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Invitrogen
IL-17A Monoclonal Antibody (eBio64CAP17), PE, eBioscience™
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FIGURE: 1 / 14
IL-17A Antibody (12-7178-42) in Flow
Normal human peripheral blood cells were stimulated overnight with PMA and Ionomycin in the presence of monensin. Cells were stained with Anti-Human CD3 FITC (Product # 11-0037-42), then fixed, permeablized, and stained with Anti-Human IL-17A PE (Left figure). The ligand blocking control demonstrates that pre-blocking Anti-Human IL-17A PE with 1.0 µg of Human IL-17A Recombinant Protein (Product # 14-8179-80) prior to staining abrogates specific IL-17A staining (Right figure). Cells in the lymphocyte gate were used for analysis.
Product Details
12-7178-42
Applications
Tested Dilution
Publications
Product Specifications
Species Reactivity
Human
Published species
Cynomolgus monkey,
Human,
Mouse,
Non-human primate,
Rhesus monkey
Host/Isotype
Mouse
/ IgG1, kappa
Recommended Isotype Control
Class
Monoclonal
Type
Antibody
Clone
eBio64CAP17
Conjugate
Excitation/Emission Max
565/576 nm
View spectra
Form
Liquid
Concentration
5 µL/Test
Purification
Affinity chromatography
Storage buffer
PBS, pH 7.2, with BSA
Contains
0.09% sodium azide
Storage conditions
4°C, store in dark, DO NOT FREEZE!
Shipping conditions
Ambient (domestic); Wet ice (international)
RRID
Product Specific Information
Description: The eBio64CAP17 antibody reacts with human IL-17A; the antibody has been reported to cross react with Rhesus monkey IL-17A, as verified by intracellular staining experiments. The eBio64CAP17 antibody is a neutralizing antibody. This antibody has been shown to have no reactivity to human IL-17F. Reactivity to other members of the IL-17 family has not been evaluated. Interleukin-17A (IL-17A) is a CD4+ T cell-derived cytokine that promotes inflammatory responses in cell lines and is elevated in rheumatoid arthritis, asthma, multiple sclerosis, psoriasis, and transplant rejection. The cDNA encoding human IL-17A was isolated from a library of CD4+ T cells; the encoded protein exhibits 72 percent amino acid identity with HVS13 , an open reading frame from a T lymphotropic Herpesvirus saimiri, and 63 percent with mouse CTLA-8 (cytotoxic T-lymphocyte associated antigen-8). Human IL-17A exists as glycosylated 20-30 kD homodimers. High levels of IL-17A homodimer are produced by activated peripheral blood CD4+ T-cells. IL-17A enhances expression of the intracellular adhesion molecule-1 (ICAM-1) in human fibroblasts. Human IL-17A also stimulates epithelial, endothelial, or fibroblastic cells to secrete IL-6, IL-8, G-CSF, and PGE2. In the presence of human IL-17A, fibroblasts can sustain the proliferation of CD34+ hematopoietic progenitors and induce maturation into neutrophils. Mouse, rat, and human IL-17A can induce IL-6 secretion in mouse stromal cells, indicating that all homologs can recognize the mouse IL-17A receptor.
IL-23-dependent, IL-17A-producing CD4+ T cells (Th-17 cells) have been identified as a unique subset of Th cells that develops along a pathway that is distinct from the Th1- and Th2- cell differentiation pathways. The hallmark effector molecules of Th1 and Th2 cells, e.g., IFN-g and IL-4, have each been found to negatively regulate the generation of these Th-17 cells.
Additionally, activated human CD4+ T cells have been found to produce the IL-17A/F heterodimer, as well as the corresponding homodimers. In comparing the relative potency of IL-17A, IL-17F, and IL-17A/F, all three were found to induce GRO-a secretion; IL-17A was most potent, followed by IL-17A/F heterodimer, then IL-17F (100fold lower than IL-17A). eBio64CAP17 can be used to detect IL-17 heterodimers by immunoprecipitation followed by immunoblot withH17F10A7 anti-IL17F monoclonal antibody.
The eBio64CAP17 has been shown to react to rhesus and marmoset primates.
