pTK-Green Renilla Luc Vector for Luciferase Assays
pTK-Green Renilla Luc Vector for Luciferase Assays
Thermo Scientific™

pTK-Green Renilla Luc Vector for Luciferase Assays

The Thermo Scientific pCMV-Green Renilla Luc vector is a derivative of pMCS-Green Renilla Luc. It contains the luciferase gene under深入閱讀
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產品號碼Quantity
1615410 μg
產品號碼 16154
價格 (TWD)
4,250.00
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Ends: 31-Dec-2025
6,060.00
您節省 1,810.00 (30%)
Each
新增至購物車
Quantity:
10 μg
價格 (TWD)
4,250.00
Online offer
Ends: 31-Dec-2025
6,060.00
您節省 1,810.00 (30%)
Each
新增至購物車
The Thermo Scientific pCMV-Green Renilla Luc vector is a derivative of pMCS-Green Renilla Luc. It contains the luciferase gene under the control of the Herpes Simplex Virus (HSV) thymidine kinase (TK) promoter, creating a constitutive expression vector that can be used as a normalization control to account for experimental variation in combination with other reporters.

Features of the pTK-Green Renilla Luc vector:

• Weak (TK) constitutive promoter for co-transfection and normalization
• Intracellular Green Renilla luciferase gene, optimized for high expression in mammalian systems
• Transcription termination site (Ter), Lac operator (Lac O1), and transcriptional pause site (TPS) used to minimize background by reducing transcriptional read-through
• Both puromycin (Pur) and ampicillin (Amp) markers for drug selection in mammalian and bacterial cells, respectively
• High-copy pUC bacterial DNA replication origin

More Product Data
• Luciferase assays in hard-to-transfect Jurkat cells

Related Products
pMCS-Green Renilla Luc Vector for Luciferase Assays
pCMV-Green Renilla Luc Vector for Luciferase Assays
For Research Use Only. Not for use in diagnostic procedures.
規格
Cloning MethodRestriction Enzyme/MCS
Delivery TypeTransfection
DescriptionpTK-Green Renilla Luc Vector
For Use With (Application)Reporter Assays
Product LinePierce
Quantity10 μg
Reporter GeneGreen Renilla Luciferase
Selection Agent (Eukaryotic)Puromycin
PromoterTK
TypeLuc Vector
Unit SizeEach
內容物與存放
Store in original container protected from direct sunlight in a dry, cool and well-ventilated area, between the following temperatures: -70 to -20°C.

常見問答集 (常見問題)

What options do you offer for luciferase vectors?

There are three vectors available for each expressed luciferase; pMCS, pCMV, and pTK. We recommend using pMCS to measure the activity of the reporter, and pCMV, and pTK as positive control vectors. For high level constitutive expression of Luciferase, we recommend using the CMV (Cytomegalovirus) promoter vector and for low level constitutive expression of luciferase, we recommend using the pTK (thymidine kinase) promoter vector.

Find additional tips, troubleshooting help, and resources within our Protein Assays and Analysis Support Center.

What is the stability of the secreted luciferase?

Gaussia, Gaussia-Dura, and Cypridina Luciferase are stable in the culture media for greater than 24 hours. Generally, after transfection of CMV driven constructs, we see ever increasing secreted signal between 24 and 72 hours.

Find additional tips, troubleshooting help, and resources within our Protein Assays and Analysis Support Center.

How quickly is luciferase secreted?

The earliest detectable signal will depend on the strength of the promoter. In general, with a strong promoter (such as CMV), significant signal over background can be seen in as little as 20 minutes. However, with a weaker inducible promoter, significant signal over background may take 1-2 hours after induction.

Find additional tips, troubleshooting help, and resources within our Protein Assays and Analysis Support Center.

Do you still offer quantitative IP (qIP) Luciferase assay kits?

Our qIP Luciferase assay kits have been discontinued but we do carry epitope-tagged (HA or c-Myc) and Tluc (TurboLuc luciferase)-tagged mammalian expression vectors, and qIP protein interaction assay reagents for these assay kits.

Find additional tips, troubleshooting help, and resources within our Protein Assays and Analysis Support Center.