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Invitrogen
CD106 (VCAM-1) Monoclonal Antibody (STA), Biotin, eBioscience™
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FIGURE: 1 / 13
CD106 (VCAM-1) Antibody (13-1069-82) in Flow
Staining of TNF alpha-stimulated Human Umbilical Vein Endothelial Cells (HUVEC) cells with 0.5 µg of Mouse IgG1 kappa Isotype Control Biotin (Product # 13-4714-85) (open histogram) or 0.5 µg of Anti-Human CD106 (VCAM-1) Biotin (filled histogram) followed by Streptavidin PE (Product # 12-4317-87). Total viable cells were used for analysis.
Product Details
13-1069-82
Applications
Tested Dilution
Publications
Product Specifications
Species Reactivity
Human
Published species
Human
Host/Isotype
Mouse
/ IgG1, kappa
Recommended Isotype Control
Class
Monoclonal
Type
Antibody
Clone
STA
Conjugate
Form
Liquid
Concentration
0.5 mg/mL
Purification
Affinity chromatography
Storage buffer
PBS, pH 7.2
Contains
0.09% sodium azide
Storage conditions
4°C, store in dark, DO NOT FREEZE!
Shipping conditions
Ambient (domestic); Wet ice (international)
RRID
Product Specific Information
Description: The STA monoclonal antibody reacts with human CD106 (Vascular Cell Adhesion Molecule-1, VCAM-1), a 110 kDa transmembrane glycoprotein expressed by myeloid lineage and bone marrow stromal cells. Endothelial cells constitutively express low levels of CD106 and upregulate it upon cytokine stimulation. CD106 binds to Integrin alpha4beta1 (VLA-4, CD49d/CD29) and Integrin alpha4beta7 (LPAM-1). Cytokine-mediated upregulation of CD106 on endothelial cells suggests a role for this antigen in the inflammatory response.
Applications Reported: STA has been reported for use in flow cytometric analysis.
Applications Tested: The STA antibody has been tested by flow cytometric analysis of TNF alpha-activated HUVEC cells. This can be used at less than or equal to 0.5 µg per test. A test is defined as the amount (µg) of antibody that will stain a cell sample in a final volume of 100 µL. Cell number should be determined empirically but can range from 10^5 to 10^8 cells/test. It is recommended that the antibody be carefully titrated for optimal performance in the assay of interest.
Filtration: 0.2 µm post-manufacturing filtered.
Target Information
VCAM-1 is a cell surface sialoglycoprotein expressed by cytokine activated endothelium. The protein has a number of functions including the regulation of leukocyte migration, leukocyte-endothelial cell adhesion and signal transduction and may play a role in a number of inflammatory diseases.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
Bioinformatics
Protein Aliases: CD106; CD106 antigen; DKFZp779G2333; INCAM-100; INCAM100; MGC99561; sCD106; soluble CD106; soluble VCAM 1; sVCAM 1; V-CAM 1; Vascular cell adhesion protein 1
Gene Aliases: CD106; INCAM-100; VCAM1
UniProt ID: (Human) P19320
Entrez Gene ID: (Human) 7412
response to hypoxia
acute inflammatory response
chronic inflammatory response
cell adhesion
heterophilic cell-cell adhesion via plasma membrane cell adhesion molecules
leukocyte cell-cell adhesion
cell-matrix adhesion
aging
response to nutrient
amine metabolic process
response to zinc ion
response to ionizing radiation
viral process
membrane to membrane docking
B cell differentiation
extracellular matrix organization
response to lipopolysaccharide
response to nicotine
calcium-mediated signaling using intracellular calcium source
cellular response to vascular endothelial growth factor stimulus
positive regulation of T cell proliferation
response to ethanol
regulation of immune response
leukocyte tethering or rolling
oxidation-reduction process
cell chemotaxis
interferon-gamma-mediated signaling pathway
cellular response to tumor necrosis factor
Performance Guarantee
If an Invitrogen™ antibody doesn't perform as described on our website or datasheet,we'll replace the product at no cost to you, or provide you with a credit for a future purchase.*
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