Applications Reported: The eBio64CAP17 antibody has been reported for use as the capture antibody in human IL-17A ELISA and ELISPOT assays, for neutralization of IL-17A bioactivity, and for intracellular staining and flow cytometric analysis of IL-17A-producing cells.
Applications Tested: This eBio64CAP17 antibody has been pre-titrated and tested by intracellular staining for flow cytometric analysis of stimulated normal human peripheral blood cells. This can be used at 5 µL (0.25 µg) per test. A test is defined as the amount (µg) of antibody that will stain a cell sample in a final volume of 100 µL. Cell number should be determined empirically but can range from 10^5 to 10^8 cells/test.
Excitation: 488-561 nm; Emission: 578 nm; Laser: Blue Laser, Green Laser, Yellow-Green Laser.
Filtration: 0.2 µm post-manufacturing filtered.
Target Information
Interleukin-17A (IL-17A, CTLA-8) is a CD4+ T cell-derived cytokine that promotes inflammatory responses in cell lines and is elevated in rheumatoid arthritis, asthma, multiple sclerosis, psoriasis, and transplant rejection. IL-17A is a 32 kDa long, disulfide-linked homodimer consisting of 136 amino acids that is a member of a six-species family of proteins (IL-17A-17F) and signals through the IL-17 receptor (IL-17R/CDw217). High levels of IL-17A homodimer are produced by activated peripheral blood CD4+ T-cells, and IL-17A also enhances expression of the intracellular adhesion molecule-1 (ICAM-1) in human fibroblasts. In particular, human IL-17A also stimulates epithelial, endothelial, or fibroblastic cells to secrete IL-6, IL-8, G-CSF, and PGE2. In the presence of human IL-17A, fibroblasts can sustain the proliferation of CD34+ hematopoietic progenitors and induce maturation into neutrophils. Mouse, rat, and human IL-17A can induce IL-6 secretion in mouse stromal cells, indicating that all homologs can recognize the mouse receptor. IL-17A regulates the activities of NF-kappa B and mitogen-activated protein kinases, stimulates the expression of IL-6 and cyclooxygenase-2 (PTGS2/COX-2), and enhances the production of nitric oxide (NO).
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
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Bioinformatics
Protein Aliases: CTLA-8; Cytotoxic T-lymphocyte-associated antigen 8; cytotoxic T-lymphocyte-associated protein 8; il 17; IL-17; ILN; inflammatory cytokine; Interleukin; interleukin 17; interleukin 17 (cytotoxic T-lymphocyte-associated serine esterase 8); interleukin-17; Interleukin-17A; Interleukin17A; R-IL-17-A
Gene Aliases: CTLA-8; CTLA8; IL-17; IL-17A; IL17; IL17A; ILA17
UniProt ID: (Human) Q16552
Entrez Gene ID: (Human) 3605
positive regulation of antimicrobial peptide production
adaptive immune response
immune system process
apoptotic process
inflammatory response
immune response
Notch signaling pathway
cell-cell signaling
cell death
response to wounding
gene expression
positive regulation of keratinocyte proliferation
keratinocyte differentiation
positive regulation of interleukin-1 beta production
positive regulation of interleukin-12 production
positive regulation of interleukin-16 production
positive regulation of interleukin-23 production
positive regulation of interleukin-6 production
positive regulation of tumor necrosis factor production
interleukin-17A-mediated signaling pathway
keratinocyte proliferation
innate immune response
positive regulation of osteoclast differentiation
positive regulation of transcription from RNA polymerase II promoter
defense response to Gram-negative bacterium
defense response to Gram-positive bacterium
defense response to fungus
positive regulation of protein kinase B signaling
intestinal epithelial structure maintenance
cellular response to interleukin-1
fibroblast activation
regulation of primary metabolic process
interleukin-17-mediated signaling pathway
granulocyte migration
negative regulation of inflammatory response to wounding
positive regulation of cytokine production involved in inflammatory response
positive regulation of bicellular tight junction assembly
positive regulation of tumor necrosis factor superfamily cytokine production
negative regulation of interleukin-17-mediated signaling pathway
positive regulation of chemokine (C-X-C motif) ligand 1 production
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