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Invitrogen
CD106 (VCAM-1) Monoclonal Antibody (STA), eBioscience™
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FIGURE: 1 / 13
CD106 (VCAM-1) Antibody (14-1069-82) in ICC/IF
Nature communications 2017 -
Figure 1 Enhanced expression of endothelial SENP1 and GATA2 correlates with graft arteriosclerosis (GA) progression. Similarly sized human coronary arteries with GA from chronically rejecting heart allografts or without disease from non-transplanted hearts were collected and evaluated by histological analysis. ( a , b ) Dramatically increased expression of endothelial SENP1 was detected in the diseased vess... View More
Product Details
14-1069-82
Applications
Tested Dilution
Publications
Product Specifications
Species Reactivity
Human
Published species
Human,
Mouse
Host/Isotype
Mouse
/ IgG1, kappa
Class
Monoclonal
Type
Antibody
Clone
STA
Conjugate
Form
Liquid
Concentration
0.5 mg/mL
Amount
100 µg
Purification
Affinity chromatography
Storage buffer
PBS, pH 7.2
Contains
0.09% sodium azide
Storage conditions
4°C
Shipping conditions
Ambient (domestic); Wet ice (international)
RRID
Product Specific Information
Description: The STA monoclonal antibody reacts with human CD106 (Vascular Cell Adhesion Molecule-1, VCAM-1), a 110 kDa transmembrane glycoprotein expressed by myeloid lineage and bone marrow stromal cells. Endothelial cells constitutively express low levels of CD106 and upregulate it upon cytokine stimulation. CD106 binds to Integrin alpha4beta1 (VLA-4, CD49d/CD29) and Integrin alpha4beta7 (LPAM-1). Cytokine-mediated upregulation of CD106 on endothelial cells suggests a role for this antigen in the inflammatory response.
Applications Reported: STA has been reported for use in flow cytometric analysis, and immunohistochemical staining of frozen tissue sections.
Applications Tested: The STA antibody has been tested by flow cytometric analysis of TNF alpha-activated HUVEC cells. This can be used at less than or equal to 1 µg per test. A test is defined as the amount (µg) of antibody that will stain a cell sample in a final volume of 100 µL. Cell number should be determined empirically but can range from 10^5 to 10^8 cells/test. It is recommended that the antibody be carefully titrated for optimal performance in the assay of interest.
Purity: Greater than 90%, as determined by SDS-PAGE.
Aggregation: Less than 10%, as determined by HPLC.
Filtration: 0.2 µm post-manufacturing filtered.
Target Information
VCAM-1 is a cell surface sialoglycoprotein expressed by cytokine activated endothelium. The protein has a number of functions including the regulation of leukocyte migration, leukocyte-endothelial cell adhesion and signal transduction and may play a role in a number of inflammatory diseases.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
Bioinformatics
Protein Aliases: CD106; CD106 antigen; DKFZp779G2333; INCAM-100; INCAM100; MGC99561; sCD106; soluble CD106; soluble VCAM 1; sVCAM 1; unnamed protein product; V-CAM 1; vascular cell adhesion molecule 1 precursor; Vascular cell adhesion protein 1
Gene Aliases: CD106; INCAM-100; VCAM1
UniProt ID: (Human) P19320
Entrez Gene ID: (Human) 7412
response to hypoxia
chronic inflammatory response
inflammatory response
cell adhesion
heterophilic cell-cell adhesion via plasma membrane cell adhesion molecules
leukocyte cell-cell adhesion
cell-matrix adhesion
response to nutrient
amine metabolic process
response to zinc ion
response to ionizing radiation
membrane to membrane docking
B cell differentiation
response to lipopolysaccharide
cell-cell adhesion mediated by integrin
heterotypic cell-cell adhesion
response to nicotine
cellular response to vascular endothelial growth factor stimulus
positive regulation of T cell proliferation
response to ethanol
leukocyte tethering or rolling
cell chemotaxis
innervation
cardiac neuron differentiation
cellular response to tumor necrosis factor
cell-cell adhesion
cellular response to beta-amyloid
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Performance Guarantee
If an Invitrogen™ antibody doesn't perform as described on our website or datasheet,we'll replace the product at no cost to you, or provide you with a credit for a future purchase.*
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