein (RKIP) is a multifaceted kinase modulator, but it is not well understood how this small protein (21 kDa) can coordinate its diverse signaling functions. Raf1 and G protein-coupled receptor kinase (GRK) 2 ... More
Syk-dependent phosphorylation of CLEC-2: a novel mechanism of hem-immunoreceptor tyrosine-based activation motif signaling.
Authors:Séverin S, Pollitt AY, Navarro-Nuñez L, Nash CA, Mourão-Sá D, Eble JA, Senis YA, Watson SP
Journal:J Biol Chem
PubMed ID:21098033
The C-type lectin-like receptor CLEC-2 signals via phosphorylation of a single cytoplasmic YXXL sequence known as a hem-immunoreceptor tyrosine-based activation motif (hemITAM). In this study, we show that phosphorylation of CLEC-2 by the snake toxin rhodocytin is abolished in the absence of the tyrosine kinase Syk but is not altered ... More
Structural basis of molecular recognition of the Leishmania small hydrophilic endoplasmic reticulum-associated protein (SHERP) at membrane surfaces.
Authors:Moore B, Miles AJ, Guerra-Giraldez C, Simpson P, Iwata M, Wallace BA, Matthews SJ, Smith DF, Brown KA
Journal:J Biol Chem
PubMed ID:21106528
The 57-residue small hydrophilic endoplasmic reticulum-associated protein (SHERP) shows highly specific, stage-regulated expression in the non-replicative vector-transmitted stages of the kinetoplastid parasite, Leishmania major, the causative agent of human cutaneous leishmaniasis. Previous studies have demonstrated that SHERP localizes as a peripheral membrane protein on the cytosolic face of the endoplasmic ... More
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