Cytokine 25-Plex Human Panel

The Cytokine Human Magnetic 25-Plex Panel for the Luminex™ platform is specifically designed for quantifying human cytokines and chemokines in serum, plasma, and tissue culture supernatant. By measuring 25 analytes simultaneously, the Luminex™ assay panel helps provide more data from each sample, saving both money and time compared to more traditional systems (such as ELISA) used in research. This assay may be run alone or in combination with other select Luminex™ singleplex kits. The panel uses magnetic beads, facilitating automation, decreasing hands-on time, and increasing throughput and precision. The panel is suitable for use with the Luminex™ 100™/200™, FLEXMAP 3D™, and MAGPIX™ systems. Advantages of the Cytokine Human Magnetic 25-Plex Panel:

• Superior performance—accurate, reproducible, and sensitive quantitation of multiple proteins
• High quality—fully qualified antibodies permit excellent specificity and sensitivity
• Fast and easy protocols—perform your multiplex assay and analyze your data typically in less than one day

Comprehensive human analyte panel
The Human Cytokine Magnetic 25-Plex Panel for the Luminex™ platform provides reagents for the accurate, reproducible, and sensitive quantitation of human proteins including:

Cytokines
GM-CSF
IFN-α
IFN-γ
IL-1β
IL-1RA
IL-2
IL-2R
IL-4
IL-5
IL-6
IL-7
IL-8
IL-10
IL-12 (p40/p70)
IL-13
IL-15
IL-17
TNF-α

Chemokines
Eotaxin
IP-10
MCP-1
MIG
MIP-1α
MIP-1β
RANTES

Luminex™ xMAP™ technology—effective and proven analytical tool
The Human Cytokine Magnetic 25-Plex Panel is based on xMAP™ technology. The use of a suspension bead-based technology enables the multiplexing capabilities of the Luminex™ assays. Magnetic microspheres are internally dyed with red and infrared fluorophores of differing intensities. Each bead is given a unique number, or bead region, allowing differentiation of one bead from another. Beads covalently bound to different antibodies can be mixed in the same assay, using a 96-well microplate format. Upon completion of the sandwich immunoassay, magnetic beads must be measured using a Luminex™ detection system (MAGPIX™, 100™/200™, or FLEXMAP 3D™). The instrument uses xPONENT™ software to distinguish bead color (analyte) and R-PE fluorescence intensity (assay signal strength) to quantify target(s).

Magnetic beads let you do more with your time
Magnetic bead-based assays use Luminex™ MagPlex magnetic microspheres. All other assay components are the same as the equivalent Luminex™ polystyrene bead-based assays providing identical quality and consistency performance. MagPlex technology takes advantage of magnetic properties to simplify assay wash steps and maximize uniformity of results. Magnetic bead-based assays also enable automation, decrease hands-on time, and increase throughput and precision. Easy-to-use protocols allow you to get your results in as little as 3.5 hours. In addition, these assays are compatible with both vacuum and magnetic wash stations and work well with the MAGPIX™ system as well as other Luminex™ xMAP™ platforms.

More validation for confidence in your results
The Human Cytokine Magnetic 25-Plex Panel has been validated using the same rigorous criteria that distinguishes Luminex™ assays from other commercially available assays. With more than 20 years of experience, our fully qualified antibodies help ensure the specificity and sensitivity of our assays. When benchmarked against the competition, our multiplex products consistently deliver excellent results. Every singleplex kit and premixed multiplex kit comes with a product insert that outlines the specifications for each marker in the assay (see documents section).

Learn more about all Invitrogen™ assays for the Luminex™ platform.